Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   D1098_RS02925 Genome accession   NZ_CP069797
Coordinates   624027..624473 (+) Length   148 a.a.
NCBI ID   WP_009874618.1    Uniprot ID   A3N0P0
Organism   Actinobacillus pleuropneumoniae strain K17     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 619027..629473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1098_RS02900 (D1098_02925) - 619607..620383 (-) 777 WP_005597402.1 TSUP family transporter -
  D1098_RS02905 (D1098_02930) proB 620396..621487 (-) 1092 WP_005601213.1 glutamate 5-kinase -
  D1098_RS02910 (D1098_02935) folA 621574..622062 (+) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1098_RS02915 (D1098_02940) - 622189..622422 (+) 234 WP_005597399.1 YgjV family protein -
  D1098_RS02920 (D1098_02945) radA 622466..623845 (-) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1098_RS02925 (D1098_02950) pilA 624027..624473 (+) 447 WP_009874618.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1098_RS02930 (D1098_02955) pilB 624491..625885 (+) 1395 WP_005615186.1 GspE/PulE family protein Machinery gene
  D1098_RS02935 (D1098_02960) pilC 625869..627071 (+) 1203 WP_005601207.1 type II secretion system F family protein Machinery gene
  D1098_RS02940 (D1098_02965) - 627098..627736 (+) 639 WP_009874617.1 prepilin peptidase -
  D1098_RS02945 (D1098_02970) coaE 627758..628396 (+) 639 WP_009874616.1 dephospho-CoA kinase -
  D1098_RS02950 (D1098_02975) yacG 628378..628566 (+) 189 WP_005597388.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16006.46 Da        Isoelectric Point: 7.6257

>NTDB_id=536717 D1098_RS02925 WP_009874618.1 624027..624473(+) (pilA) [Actinobacillus pleuropneumoniae strain K17]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCAIN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=536717 D1098_RS02925 WP_009874618.1 624027..624473(+) (pilA) [Actinobacillus pleuropneumoniae strain K17]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACGGTAACGGGGAA
GGGGAATCTGCAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGTGCGATAAATTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3N0P0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.172

97.973

0.541
  pilA Haemophilus influenzae Rd KW20

53.103

97.973

0.52