Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C380_RS04540 Genome accession   NC_018708
Coordinates   1007661..1009400 (-) Length   579 a.a.
NCBI ID   WP_015012716.1    Uniprot ID   K0HXW9
Organism   Acidovorax sp. KKS102     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1002661..1014400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C380_RS04510 (C380_04550) - 1003168..1003638 (+) 471 WP_015012710.1 NUDIX domain-containing protein -
  C380_RS04515 (C380_04555) - 1003756..1003974 (-) 219 WP_015012711.1 DNA gyrase inhibitor YacG -
  C380_RS04520 (C380_04560) zapD 1004077..1004832 (-) 756 WP_015012712.1 cell division protein ZapD -
  C380_RS04525 (C380_04565) coaE 1004918..1005538 (-) 621 WP_015012713.1 dephospho-CoA kinase -
  C380_RS04530 (C380_04570) - 1005548..1006420 (-) 873 WP_015012714.1 A24 family peptidase -
  C380_RS04535 (C380_04575) pilC 1006420..1007640 (-) 1221 WP_015012715.1 type II secretion system F family protein Machinery gene
  C380_RS04540 (C380_04580) pilB 1007661..1009400 (-) 1740 WP_015012716.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C380_RS04550 (C380_04585) - 1009650..1010579 (-) 930 WP_015012717.1 polyprenyl synthetase family protein -
  C380_RS04555 (C380_04590) rplU 1010814..1011125 (+) 312 WP_015012718.1 50S ribosomal protein L21 -
  C380_RS04560 (C380_04595) rpmA 1011141..1011398 (+) 258 WP_005794180.1 50S ribosomal protein L27 -
  C380_RS04565 (C380_04600) cgtA 1011569..1012639 (+) 1071 WP_015012719.1 Obg family GTPase CgtA -
  C380_RS04570 (C380_04605) proB 1012729..1013871 (+) 1143 WP_015012720.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63089.51 Da        Isoelectric Point: 5.4629

>NTDB_id=53893 C380_RS04540 WP_015012716.1 1007661..1009400(-) (pilB) [Acidovorax sp. KKS102]
MAAVDTALKEASAVALPGLGRALMSAGKLTQKSAEDIYKKSQISRTSFIAELTGSGAVSAADLAHTVSAVFGAPLLDLDA
IDPLRLPKELLDNKICQAYRVVVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLNRLIEATTKSASEAM
DTLVSGGGDFAFDDFAVEDAPEEQDTGATEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASP
PIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPIEK
ERLLQAISRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQD
PDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPVCKVPADI
PHEALVDAGYEEHEIDGSWVTYRPVGCSACNNGYKGRLGIYQVMPVSEEIQRIILRDGSALEIAKQARAEGVRSLRESGL
YKAKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=53893 C380_RS04540 WP_015012716.1 1007661..1009400(-) (pilB) [Acidovorax sp. KKS102]
ATGGCCGCTGTTGATACTGCCCTCAAAGAAGCTTCCGCAGTCGCTCTGCCGGGGCTTGGCCGTGCCCTGATGTCTGCCGG
CAAACTGACCCAGAAGTCAGCGGAAGACATTTACAAAAAATCACAAATCAGCCGAACCAGTTTCATTGCGGAACTTACGG
GTTCGGGCGCTGTTTCTGCAGCGGATCTCGCGCATACGGTATCAGCGGTCTTTGGCGCACCCTTGCTCGATCTGGATGCC
ATTGATCCATTGCGTCTGCCAAAGGAACTGCTAGATAACAAGATATGCCAAGCATATCGCGTAGTGGTTCTCAGCAAACG
AAATAATCGACTGATCGTAGCAACGGCCGACCCAACCGATCAAGAAGCTGCAGAGAAAATAAAATTCACTACCCAAATGG
GTGTGGATTGGATCATTGCTGAATATGACAAGCTTAACCGCCTGATAGAAGCCACCACAAAATCAGCAAGCGAGGCAATG
GATACGCTCGTTAGTGGGGGCGGAGACTTCGCGTTTGATGACTTTGCCGTCGAGGATGCACCCGAGGAGCAGGATACAGG
CGCAACAGAAGTTGAAGATGCCCCGATTGTCAAGTTTCTACACAAAATGTTGCTCGATGCATTCAATATGCGTGCATCGG
ATTTGCATTTCGAGCCTTACGAGCACCAGTACCGCGTTCGGTTTCGCATTGACGGGGAACTGCGTGAGATAGCCTCGCCG
CCGATCGCCATCAAAGACAAACTCGCGTCCCGTATAAAGGTAATTTCGCGCTTGGATATTTCGGAAAAGCGCGTTCCCCA
AGATGGTCGCATGAAGCTCAAAGTGGGGCCAGATCGAGTGATCGATTTTCGGGTAAGTACCTTGCCTACCCTGTTCGGCG
AAAAAATTGTTATTCGCATTTTGGATCCGAGCAGTGCGAAACTCGGTATCGATGCTCTCGGGTATGAGCCCATCGAGAAG
GAGCGATTACTTCAGGCCATCAGTCGTCCCTACGGAATGATTTTAGTCACAGGTCCGACAGGCTCCGGCAAAACGGTCTC
TCTCTACACCTGTTTGAACCTGTTGAATAAGCCCGGTGTCAATATCGCAACAGCCGAAGATCCTTCTGAAATCAATCTGC
CTGGCGTTAACCAAGTCAATGTAAATGAAAAGGCCGGACTGACCTTTGCAGTGGCGTTGAAGTCATTTCTTCGCCAAGAT
CCCGACATCATCATGGTGGGTGAAATTCGTGATCTAGAGACCGCAGATATTTCTATTAAAGCAGCGCAGACGGGTCATCT
TGTGCTTTCTACTCTGCACACAAATGACGCTCCCACCACGCTTACACGGATGCGCAACATGGGTATAGCGCCATTCAATA
TCGCATCCAGTGTGATTCTGATTACTGCGCAGCGGCTGGCCCGTCGGTTGTGCCCCGTGTGCAAGGTTCCCGCCGACATC
CCGCATGAGGCTCTAGTGGACGCTGGATACGAAGAACACGAGATAGATGGTTCTTGGGTTACTTATCGCCCTGTAGGTTG
CTCGGCCTGTAACAATGGCTACAAAGGGCGACTCGGCATCTATCAGGTGATGCCTGTTTCCGAAGAGATCCAACGCATCA
TCCTCCGTGATGGGAGTGCTTTAGAAATCGCGAAGCAGGCTCGTGCGGAGGGTGTTCGGTCGCTGCGTGAATCGGGGCTC
TACAAGGCCAAATTAGGCCTCACTTCGCTGGAAGAAGTGCTGGCTGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K0HXW9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.966

100

0.525
  pilB Acinetobacter baylyi ADP1

53.158

98.446

0.523
  pilF Neisseria gonorrhoeae MS11

51.858

97.582

0.506
  pilB Legionella pneumophila strain ERS1305867

49.468

97.409

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

45.009

98.618

0.444
  pilB Vibrio campbellii strain DS40M4

45.567

97.409

0.444
  pilB Vibrio cholerae strain A1552

47.692

89.81

0.428
  pilF Thermus thermophilus HB27

40.204

100

0.408
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.986

98.791

0.385

Multiple sequence alignment