Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J0W32_RS09625 Genome accession   NZ_CP071317
Coordinates   2040175..2041035 (-) Length   286 a.a.
NCBI ID   WP_005181774.1    Uniprot ID   -
Organism   Acinetobacter indicus strain GXNN15X4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2035175..2046035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0W32_RS09605 (J0W32_09605) - 2037440..2037763 (+) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -
  J0W32_RS09610 (J0W32_09610) rlmB 2037869..2038621 (+) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  J0W32_RS09615 (J0W32_09615) - 2038639..2039559 (+) 921 WP_016659657.1 DMT family transporter -
  J0W32_RS09620 (J0W32_09620) coaE 2039556..2040164 (-) 609 WP_005181773.1 dephospho-CoA kinase -
  J0W32_RS09625 (J0W32_09625) pilD 2040175..2041035 (-) 861 WP_005181774.1 prepilin peptidase Machinery gene
  J0W32_RS09630 (J0W32_09630) pilC 2041035..2042261 (-) 1227 WP_016659656.1 type II secretion system F family protein Machinery gene
  J0W32_RS09635 (J0W32_09635) pilB 2042292..2043998 (-) 1707 WP_168370330.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J0W32_RS09640 (J0W32_09640) tpiA 2044287..2045078 (+) 792 WP_005181780.1 triose-phosphate isomerase -
  J0W32_RS09645 (J0W32_09645) secG 2045092..2045421 (+) 330 WP_005181783.1 preprotein translocase subunit SecG -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32048.33 Da        Isoelectric Point: 7.8299

>NTDB_id=544921 J0W32_RS09625 WP_005181774.1 2040175..2041035(-) (pilD) [Acinetobacter indicus strain GXNN15X4]
MQEFIHYLIQHPTALYFVVGIFSLCIGSFLNVVIYRTPKMMEQEWRNDCQMLLHPEQAIIDESKISLSQPASTCPKCHSP
IRWYQNIPVISWLLLRGKCGQCQNPISIRYPFIELLTAVCALTVVAVFGPTLQMLFGLVLTYVLIALTFIDFDTQLLPDR
YTLPLAALGLGVNSYALYTSASSAIWGYIIGFLCLWVVYYLFKLVTGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
VGAIIGIILLKIRKENQPFAFGPYIAIAGWIAFLWGDAIMKLYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=544921 J0W32_RS09625 WP_005181774.1 2040175..2041035(-) (pilD) [Acinetobacter indicus strain GXNN15X4]
ATGCAAGAATTCATTCATTATCTGATTCAACACCCAACTGCGCTGTATTTCGTAGTTGGGATTTTTAGTTTATGTATTGG
CAGTTTTCTGAATGTGGTGATTTACCGCACGCCAAAAATGATGGAACAGGAATGGCGCAATGACTGTCAAATGCTGCTGC
ATCCTGAACAGGCCATCATTGATGAAAGCAAAATCAGCCTCAGTCAGCCAGCCTCGACCTGTCCGAAATGCCACAGCCCG
ATTCGCTGGTATCAGAATATTCCAGTAATCAGCTGGCTGCTTCTACGTGGCAAATGTGGCCAATGCCAAAACCCGATCAG
TATTCGTTATCCTTTTATTGAACTGCTGACCGCAGTGTGTGCCTTAACCGTAGTCGCGGTTTTTGGCCCGACCCTGCAAA
TGCTGTTTGGCCTGGTGTTGACCTATGTGTTAATTGCCCTGACCTTTATTGATTTTGACACCCAGCTGCTGCCTGACCGC
TATACCCTCCCCCTGGCTGCACTCGGCTTGGGGGTGAACAGCTATGCCCTGTACACCTCCGCTAGCTCTGCCATTTGGGG
ATATATCATTGGCTTTTTATGTCTGTGGGTGGTGTATTACCTGTTTAAGCTGGTGACTGGCAAAGAAGGTATGGGCTATG
GCGATTTTAAATTGCTGGCCGCCTTAGGTGCCTGGATGGGCCCGCTGATGCTGCCGCTGATTGTGTTGCTATCTTCTCTG
GTTGGTGCAATCATTGGCATTATTTTGCTGAAAATCCGCAAAGAGAACCAGCCTTTTGCCTTTGGCCCTTATATTGCCAT
TGCCGGCTGGATCGCCTTTTTGTGGGGTGATGCAATCATGAAGCTTTATTTAGGTCAATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

80

99.65

0.797
  pilD Acinetobacter baumannii D1279779

79.649

99.65

0.794
  pilD Vibrio cholerae strain A1552

55.431

93.357

0.517
  pilD Vibrio campbellii strain DS40M4

53.184

93.357

0.497
  pilD Neisseria gonorrhoeae MS11

47.71

91.608

0.437