Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6J32_RS09725 Genome accession   NZ_CP069437
Coordinates   2007944..2009650 (+) Length   568 a.a.
NCBI ID   WP_204155407.1    Uniprot ID   -
Organism   Moraxella osloensis strain FDAARGOS_1202     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2002944..2014650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J32_RS09695 (I6J32_09705) nusA 2003627..2005108 (-) 1482 WP_062332403.1 transcription termination factor NusA -
  I6J32_RS09700 (I6J32_09710) rimP 2005226..2005735 (-) 510 WP_100270612.1 ribosome maturation factor RimP -
  I6J32_RS09715 (I6J32_09725) secG 2006299..2006619 (-) 321 WP_036594931.1 preprotein translocase subunit SecG -
  I6J32_RS09720 (I6J32_09730) tpiA 2006711..2007520 (-) 810 WP_204155406.1 triose-phosphate isomerase -
  I6J32_RS09725 (I6J32_09735) pilB 2007944..2009650 (+) 1707 WP_204155407.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6J32_RS09730 (I6J32_09740) pilC 2009707..2010930 (+) 1224 WP_159375395.1 type II secretion system F family protein Machinery gene
  I6J32_RS09735 (I6J32_09745) pilD 2010936..2011811 (+) 876 WP_204155408.1 prepilin peptidase Machinery gene
  I6J32_RS09740 (I6J32_09750) coaE 2011808..2012464 (+) 657 WP_228288305.1 dephospho-CoA kinase -
  I6J32_RS09745 (I6J32_09755) - 2012522..2013928 (-) 1407 WP_204155409.1 nicotinate phosphoribosyltransferase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62725.15 Da        Isoelectric Point: 5.2388

>NTDB_id=534666 I6J32_RS09725 WP_204155407.1 2007944..2009650(+) (pilB) [Moraxella osloensis strain FDAARGOS_1202]
MAASLPSFGGIASRLVHEGLVSEAAMQKVLAESQQQKINLIDYVVDNKLAQADAVAWVISREFGDPLIDLDAINVNYIPK
EAVDEKIIREFNILPIFQRGSRLFVAMSDPTRVDALDALRFGTRFTVETVVAEHHKIKSLIEKIFTTTNLADFDDMEAVE
FDETRADEDNSATSHLDVNDEAPVVKFVNGMLIKAITMGASDLHFEPYEKSYRVRFRIDGVLQKMANPPTQLAGKIAARL
KVMSQMDISERRVPQDGRIKLKISKDKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKQLFMDALAKPQG
MLLITGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLEGINQVNVNNKVGLTFASALKSFLRQDPDIVMVGEIRDL
ETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKSCKKPTDVPKNSLLEMGFTE
EDLADTSNVIYEPVGCSECRDGYKGRVGIYEVMKVTPEIARIIMEDGNAIEIKDAALKQGFRDLRRSGILKVLKGTTSIQ
EMYRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=534666 I6J32_RS09725 WP_204155407.1 2007944..2009650(+) (pilB) [Moraxella osloensis strain FDAARGOS_1202]
ATGGCGGCATCCCTTCCTAGTTTTGGTGGCATTGCATCGCGATTAGTGCATGAAGGACTAGTTAGCGAAGCGGCAATGCA
AAAAGTATTGGCAGAATCGCAGCAGCAAAAAATAAATTTAATCGACTATGTGGTTGACAATAAGCTTGCGCAAGCTGACG
CAGTAGCGTGGGTAATTTCGCGAGAATTTGGCGACCCACTCATTGATTTGGATGCCATCAATGTCAACTATATTCCAAAA
GAAGCGGTTGATGAAAAAATTATTCGCGAATTTAATATTCTGCCGATTTTCCAACGAGGTAGCCGCTTATTTGTTGCCAT
GAGCGACCCGACGCGGGTAGATGCTTTAGATGCACTGCGGTTCGGTACAAGGTTTACCGTGGAAACAGTGGTTGCCGAAC
ACCACAAAATCAAATCACTCATCGAAAAAATCTTTACTACCACCAATTTAGCTGATTTTGATGATATGGAAGCGGTGGAG
TTTGATGAAACCCGCGCCGATGAAGACAATAGTGCCACCAGTCATTTAGATGTCAATGATGAAGCGCCTGTCGTCAAGTT
CGTTAATGGGATGCTCATCAAAGCCATTACCATGGGCGCTTCAGACTTACACTTTGAACCCTATGAAAAATCTTATCGCG
TGCGTTTTCGTATTGATGGGGTGTTACAAAAAATGGCAAATCCACCGACGCAGCTAGCGGGTAAAATTGCTGCCCGCCTG
AAAGTCATGTCACAAATGGACATCTCTGAACGCCGCGTGCCGCAAGATGGTCGTATCAAATTAAAAATTTCCAAGGATAA
GGCGATTGATTTTCGTGTCAACTCATTGCCAACCTTATTTGGTGAAAAAATCGTACTGCGTATTTTAGACCCATCATCAG
CGATGCTAGGCATTGATGCACTCGGCTATGAGCCGGACCAAAAGCAACTATTTATGGATGCGCTGGCAAAACCGCAAGGG
ATGTTATTGATTACCGGCCCTACTGGTTCGGGTAAAACCGTCTCCTTATACACCGGTCTTAATATCTTAAACACAGTTGA
GACCAATATTTCTACCGCAGAAGATCCCGTTGAAATTAACCTTGAAGGCATCAATCAGGTCAACGTGAATAATAAAGTTG
GCTTGACCTTTGCCAGTGCGCTCAAATCATTCTTGCGTCAAGACCCTGATATTGTGATGGTCGGTGAGATTCGTGACTTG
GAAACGGCGGAAATTGCTGTCAAAGCTGCCCAAACAGGGCATATGGTACTGTCAACCTTGCATACCAACTCAGCGCCAGA
AACCTTAACGCGTCTGCGTAATATGGGTGTGGCATCCTTTAACATTGCCACCTCAGTCAATTTGGTAATCGCACAGCGCT
TAGCCCGTCGCCTGTGTAAATCCTGCAAAAAACCGACAGATGTACCAAAAAATAGCTTGCTTGAAATGGGTTTTACGGAA
GAAGATTTGGCAGATACCAGCAATGTCATTTATGAGCCCGTTGGCTGCTCTGAATGCCGCGATGGCTATAAAGGACGTGT
TGGTATTTATGAAGTGATGAAAGTAACACCAGAGATTGCCCGAATTATTATGGAAGACGGCAACGCTATTGAGATTAAAG
ACGCTGCCTTAAAGCAAGGGTTTAGGGATTTAAGGCGCTCTGGCATTTTAAAGGTTCTAAAAGGCACGACCAGCATCCAA
GAGATGTATCGTGTTACTAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.845

99.648

0.676
  pilB Acinetobacter baylyi ADP1

65.426

99.296

0.65
  pilB Legionella pneumophila strain ERS1305867

51.767

99.648

0.516
  pilF Neisseria gonorrhoeae MS11

49.374

98.415

0.486
  pilB Vibrio cholerae strain A1552

48.479

98.415

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

47.425

99.12

0.47
  pilB Vibrio campbellii strain DS40M4

46.607

98.592

0.46
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.734

91.197

0.371
  pilF Thermus thermophilus HB27

36.824

100

0.371