Detailed information NTG0538893

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLUL021_RS09175
• Genome accession: NZ_CP070263
• Coordinates: 1852697..1853344 (-)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis subsp. lactis strain UL021
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1847697..1858344

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUL021_RS09150 (LLUL021_09130)	atpF	1847767..1848273 (-)	507	WP_004255258.1	F0F1 ATP synthase subunit B	-
LLUL021_RS09155 (LLUL021_09135)	atpB	1848288..1849001 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLUL021_RS09160 (LLUL021_09140)	-	1849046..1849261 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLUL021_RS09165 (LLUL021_09145)	-	1849445..1850221 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LLUL021_RS09170 (LLUL021_09150)	comEC	1850506..1852716 (-)	2211	WP_137016458.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LLUL021_RS09175 (LLUL021_09155)	comEA	1852697..1853344 (-)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
LLUL021_RS09180 (LLUL021_09160)	-	1853404..1854765 (-)	1362	WP_021214597.1	ABC transporter permease	-
LLUL021_RS09185 (LLUL021_09165)	-	1854762..1855694 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLUL021_RS09190 (LLUL021_09170)	-	1855799..1856197 (-)	399	WP_004255236.1	hypothetical protein	-
LLUL021_RS09195 (LLUL021_09175)	-	1856301..1856864 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LLUL021_RS09200 (LLUL021_09180)	-	1857037..1858215 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=538893 LLUL021_RS09175 WP_004255240.1 1852697..1853344(-) (comEA) [Lactococcus lactis subsp. lactis strain UL021]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=538893 LLUL021_RS09175 WP_004255240.1 1852697..1853344(-) (comEA) [Lactococcus lactis subsp. lactis strain UL021]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367