Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D1098_RS02930 Genome accession   NZ_CP069797
Coordinates   624491..625885 (+) Length   464 a.a.
NCBI ID   WP_005615186.1    Uniprot ID   A3N0N9
Organism   Actinobacillus pleuropneumoniae strain K17     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 619491..630885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1098_RS02900 (D1098_02925) - 619607..620383 (-) 777 WP_005597402.1 TSUP family transporter -
  D1098_RS02905 (D1098_02930) proB 620396..621487 (-) 1092 WP_005601213.1 glutamate 5-kinase -
  D1098_RS02910 (D1098_02935) folA 621574..622062 (+) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1098_RS02915 (D1098_02940) - 622189..622422 (+) 234 WP_005597399.1 YgjV family protein -
  D1098_RS02920 (D1098_02945) radA 622466..623845 (-) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1098_RS02925 (D1098_02950) pilA 624027..624473 (+) 447 WP_009874618.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1098_RS02930 (D1098_02955) pilB 624491..625885 (+) 1395 WP_005615186.1 GspE/PulE family protein Machinery gene
  D1098_RS02935 (D1098_02960) pilC 625869..627071 (+) 1203 WP_005601207.1 type II secretion system F family protein Machinery gene
  D1098_RS02940 (D1098_02965) - 627098..627736 (+) 639 WP_009874617.1 prepilin peptidase -
  D1098_RS02945 (D1098_02970) coaE 627758..628396 (+) 639 WP_009874616.1 dephospho-CoA kinase -
  D1098_RS02950 (D1098_02975) yacG 628378..628566 (+) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1098_RS02955 (D1098_02980) holA 628643..629668 (-) 1026 WP_009874615.1 DNA polymerase III subunit delta -
  D1098_RS02960 (D1098_02985) lptE 629777..630271 (-) 495 WP_005597385.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 53026.89 Da        Isoelectric Point: 6.5042

>NTDB_id=536718 D1098_RS02930 WP_005615186.1 624491..625885(+) (pilB) [Actinobacillus pleuropneumoniae strain K17]
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHFEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKAGITSLEEIERVLGHVESV

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=536718 D1098_RS02930 WP_005615186.1 624491..625885(+) (pilB) [Actinobacillus pleuropneumoniae strain K17]
ATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTCCGGATTTCCGAGGAACAGTGGCAAAAAAATTATGATGA
ACGTCATATCTTATTACGTTATCTCACCGTACCGCTTCGGGAAGACCAACAAACATTATGGTTGGCAATCGATGATATGA
ATAATCTCAGTGCTTGCGAGATTTTTGCTTTTCTTACGCATAAGCGAATTGAGCCGGTATTGGTCTCTTCCGAAGAATTG
AAATACCTGCTCGGGCAATTAATGCCGGAACAACAATCGGTATATGACGAAACGGAATTACCTTATCAACCTTTAGCACA
AGCGCAGCATTCGGATGTTAATGATCCGATTATTCAAGTGTTGGACGGCCTGTTTAAATACGGTTTAAAACATAATGCTT
CGGATATTCATTTTGAACCGCAAAAAGACAAAATGTTGATTCGATTACGTATCGACGGCGTGTTACATCCGTATAAATCA
TTTTCTCTCCAACTTGCCAATCGACTTATTTCTCGCATTAAATTATTAGCTAAACTGGATATTAGTGAATCCCGTCTTCC
GCAAGACGGGCAATTCGGTTTTAAGACAATCTTATCCGAAACATTGGATTTCCGTGTTTCGAGTTTACCGACTTATTGGG
GCGAAAAAGTCGTTTTACGGCTACAAAAAAATAAACCGACGCATTTTGATTTTACTACGTTGGGCTTCTTGGCGGAGCAG
AAGGAAAAGTTGTTGCGGGCATTGCGGCAGCCTCAAGGATTAATATTAGTTACGGGACCGACCGGCAGTGGTAAAAGTAT
TACCTTATATAGTGCGTTGGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCCGAAGATCCGATAGAAATCGAAC
TTGAAGGCATAATCCAAACGCAAATAAACCCAGCGATAGATTTAGGGTTCAGCCGGCTATTAAGAACGTTTTTACGACAA
GATCCGGATGTGATTATGTTAGGTGAAATTCGTGATCAAGAAAGTGCCGAAATTGCATTAAGTGCGGCACAAACCGGGCA
TTTAGTGCTTTCGACCTTACATACCAATGATGCGCCTTCGGCAATCGAACGCTTATTGCAGCTCGGTATCAAAGAATATG
AAATCAATAATTCGTTATTACTTGTCGTGGCGCAACGTTTAGTTCGTAAATTATGTACCGAATGCGGCGGAAAAGGCTGT
AAATATTGTTATCACGGCTACAGAGGCAGAATCGGCGTTTATCAACTGTTTTCAAAAAATGGAAAAATTTTTGAGAAATC
GACCGCTTGTTTGGATTTTGAAAACTTATATCAAAGTGCGCAACAGAAATTCAAGGCGGGAATCACTTCATTAGAAGAAA
TCGAGAGAGTGTTAGGTCATGTTGAAAGTGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3N0N9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

68.192

98.922

0.675
  pilB Haemophilus influenzae Rd KW20

58.499

97.629

0.571
  pilB Haemophilus influenzae 86-028NP

58.057

97.629

0.567
  pilB Vibrio campbellii strain DS40M4

40.333

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.416
  pilB Vibrio parahaemolyticus RIMD 2210633

39.917

100

0.414
  pilB Acinetobacter baumannii D1279779

39.059

100

0.412
  pilB Vibrio cholerae strain A1552

39.212

100

0.407
  pilB Acinetobacter baylyi ADP1

38.525

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.538

100

0.369