Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   UC509_RS06225 Genome accession   NC_019435
Coordinates   1231233..1232081 (+) Length   282 a.a.
NCBI ID   WP_015082464.1    Uniprot ID   A0AAJ6N2R3
Organism   Lactococcus cremoris subsp. cremoris UC509.9     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1226233..1237081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UC509_RS06200 (uc509_1241) ilvN 1226966..1227442 (+) 477 WP_015082461.1 acetolactate synthase small subunit -
  UC509_RS06205 (uc509_1242) ilvC 1227488..1228510 (+) 1023 WP_015082462.1 ketol-acid reductoisomerase -
  UC509_RS06210 (uc509_1243) ilvA 1228569..1229819 (+) 1251 WP_014572605.1 threonine ammonia-lyase IlvA -
  UC509_RS06215 (uc509_1244) budA 1229954..1230664 (+) 711 WP_011676302.1 acetolactate decarboxylase -
  UC509_RS06220 (uc509_1245) - 1230704..1231084 (+) 381 WP_015082463.1 RidA family protein -
  UC509_RS06225 (uc509_1246) dprA 1231233..1232081 (+) 849 WP_015082464.1 DNA-processing protein DprA Machinery gene
  UC509_RS06230 (uc509_1247) topA 1232347..1234473 (+) 2127 WP_015082465.1 type I DNA topoisomerase -
  UC509_RS06235 (uc509_1248) trmFO 1234545..1235891 (+) 1347 WP_014572600.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  UC509_RS06240 (uc509_1249) xerS 1235941..1237011 (-) 1071 WP_015082466.1 tyrosine recombinase XerS -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31854.03 Da        Isoelectric Point: 9.8386

>NTDB_id=54460 UC509_RS06225 WP_015082464.1 1231233..1232081(+) (dprA) [Lactococcus cremoris subsp. cremoris UC509.9]
MITNFDLFRWKKAGMTNLGVNKLLKFFLKYDIKISLRQMGQVAQVKSIPNFIEQYKNQDVKKLRIDYKKFSSFSILDDLY
PERLREIYNPPVLIFYQGNIDLLKNPKLAFVGSRLAGQSGIKAVQKIITELNQSFTIVSGLAKGIDTASHLSAIKTKTPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLVLSEYSLGEKPLKYHFPERNRIIAGLSRGVVVVEAKLRSGSLITCERALEEG
RDIFAIPGNIADGTSDGCNHLIQQGAKLVYQAQDILEEYLYN

Nucleotide


Download         Length: 849 bp        

>NTDB_id=54460 UC509_RS06225 WP_015082464.1 1231233..1232081(+) (dprA) [Lactococcus cremoris subsp. cremoris UC509.9]
ATGATTACAAATTTTGATTTATTTCGTTGGAAAAAAGCAGGAATGACCAATCTTGGTGTAAATAAGCTCTTAAAATTTTT
CCTCAAATATGATATAAAAATAAGCTTACGACAAATGGGACAAGTGGCTCAAGTCAAATCAATCCCTAACTTTATCGAAC
AATATAAAAATCAGGACGTAAAGAAACTCAGGATAGACTATAAAAAATTTTCCTCCTTTTCCATTCTTGATGACCTTTAT
CCCGAGCGTCTAAGAGAAATCTACAACCCACCCGTTTTAATTTTTTATCAGGGAAATATTGATTTATTAAAAAATCCTAA
ACTTGCCTTTGTTGGAAGCCGCCTAGCAGGTCAATCAGGGATAAAAGCTGTCCAAAAAATTATCACAGAATTGAATCAAT
CCTTTACAATAGTAAGCGGGCTTGCTAAAGGGATTGACACAGCCAGCCATCTGTCAGCAATTAAAACTAAAACACCAACA
ATTGCTGTTATTGGTACAGGATTGGATATTTTCTATCCACTAGAAAATCGTAAAATACAGGAATATCTAGCAAAAAATCA
ATTAGTTTTAAGTGAATATTCATTAGGCGAAAAACCACTTAAATACCACTTTCCAGAGCGCAATCGAATTATTGCTGGTC
TTTCACGGGGAGTTGTTGTCGTGGAAGCAAAATTAAGAAGTGGCAGTTTAATCACCTGTGAAAGGGCACTGGAAGAAGGA
AGAGATATCTTTGCCATCCCTGGAAATATTGCAGATGGAACATCTGATGGCTGTAATCATCTTATTCAACAAGGAGCAAA
ACTTGTCTATCAAGCTCAAGATATTTTAGAAGAATATTTGTACAATTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(69-270)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(2-63)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Lactococcus lactis subsp. cremoris KW2

97.872

100

0.979
  dprA Streptococcus mutans UA159

59.353

98.582

0.585
  dprA/cilB/dalA Streptococcus mitis SK321

56.071

99.291

0.557
  dprA/cilB/dalA Streptococcus pneumoniae R6

55.714

99.291

0.553
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

55.714

99.291

0.553
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

55.714

99.291

0.553
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

55.714

99.291

0.553
  dprA/cilB/dalA Streptococcus pneumoniae D39

55.714

99.291

0.553
  dprA Vibrio campbellii strain DS40M4

35.644

100

0.383
  dprA Bacillus subtilis subsp. subtilis str. 168

39.273

97.518

0.383
  dprA Legionella pneumophila strain ERS1305867

39.13

97.872

0.383
  dprA Haemophilus influenzae Rd KW20

39.63

95.745

0.379

Multiple sequence alignment