Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LG939_RS17970 Genome accession   NZ_CP084738
Coordinates   3830903..3831412 (-) Length   169 a.a.
NCBI ID   WP_020831292.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RS639     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3825903..3836412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG939_RS17950 (LG939_17955) - 3826004..3826591 (+) 588 WP_377269722.1 DUF2946 family protein -
  LG939_RS17955 (LG939_17960) - 3826606..3828294 (-) 1689 WP_020831294.1 M48 family metallopeptidase -
  LG939_RS17960 (LG939_17965) moaC 3828388..3828864 (+) 477 WP_011000519.1 cyclic pyranopterin monophosphate synthase MoaC -
  LG939_RS17965 (LG939_17970) - 3829093..3830826 (-) 1734 WP_028852633.1 PglL family O-oligosaccharyltransferase -
  LG939_RS17970 (LG939_17975) pilA 3830903..3831412 (-) 510 WP_020831292.1 pilin Machinery gene
  LG939_RS17975 (LG939_17980) pilA 3831678..3832199 (-) 522 WP_020831290.1 pilin Machinery gene
  LG939_RS17980 (LG939_17985) - 3832393..3833091 (-) 699 WP_020831289.1 TerC family protein -
  LG939_RS17985 (LG939_17990) sucD 3833241..3834122 (-) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  LG939_RS17990 (LG939_17995) sucC 3834207..3835373 (-) 1167 WP_011000513.1 ADP-forming succinate--CoA ligase subunit beta -
  LG939_RS17995 (LG939_18000) - 3835440..3836066 (-) 627 WP_011000512.1 DUF2889 domain-containing protein -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 17122.83 Da        Isoelectric Point: 9.9491

>NTDB_id=535596 LG939_RS17970 WP_020831292.1 3830903..3831412(-) (pilA) [Ralstonia pseudosolanacearum strain RS639]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAANAQTDLSVGSSVFT
PTKNVAGLAIDGTTTGVPGQITITYTQAAGGGTLALVPTAAGAKLPTSSAPSGPIMWTCYAAGKAQAASSVAPNATMSLA
AKYAPAECR

Nucleotide


Download         Length: 510 bp        

>NTDB_id=535596 LG939_RS17970 WP_020831292.1 3830903..3831412(-) (pilA) [Ralstonia pseudosolanacearum strain RS639]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACGGTTCGTGCACGCGTGACGGAAGGTCTGTCGCTGG
CTGCACAAGCCAAGGCACTGGTGTCGGAAAACGCTGCCAATGCACAGACTGACCTGTCGGTGGGTTCGTCGGTGTTCACG
CCGACCAAGAACGTTGCAGGCCTTGCAATTGACGGTACTACGACTGGCGTTCCGGGCCAGATCACCATCACCTATACGCA
GGCTGCCGGCGGCGGCACGCTGGCTCTGGTCCCGACCGCGGCAGGCGCTAAGCTGCCGACGAGTTCGGCTCCGTCGGGTC
CGATCATGTGGACCTGCTACGCTGCGGGTAAGGCACAGGCTGCCAGCAGCGTTGCTCCGAACGCTACGATGTCGCTGGCA
GCGAAGTATGCTCCGGCAGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(43-169)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

91.124

100

0.911
  pilA2 Legionella pneumophila str. Paris

51.235

95.858

0.491
  pilA2 Legionella pneumophila strain ERS1305867

50.617

95.858

0.485
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.462

100

0.444
  comP Acinetobacter baylyi ADP1

43.023

100

0.438
  pilA/pilA1 Eikenella corrodens VA1

38.506

100

0.396