Detailed information NTG0534241

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: JQ490_RS11035
• Genome accession: NZ_CP069378
• Coordinates: 2234395..2235468 (+)
• Length: 357 a.a.
• NCBI ID: WP_010906319.1
• Uniprot ID: A0A552YT77
• Organism: Lactococcus lactis subsp. lactis bv. diacetylactis strain Ge001
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2229395..2240468

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JQ490_RS11030 (JQ490_11030)	comGA	2233563..2234501 (+)	939	WP_010906320.1	competence type IV pilus ATPase ComGA	Machinery gene
JQ490_RS11035 (JQ490_11035)	comGB	2234395..2235468 (+)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
JQ490_RS11040 (JQ490_11040)	comGC	2235596..2235865 (+)	270	WP_023349160.1	competence type IV pilus major pilin ComGC	Machinery gene
JQ490_RS11045 (JQ490_11045)	comGD	2235840..2236256 (+)	417	WP_021216554.1	competence type IV pilus minor pilin ComGD	Machinery gene
JQ490_RS11050 (JQ490_11050)	comGE	2236228..2236524 (+)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
JQ490_RS11055 (JQ490_11055)	comGF	2236487..2236933 (+)	447	WP_031296844.1	competence type IV pilus minor pilin ComGF	Machinery gene
JQ490_RS11060 (JQ490_11060)	comGG	2236972..2237256 (+)	285	WP_010906314.1	competence type IV pilus minor pilin ComGG	Machinery gene
JQ490_RS11065 (JQ490_11065)	-	2237337..2237774 (+)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
JQ490_RS11070 (JQ490_11070)	-	2237771..2238613 (+)	843	WP_010906312.1	metal ABC transporter substrate-binding protein	-
JQ490_RS11075 (JQ490_11075)	-	2238790..2239527 (+)	738	WP_010906311.1	metal ABC transporter ATP-binding protein	-
JQ490_RS11080 (JQ490_11080)	-	2239520..2240329 (+)	810	WP_010906310.1	metal ABC transporter permease	-

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 40330.51 Da
• Isoelectric Point: 10.3000

>NTDB_id=534241 JQ490_RS11035 WP_010906319.1 2234395..2235468(+) (comGB) [Lactococcus lactis subsp. lactis bv. diacetylactis strain Ge001]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide

• Length: 1074 bp

>NTDB_id=534241 JQ490_RS11035 WP_010906319.1 2234395..2235468(+) (comGB) [Lactococcus lactis subsp. lactis bv. diacetylactis strain Ge001]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCGTTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCGGGAATATAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (227-348)

• Type II secretion system (T2SS), protein F (T2SSF) (41-159)

3D structure

• AlphaFold DB: A0A552YT77

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	83.578	95.518	0.798
comYB	Streptococcus gordonii str. Challis substr. CH1	53.959	95.518	0.515
comYB	Streptococcus mutans UA140	53.372	95.518	0.51
comYB	Streptococcus mutans UA159	53.079	95.518	0.507
comGB/cglB	Streptococcus mitis SK321	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae TIGR4	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae Rx1	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae D39	51.045	93.838	0.479
comGB/cglB	Streptococcus pneumoniae R6	51.045	93.838	0.479
comGB/cglB	Streptococcus mitis NCTC 12261	50.448	93.838	0.473