Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AMBAS45_RS15080 Genome accession   NC_018679
Coordinates   3492852..3493964 (-) Length   370 a.a.
NCBI ID   WP_014977241.1    Uniprot ID   A0AB33A1W2
Organism   Alteromonas macleodii str. 'Balearic Sea AD45'     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3487852..3498964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMBAS45_RS15055 (AMBAS45_14855) - 3489408..3490136 (+) 729 WP_014980069.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AMBAS45_RS15060 (AMBAS45_14860) gshB 3490275..3491228 (+) 954 WP_014980070.1 glutathione synthase -
  AMBAS45_RS15065 (AMBAS45_14865) - 3491357..3491923 (+) 567 WP_014950460.1 YqgE/AlgH family protein -
  AMBAS45_RS15070 (AMBAS45_14870) ruvX 3491916..3492347 (+) 432 WP_014980071.1 Holliday junction resolvase RuvX -
  AMBAS45_RS15075 (AMBAS45_14875) - 3492394..3492711 (-) 318 WP_014980072.1 DUF2007 domain-containing protein -
  AMBAS45_RS15080 (AMBAS45_14880) pilU 3492852..3493964 (-) 1113 WP_014977241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMBAS45_RS15085 (AMBAS45_14885) pilT 3493973..3495016 (-) 1044 WP_014950464.1 type IV pilus twitching motility protein PilT Machinery gene
  AMBAS45_RS15090 (AMBAS45_14890) - 3495044..3495730 (+) 687 WP_014980073.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMBAS45_RS15095 (AMBAS45_14895) proC 3495801..3496622 (+) 822 WP_014980074.1 pyrroline-5-carboxylate reductase -
  AMBAS45_RS15100 (AMBAS45_14900) - 3496643..3497179 (+) 537 WP_014980075.1 YggT family protein -
  AMBAS45_RS15105 (AMBAS45_14905) - 3497276..3497749 (+) 474 WP_014980076.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40781.69 Da        Isoelectric Point: 6.0487

>NTDB_id=53714 AMBAS45_RS15080 WP_014977241.1 3492852..3493964(-) (pilU) [Alteromonas macleodii str. 'Balearic Sea AD45']
MLDSLLQKMVDTNASDLFVTAGFPVSAKINGKLTPVTESATSEEASLALVHEAMTEKQQNEFHTTKECNFAIVREGIGRF
RCSAFWQRDQAGMVIRRIVTQIPKADDLGLPRVLKEIIMAKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGKIDMDQALHHADSPNDLRLMIKLDTSDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=53714 AMBAS45_RS15080 WP_014977241.1 3492852..3493964(-) (pilU) [Alteromonas macleodii str. 'Balearic Sea AD45']
ATGTTAGATTCACTACTACAAAAAATGGTCGATACCAACGCTTCGGATTTATTCGTAACGGCGGGGTTTCCGGTAAGTGC
CAAAATTAACGGCAAACTTACGCCAGTGACGGAAAGCGCGACCTCGGAAGAGGCATCGCTGGCGCTGGTTCACGAAGCGA
TGACTGAAAAACAGCAGAATGAGTTTCACACGACCAAAGAATGTAATTTCGCCATAGTACGAGAGGGCATTGGTCGTTTC
CGCTGCTCTGCATTTTGGCAGCGTGACCAAGCGGGCATGGTGATTCGCCGCATCGTTACCCAAATTCCGAAAGCTGATGA
TTTGGGTCTGCCGCGAGTATTAAAAGAAATTATTATGGCTAAACGCGGTCTTGTTCTATTTGTAGGTGGAACAGGCACGG
GTAAATCAACGTCATTGGCTGCGCTTATTGGGCATAGAAATGAAAATTCGCACGGGCATATACTGACTATTGAAGACCCC
ATTGAATTTGTGCACGAGCATAAAAACTGTGTTATTACCCAGCGCGAGGTGGGCATAGATACTCAGTCTTTCGACGATGC
GCTTAAAAGTTCACTACGTCAGGCGCCTGATGTCATTCTTATTGGTGAAATTCGTTCAATGGAAACCATGGAATATGCCA
TGTCGTTTGCTGATACAGGGCATTTGTGTGTGGCTACGCTGCACGCTAACAACGCGAACCAAGCTATTGAACGTATTATG
CATTTAGCGCCGAAAGATCAGCACGATAAGTTGCGCTTTGACTTAAGCTTAAACATCCGCGCCATTGTTGCGCAGCAACT
AGTACCAACGCCAGACGGCAAGGGTAGGGTTGCGGCAATAGAAATACTGCTAAACTCGCCGCTAGTGCGCGACCTAATAC
AGCGCAATGAAATAGGCGCGTTAAAAGAAGCAATGAAAAAAGGCAAAGAGCAGGGCATGCAGAGCTTTGATATGGCGCTG
TATGATTTGTACAAAGCGGGTAAGATTGATATGGACCAAGCGCTGCACCACGCCGACTCGCCCAACGATTTGCGCTTGAT
GATCAAGCTAGATACCAGCGACGGTTCTAGCTTAGGTACACTGTCAAATGTATCTATTGATATGGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Acinetobacter baylyi ADP1

59.143

94.595

0.559
  pilU Vibrio cholerae strain A1552

56.25

99.459

0.559
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.647

91.892

0.392
  pilT Acinetobacter nosocomialis M2

39.412

91.892

0.362
  pilT Acinetobacter baumannii D1279779

39.412

91.892

0.362
  pilT Acinetobacter baumannii strain A118

39.412

91.892

0.362

Multiple sequence alignment