Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J0W32_RS09635 Genome accession   NZ_CP071317
Coordinates   2042292..2043998 (-) Length   568 a.a.
NCBI ID   WP_168370330.1    Uniprot ID   -
Organism   Acinetobacter indicus strain GXNN15X4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2037292..2048998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0W32_RS09605 (J0W32_09605) - 2037440..2037763 (+) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -
  J0W32_RS09610 (J0W32_09610) rlmB 2037869..2038621 (+) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  J0W32_RS09615 (J0W32_09615) - 2038639..2039559 (+) 921 WP_016659657.1 DMT family transporter -
  J0W32_RS09620 (J0W32_09620) coaE 2039556..2040164 (-) 609 WP_005181773.1 dephospho-CoA kinase -
  J0W32_RS09625 (J0W32_09625) pilD 2040175..2041035 (-) 861 WP_005181774.1 prepilin peptidase Machinery gene
  J0W32_RS09630 (J0W32_09630) pilC 2041035..2042261 (-) 1227 WP_016659656.1 type II secretion system F family protein Machinery gene
  J0W32_RS09635 (J0W32_09635) pilB 2042292..2043998 (-) 1707 WP_168370330.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J0W32_RS09640 (J0W32_09640) tpiA 2044287..2045078 (+) 792 WP_005181780.1 triose-phosphate isomerase -
  J0W32_RS09645 (J0W32_09645) secG 2045092..2045421 (+) 330 WP_005181783.1 preprotein translocase subunit SecG -
  J0W32_RS09670 (J0W32_09670) rimP 2046291..2046815 (+) 525 WP_005181784.1 ribosome maturation factor RimP -
  J0W32_RS09675 (J0W32_09675) nusA 2046848..2048332 (+) 1485 WP_005181786.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63772.19 Da        Isoelectric Point: 4.8722

>NTDB_id=544923 J0W32_RS09635 WP_168370330.1 2042292..2043998(-) (pilB) [Acinetobacter indicus strain GXNN15X4]
MSVQQAMPRFTGFIRRLVEEGLVTAENMQQAIVAAKKAETDIVPYLIQQLQISPLTIAEKISYEFGEPIFDLAVYDSRLI
VREGLDEKLFTKYRILPIFRRHNQLYIATSNPTNIEAIDAIRFNSKLNIEVVIVEHDKLERLIEQNFTEDSTFDFDEDFD
LEVGDNAPETQEDNDEPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLATRLASRL
KVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQKCKAPVEIPRQSLLELGFTE
EDLNQPEFQLYQPVGCPECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASAKSGFNNLRRSGLIKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=544923 J0W32_RS09635 WP_168370330.1 2042292..2043998(-) (pilB) [Acinetobacter indicus strain GXNN15X4]
ATGTCAGTACAACAAGCAATGCCTAGATTTACAGGTTTTATTCGGCGACTGGTCGAAGAAGGGCTTGTGACGGCTGAAAA
TATGCAGCAAGCCATTGTCGCGGCGAAAAAAGCCGAGACCGATATTGTGCCGTATTTAATTCAGCAGTTGCAGATTTCAC
CGCTGACCATTGCTGAAAAAATTTCCTATGAATTTGGTGAACCGATTTTTGACTTGGCGGTGTACGATTCGCGTCTGATT
GTACGTGAAGGTCTGGATGAAAAACTGTTCACCAAATACCGAATTCTGCCCATTTTCCGACGTCACAATCAGCTTTATAT
CGCTACCAGTAACCCAACCAATATCGAAGCCATTGATGCGATCCGCTTTAACAGCAAGCTGAATATTGAAGTGGTGATTG
TTGAGCATGACAAGCTGGAACGGCTGATTGAACAGAACTTTACCGAAGACAGCACCTTTGACTTTGATGAAGACTTTGAT
CTAGAAGTTGGCGACAACGCTCCCGAAACACAAGAAGATAACGATGAGCCACAAGGCGATGAAGCCCCAATCGTCAAATA
TATTAACAAGTTACTGATCGATGCAATCCGTATGGGCGCCTCGGATTTACACTTCGAGCCTTATGAAAAAAGCTACCGGG
TCCGTTACCGGATTGACGGCGTACTGCGCCAGATTGCCAATCCTCCGTTACAGCTGGCTACACGGCTCGCCTCACGTTTA
AAAGTTATGTCACAAATGGACATCTCGGAAAAACGTATTCCGCAGGATGGCCGGATCAAGCTCAAGCTGTCAAAAAACAA
AGCCATCGACTTCCGTGTCAACTCACTGCCCACCCTGTTCGGGGAAAAAATTGTACTGCGTATTCTCGATCCATCTAGTG
CCATGCTGGGGATTGATGCACTTGGTTATGAACCGGAGCAAAAAGAGCTGTTTATGGAGGCGCTGGACAAACCACAAGGC
ATGTTGCTGATCACCGGCCCGACCGGTTCCGGTAAAACCGTATCTTTATATACCGGCTTAAACATTCTCAACCGCGAAGA
CACCAATATTTCTACCGCCGAAGATCCGGTGGAAATTAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGAAAGTGG
GTCTGACCTTTGCCGCAGCACTAAAATCATTCCTGCGCCAGGATCCGGATATTGTCATGGTCGGTGAGATCCGTGATCTG
GAAACTGCAGAAATTGCCATTAAAGCCGCACAAACCGGTCACATGGTCATGTCGACGCTGCACACCAACAGTGCACCCGA
AACCCTGACCCGTTTACGCAACATGGGCGTCCCCTCATTTAACATCGCCACCTCGGTGAACCTGGTGATTGCCCAGCGTC
TGGCACGTCGTCTGTGTCAGAAATGCAAGGCCCCGGTGGAGATTCCACGCCAGAGCCTCTTGGAACTCGGTTTTACCGAA
GAAGATTTAAACCAGCCAGAGTTCCAGCTGTATCAGCCGGTTGGCTGCCCGGAATGTCGTGAAGGTTATAAAGGCCGTGT
CGGGGTCTATGAAGTAATGAAAGTCACCCCGGAAATCTCACGCATTATTATGGAAGATGGTAATGCCTTGCAAATTGCAG
CAGCCTCGGCAAAATCTGGGTTTAACAATTTACGCCGTTCAGGATTAATCAAGGTCATGCAGGGGGTCACTTCTTTACAG
GAAGTGAATCGTGTGACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.702

100

0.81
  pilB Acinetobacter baylyi ADP1

78.246

100

0.785
  pilF Neisseria gonorrhoeae MS11

51.964

98.592

0.512
  pilB Legionella pneumophila strain ERS1305867

50.53

99.648

0.504
  pilB Vibrio cholerae strain A1552

49.912

100

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

49.553

98.415

0.488
  pilB Vibrio campbellii strain DS40M4

50.864

91.725

0.467
  pilF Thermus thermophilus HB27

37.258

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

33.821

100

0.366