Detailed information NTG0535536

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: I6J38_RS06515
• Genome accession: NZ_CP069558
• Coordinates: 1317992..1318429 (-)
• Length: 145 a.a.
• NCBI ID: WP_003035329.1
• Uniprot ID: F9P803
• Organism: Streptococcus constellatus strain FDAARGOS_1208
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1312992..1323429

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6J38_RS06495 (I6J38_06495)	glmS	1313196..1315010 (-)	1815	WP_020998064.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
I6J38_RS06500 (I6J38_06500)	-	1315401..1316600 (-)	1200	WP_006267970.1	acetate kinase	-
I6J38_RS06505 (I6J38_06505)	comYH	1316647..1317600 (-)	954	WP_020998065.1	class I SAM-dependent methyltransferase	Machinery gene
I6J38_RS06510 (I6J38_06510)	comGG	1317685..1318011 (-)	327	WP_020998066.1	competence type IV pilus minor pilin ComGG	-
I6J38_RS06515 (I6J38_06515)	comGF/cglF	1317992..1318429 (-)	438	WP_003035329.1	competence type IV pilus minor pilin ComGF	Machinery gene
I6J38_RS06520 (I6J38_06520)	comGE/cglE	1318413..1318706 (-)	294	WP_003035318.1	competence type IV pilus minor pilin ComGE	Machinery gene
I6J38_RS06525 (I6J38_06525)	comYD	1318678..1319106 (-)	429	WP_020999411.1	competence type IV pilus minor pilin ComGD	Machinery gene
I6J38_RS06530 (I6J38_06530)	comYC	1319066..1319383 (-)	318	WP_020998068.1	competence type IV pilus major pilin ComGC	Machinery gene
I6J38_RS06535 (I6J38_06535)	comYB	1319380..1320402 (-)	1023	WP_037592679.1	competence type IV pilus assembly protein ComGB	Machinery gene
I6J38_RS06540 (I6J38_06540)	comYA	1320344..1321285 (-)	942	WP_006268058.1	competence type IV pilus ATPase ComGA	Machinery gene
I6J38_RS06545 (I6J38_06545)	-	1321355..1321720 (-)	366	WP_022525692.1	DUF1033 family protein	-
I6J38_RS06550 (I6J38_06550)	glnA	1321908..1323254 (-)	1347	WP_020998070.1	type I glutamate--ammonia ligase	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16865.57 Da
• Isoelectric Point: 10.6422

>NTDB_id=535536 I6J38_RS06515 WP_003035329.1 1317992..1318429(-) (comGF/cglF) [Streptococcus constellatus strain FDAARGOS_1208]
MFKANKVKAFTLLEALVALFVISGSVLLFQSMTRLLASEVRARQHSEQREWLLFAHQLEAELIRSSFEKVENNRLYMKQD
GKTIAFGKSSGQDFRKTNANGKGYQPMIYNVKKAEISQKNELIHIRMTFKRGLEREFVYRVETKS

Nucleotide

• Length: 438 bp

>NTDB_id=535536 I6J38_RS06515 WP_003035329.1 1317992..1318429(-) (comGF/cglF) [Streptococcus constellatus strain FDAARGOS_1208]
GTGTTCAAGGCAAATAAAGTGAAAGCTTTTACACTTTTAGAGGCTCTGGTTGCCCTCTTTGTAATCAGCGGGAGTGTGCT
ATTATTTCAATCGATGACGCGTCTTCTGGCTTCGGAGGTCCGAGCTCGACAACACAGTGAACAACGAGAATGGCTTTTGT
TTGCCCATCAATTAGAAGCGGAATTGATTCGTTCTTCATTTGAAAAAGTGGAGAATAATCGTTTATACATGAAGCAGGAC
GGTAAGACGATTGCATTTGGAAAGTCAAGTGGACAAGATTTCAGAAAAACCAATGCAAATGGGAAAGGCTATCAGCCTAT
GATTTATAATGTGAAAAAAGCAGAAATCTCACAGAAAAATGAGCTGATTCATATCAGAATGACATTTAAGCGAGGATTGG
AAAGGGAGTTCGTCTATCGTGTGGAAACAAAGAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: F9P803

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae R6	66.429	96.552	0.641
comGF/cglF	Streptococcus pneumoniae TIGR4	66.429	96.552	0.641
comGF/cglF	Streptococcus pneumoniae Rx1	66.429	96.552	0.641
comGF/cglF	Streptococcus pneumoniae D39	66.429	96.552	0.641
comGF/cglF	Streptococcus mitis NCTC 12261	65.714	96.552	0.634
comGF/cglF	Streptococcus mitis SK321	65.714	96.552	0.634
comYF	Streptococcus mutans UA159	43.972	97.241	0.428
comYF	Streptococcus mutans UA140	43.972	97.241	0.428
comGF	Lactococcus lactis subsp. cremoris KW2	42.963	93.103	0.4