Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LGV81_RS02510 Genome accession   NZ_CP086265
Coordinates   509970..510479 (+) Length   169 a.a.
NCBI ID   WP_020831292.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RS647     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504970..515479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGV81_RS02485 (LGV81_02495) - 505316..505942 (+) 627 WP_011000512.1 DUF2889 domain-containing protein -
  LGV81_RS02490 (LGV81_02500) sucC 506009..507175 (+) 1167 WP_011000513.1 ADP-forming succinate--CoA ligase subunit beta -
  LGV81_RS02495 (LGV81_02505) sucD 507260..508141 (+) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  LGV81_RS02500 (LGV81_02510) - 508291..508989 (+) 699 WP_020831289.1 TerC family protein -
  LGV81_RS02505 (LGV81_02515) pilA 509183..509704 (+) 522 WP_020831290.1 pilin Machinery gene
  LGV81_RS02510 (LGV81_02520) pilA 509970..510479 (+) 510 WP_020831292.1 pilin Machinery gene
  LGV81_RS02515 (LGV81_02525) - 510556..512289 (+) 1734 WP_028852633.1 PglL family O-oligosaccharyltransferase -
  LGV81_RS02520 (LGV81_02530) moaC 512518..512994 (-) 477 WP_011000519.1 cyclic pyranopterin monophosphate synthase MoaC -
  LGV81_RS02525 (LGV81_02535) - 513088..514776 (+) 1689 WP_020831294.1 M48 family metallopeptidase -
  LGV81_RS02530 (LGV81_02540) - 514791..515378 (-) 588 WP_020831295.1 DUF2946 family protein -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 17122.83 Da        Isoelectric Point: 9.9491

>NTDB_id=541676 LGV81_RS02510 WP_020831292.1 509970..510479(+) (pilA) [Ralstonia pseudosolanacearum strain RS647]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAANAQTDLSVGSSVFT
PTKNVAGLAIDGTTTGVPGQITITYTQAAGGGTLALVPTAAGAKLPTSSAPSGPIMWTCYAAGKAQAASSVAPNATMSLA
AKYAPAECR

Nucleotide


Download         Length: 510 bp        

>NTDB_id=541676 LGV81_RS02510 WP_020831292.1 509970..510479(+) (pilA) [Ralstonia pseudosolanacearum strain RS647]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACGGTTCGTGCACGCGTGACGGAAGGTCTGTCGCTGG
CTGCACAAGCCAAGGCACTGGTGTCGGAAAACGCTGCCAATGCACAGACTGACCTGTCGGTGGGTTCGTCGGTGTTCACG
CCGACCAAGAACGTTGCAGGCCTTGCAATTGACGGTACTACGACTGGCGTTCCGGGCCAGATCACCATCACCTATACGCA
GGCTGCCGGCGGCGGCACGCTGGCTCTGGTCCCGACCGCGGCAGGCGCTAAGCTGCCGACGAGTTCGGCTCCGTCGGGTC
CGATCATGTGGACCTGCTACGCTGCGGGTAAGGCACAGGCTGCCAGCAGCGTTGCTCCGAACGCTACGATGTCGCTGGCA
GCGAAGTATGCTCCGGCAGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(43-169)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

91.124

100

0.911
  pilA2 Legionella pneumophila str. Paris

51.235

95.858

0.491
  pilA2 Legionella pneumophila strain ERS1305867

50.617

95.858

0.485
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.462

100

0.444
  comP Acinetobacter baylyi ADP1

43.023

100

0.438
  pilA/pilA1 Eikenella corrodens VA1

38.506

100

0.396