Detailed information NTG0539591

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: JRY11_RS04225
• Genome accession: NZ_CP070382
• Coordinates: 818168..819241 (+)
• Length: 357 a.a.
• NCBI ID: WP_074453836.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain KF140
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 813168..824241

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JRY11_RS04220 (JRY11_000852)	comGA	817336..818274 (+)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene
JRY11_RS04225 (JRY11_000853)	comGB	818168..819241 (+)	1074	WP_074453836.1	competence type IV pilus assembly protein ComGB	Machinery gene
JRY11_RS04230 (JRY11_000854)	comGC	819369..819638 (+)	270	WP_023188581.1	competence type IV pilus major pilin ComGC	Machinery gene
JRY11_RS04235 (JRY11_000855)	comGD	819670..820029 (+)	360	WP_023188582.1	competence type IV pilus minor pilin ComGD	Machinery gene
JRY11_RS04240 (JRY11_000856)	comGE	820001..820297 (+)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
JRY11_RS04245 (JRY11_000857)	comGF	820260..820706 (+)	447	WP_029344525.1	competence type IV pilus minor pilin ComGF	Machinery gene
JRY11_RS04250 (JRY11_000858)	comGG	820745..821029 (+)	285	WP_025017139.1	competence type IV pilus minor pilin ComGG	Machinery gene
JRY11_RS04255 (JRY11_000859)	-	821127..821564 (+)	438	WP_003129992.1	zinc-dependent MarR family transcriptional regulator	-
JRY11_RS04260 (JRY11_000860)	-	821561..822403 (+)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
JRY11_RS04265 (JRY11_000861)	-	822580..823317 (+)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
JRY11_RS04270 (JRY11_000862)	-	823310..824119 (+)	810	WP_014570791.1	metal ABC transporter permease	-

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 40340.46 Da
• Isoelectric Point: 10.1966

>NTDB_id=539591 JRY11_RS04225 WP_074453836.1 818168..819241(+) (comGB) [Lactococcus lactis strain KF140]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLEVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide

• Length: 1074 bp

>NTDB_id=539591 JRY11_RS04225 WP_074453836.1 818168..819241(+) (comGB) [Lactococcus lactis strain KF140]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTGAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (227-348)

• Type II secretion system (T2SS), protein F (T2SSF) (41-159)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	82.991	95.518	0.793
comYB	Streptococcus gordonii str. Challis substr. CH1	53.666	95.518	0.513
comYB	Streptococcus mutans UA140	53.666	95.518	0.513
comYB	Streptococcus mutans UA159	53.372	95.518	0.51
comGB/cglB	Streptococcus mitis SK321	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae TIGR4	50.746	93.838	0.476
comGB/cglB	Streptococcus pneumoniae Rx1	50.746	93.838	0.476
comGB/cglB	Streptococcus pneumoniae D39	50.746	93.838	0.476
comGB/cglB	Streptococcus pneumoniae R6	50.746	93.838	0.476
comGB/cglB	Streptococcus mitis NCTC 12261	50.149	93.838	0.471