Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6J46_RS06270 Genome accession   NZ_CP069586
Coordinates   1314496..1316208 (-) Length   570 a.a.
NCBI ID   WP_204085778.1    Uniprot ID   -
Organism   Acinetobacter pittii strain FDAARGOS_1216     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1309496..1321208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J46_RS06240 (I6J46_06240) - 1309659..1309985 (+) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -
  I6J46_RS06245 (I6J46_06245) rlmB 1310088..1310837 (+) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I6J46_RS06250 (I6J46_06250) - 1310873..1311787 (+) 915 WP_039763523.1 DMT family transporter -
  I6J46_RS06255 (I6J46_06255) coaE 1311784..1312380 (-) 597 WP_039763520.1 dephospho-CoA kinase -
  I6J46_RS06260 (I6J46_06260) pilD 1312382..1313242 (-) 861 WP_002114758.1 prepilin peptidase Machinery gene
  I6J46_RS06265 (I6J46_06265) pilC 1313242..1314468 (-) 1227 WP_005079343.1 type II secretion system F family protein Machinery gene
  I6J46_RS06270 (I6J46_06270) pilB 1314496..1316208 (-) 1713 WP_204085778.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6J46_RS06275 (I6J46_06275) tpiA 1316500..1317294 (+) 795 WP_016142374.1 triose-phosphate isomerase -
  I6J46_RS06280 (I6J46_06280) secG 1317307..1317636 (+) 330 WP_016142373.1 preprotein translocase subunit SecG -
  I6J46_RS06300 (I6J46_06300) rimP 1318274..1318798 (+) 525 WP_002114922.1 ribosome maturation factor RimP -
  I6J46_RS06305 (I6J46_06305) nusA 1318836..1320320 (+) 1485 WP_204085779.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63611.02 Da        Isoelectric Point: 5.0932

>NTDB_id=535783 I6J46_RS06270 WP_204085778.1 1314496..1316208(-) (pilB) [Acinetobacter pittii strain FDAARGOS_1216]
MSVIHTSPKFSGFFRRLVEEKHVSATTMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILSQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPADIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=535783 I6J46_RS06270 WP_204085778.1 1314496..1316208(-) (pilB) [Acinetobacter pittii strain FDAARGOS_1216]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAACGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACCAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGATTTTAAAACATCGGGTCTTACCTCTTATACAAAGAGGGCAGATTTTATATGT
CGCGACCAGCAATCCAAGTAATATTGAAGCGATCGATGCTATTCGCTTTAATAGCAAGCTCATGGTTGAGCCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGTCGCAGCAATTTACCGAAGAAAGCAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACACCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGAGCTTCAGACTTGCATTTTGAACCCTATGAAAAATCAT
ATCGGGTCCGTTATCGAGTCGATGGTGTATTGCGTCAGATTGCAAATCCTCCTTTGCAATTGGCTAACCGTTTAGCTTCA
CGCTTAAAAGTCATGTCCCAAATGGATATTTCCGAAAAGCGAGTCCCTCAAGATGGTCGTATTAAGCTCAAGCTATCGAA
ATCTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACCTTATTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTGGATAAGCCA
CAAGGTATGCTTCTAATTACAGGCCCTACAGGTTCCGGTAAAACGGTTTCTTTATATACGGGCTTAAATATTTTAAATAC
TGAAAGTTCGAATATTTCCACCGCCGAAGATCCAGTTGAAATTAACCTTGAAGGAATTAATCAGGTTAACGTCAACCCAA
AAGTAGGTCTGACTTTTTCTGCTGCACTTAAATCATTTTTACGTCAAGATCCGGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTCATGTCAACACTGCATACCAATAGTGC
TCCAGAAACGCTAACTCGCCTACGCAATATGGGTGTTCCATCCTTTAATATCGCAACATCAGTCAACTTGGTTATTGCAC
AGCGTTTGGCACGTCGCTTATGCCCTCAGTGTAAAATACCTGCTGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATCTGACTCATCCCGATTTTCAAGTTTTCCAGCCTGTCGGTTGTACTGAGTGTCGTGAAGGCTATAAAGG
TCGAGTAGGTATTTACGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTTACTTCT
TTACAGGAAGTCAATCGCGTGACTAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

95.439

100

0.954
  pilB Acinetobacter baylyi ADP1

78.596

100

0.786
  pilB Legionella pneumophila strain ERS1305867

52.028

99.474

0.518
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.509

92.982

0.479
  pilB Vibrio campbellii strain DS40M4

50.958

91.579

0.467
  pilB Vibrio cholerae strain A1552

50.674

91.053

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384
  pilF Thermus thermophilus HB27

36.655

98.596

0.361