Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   JQU52_RS12625 Genome accession   NZ_CP069798
Coordinates   2471431..2471886 (-) Length   151 a.a.
NCBI ID   WP_328300589.1    Uniprot ID   A0A892ZGD6
Organism   Paralysiella testudinis strain 26B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2466431..2476886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JQU52_RS12590 (JQU52_12590) - 2466675..2467009 (-) 335 Protein_2468 transposase -
  JQU52_RS12595 (JQU52_12600) - 2467030..2467242 (-) 213 WP_230338818.1 hypothetical protein -
  JQU52_RS12600 (JQU52_12605) - 2467226..2467678 (-) 453 WP_230338819.1 transposase -
  JQU52_RS12605 (JQU52_12610) - 2468672..2469658 (-) 987 WP_230338820.1 nucleoside hydrolase -
  JQU52_RS12610 (JQU52_12615) tfpZ 2470053..2470787 (-) 735 WP_328300585.1 TfpX/TfpZ family type IV pilin accessory protein -
  JQU52_RS12615 (JQU52_12620) - 2470877..2471356 (-) 480 WP_328300587.1 pilin -
  JQU52_RS12625 (JQU52_12625) pilA/pilA1 2471431..2471886 (-) 456 WP_328300589.1 pilin Machinery gene
  JQU52_RS12635 (JQU52_12630) - 2472434..2474710 (-) 2277 WP_230338822.1 NADP-dependent malic enzyme -
  JQU52_RS12640 (JQU52_12635) - 2474843..2475427 (-) 585 WP_268866617.1 FixH family protein -
  JQU52_RS12645 (JQU52_12640) ccoG 2475417..2476832 (-) 1416 WP_230338823.1 cytochrome c oxidase accessory protein CcoG -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15371.73 Da        Isoelectric Point: 9.0147

>NTDB_id=536761 JQU52_RS12625 WP_328300589.1 2471431..2471886(-) (pilA/pilA1) [Paralysiella testudinis strain 26B]
MKALQKGFTLIELMIVIAIIGILAAIALPAYQDYTARAQATEGFKATSGLQTDIGTYAADKGSLKDVGKVGGVIDLQAKA
LEGKYFAAKSVSVGADDGVITISFTSGANSGKSMTLSPNLVAASGQISKWTCAPAAGANGIEAKRLPTSCQ

Nucleotide


Download         Length: 456 bp        

>NTDB_id=536761 JQU52_RS12625 WP_328300589.1 2471431..2471886(-) (pilA/pilA1) [Paralysiella testudinis strain 26B]
ATGAAAGCATTGCAAAAAGGTTTTACCCTGATTGAATTGATGATTGTAATCGCGATTATCGGTATTTTGGCTGCCATCGC
TCTGCCGGCCTACCAAGACTACACCGCCCGTGCGCAAGCTACTGAAGGCTTTAAGGCTACTTCTGGTTTGCAAACCGATA
TTGGTACCTATGCCGCAGATAAAGGAAGTTTAAAAGATGTTGGTAAAGTTGGTGGTGTTATCGATCTCCAAGCTAAAGCT
CTGGAAGGTAAATATTTTGCTGCGAAGTCGGTAAGTGTTGGCGCAGATGACGGTGTAATTACAATTTCATTTACCTCAGG
CGCTAATAGCGGCAAATCCATGACGCTTAGTCCAAACTTAGTAGCCGCGAGTGGCCAAATATCAAAATGGACATGTGCGC
CGGCTGCTGGTGCCAATGGTATTGAGGCGAAACGTTTGCCTACTTCATGCCAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)

Pilin (bacterial filament) (Pilin)

(36-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

67.974

100

0.689
  pilE Neisseria gonorrhoeae MS11

40.12

100

0.444
  pilE Neisseria gonorrhoeae strain FA1090

39.394

100

0.43
  pilA Ralstonia pseudosolanacearum GMI1000

38.235

100

0.43
  pilA2 Legionella pneumophila strain ERS1305867

41.497

97.351

0.404
  pilA2 Legionella pneumophila str. Paris

41.497

97.351

0.404
  comP Acinetobacter baylyi ADP1

36.538

100

0.377