Detailed information NTG0544935

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J0W33_RS01385
• Genome accession: NZ_CP071319
• Coordinates: 293546..294739 (-)
• Length: 397 a.a.
• NCBI ID: WP_045794791.1
• Uniprot ID: A0A6C0Y670
• Organism: Acinetobacter indicus strain GXNN62X4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 288546..299739

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J0W33_RS01365 (J0W33_01365)	-	290026..290475 (+)	450	WP_016658087.1	MaoC family dehydratase	-
J0W33_RS01370 (J0W33_01370)	-	290579..291607 (+)	1029	WP_005178175.1	helix-turn-helix transcriptional regulator	-
J0W33_RS01375 (J0W33_01375)	gcvH	291676..292050 (+)	375	WP_005178176.1	glycine cleavage system protein GcvH	-
J0W33_RS01380 (J0W33_01380)	-	292306..293487 (+)	1182	WP_206896882.1	MFS transporter	-
J0W33_RS01385 (J0W33_01385)	pilU	293546..294739 (-)	1194	WP_045794791.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
J0W33_RS01390 (J0W33_01390)	-	294993..296048 (-)	1056	WP_104471376.1	3-deoxy-7-phosphoheptulonate synthase	-
J0W33_RS01395 (J0W33_01395)	hppD	296924..297982 (+)	1059	WP_016658083.1	4-hydroxyphenylpyruvate dioxygenase	-
J0W33_RS01400 (J0W33_01400)	-	298134..299516 (+)	1383	WP_016658082.1	amino acid permease	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44672.05 Da
• Isoelectric Point: 6.2356

>NTDB_id=544935 J0W33_RS01385 WP_045794791.1 293546..294739(-) (pilU) [Acinetobacter indicus strain GXNN62X4]
MYSAELLEDARKFMFHMLSKVVEFGGSDLFITADFPPSIKHQGMMKALGQQALTAEKTKLFAYSLMNEKQRSEFETDLEC
NFAISVPEVSRFRVNVFQQQLHVGMVIRTISAEIPNFSQLKLPESLKHIIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDSHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTEDGKGRRAAVEIMLNTPLISELILKGQFHELKSIMGKS
RELGMQTFDQALFDLYNEGAISYEEAIRNADSMNELRLQIKLKSNRPSEASAAMSFSMLEEPKPEDEDTESAPQQSA

Nucleotide

• Length: 1194 bp

>NTDB_id=544935 J0W33_RS01385 WP_045794791.1 293546..294739(-) (pilU) [Acinetobacter indicus strain GXNN62X4]
ATGTACAGTGCAGAATTACTCGAAGATGCGCGTAAGTTTATGTTTCATATGCTCAGCAAAGTGGTTGAGTTTGGGGGATC
AGATTTATTTATTACCGCGGATTTTCCACCGAGCATTAAACATCAGGGCATGATGAAAGCGCTGGGACAACAGGCGCTGA
CCGCAGAAAAAACCAAGCTGTTTGCCTACAGCCTCATGAATGAAAAACAGCGTAGCGAGTTTGAAACTGATCTGGAATGC
AACTTTGCCATTAGTGTGCCGGAGGTATCGCGTTTTCGGGTCAATGTGTTTCAACAGCAGCTGCATGTCGGGATGGTGAT
TCGAACCATTAGCGCGGAAATTCCAAACTTTAGCCAGCTGAAACTGCCGGAATCACTGAAGCATATTATTATGGAAAAGC
GCGGTCTGGTGCTGGTGGTGGGGGGAACCGGTTCAGGTAAATCGACCTCTTTGGCGGCGATGATTGATCATCGTAATGAA
AATTCAGCCGGGCATATTATTACCGTGGAAGACCCGGTGGAATATGTGCACAAGCATAAAAAGTCGATGATTACCCATCG
TGAAGTCGGGGTCGACAGCCATTCCTGGCACAATGCGCTGAAAAATACCTTACGTCAGGCACCGGATGTGATTTTAATTG
GCGAGATCCGCGACACCGAAACCATGGAACATGCGATTGCCTTTGCTGAAACCGGGCATTTATGTTTGGGCACCTTGCAT
GCCAATAACGCCAACCAGACGCTGGACCGGATTATCAACTTTTTCCCGGAAGAGCGCCGTAACCAGTTGCTGATGGATCT
GTCGTCCAATATGAAGGCCATTATTTCCCAGCGTCTGGTGCGTACCGAAGATGGTAAAGGCCGCCGTGCGGCAGTCGAGA
TTATGCTGAATACGCCGCTGATTTCTGAACTGATTTTAAAAGGCCAGTTCCATGAGTTAAAGTCGATTATGGGCAAATCG
CGTGAACTGGGCATGCAGACGTTTGATCAGGCTCTATTTGATTTGTATAACGAAGGCGCGATTTCCTATGAAGAAGCAAT
TCGTAATGCCGATTCGATGAATGAATTGCGCTTACAGATTAAATTGAAAAGTAACCGGCCAAGCGAAGCTTCGGCAGCTA
TGAGCTTTAGCATGCTGGAAGAGCCAAAACCGGAAGATGAAGATACGGAATCTGCTCCTCAACAGAGTGCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (117-282)

3D structure

• AlphaFold DB: A0A6C0Y670

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.568	93.199	0.537
pilU	Acinetobacter baylyi ADP1	54.42	91.184	0.496
pilU	Vibrio cholerae strain A1552	52.663	85.139	0.448
pilT	Acinetobacter baumannii strain A118	43.939	83.123	0.365
pilT	Acinetobacter nosocomialis M2	43.939	83.123	0.365
pilT	Acinetobacter baumannii D1279779	43.939	83.123	0.365
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.814	84.131	0.36