Detailed information NTG0532823

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: JOS67_RS03485
• Genome accession: NZ_CP069195
• Coordinates: 612002..613687 (-)
• Length: 561 a.a.
• NCBI ID: WP_203346928.1
• Uniprot ID: -
• Organism: Vibrio diabolicus strain SLV18
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	605426..623422	612002..613687	within	0

Gene organization within MGE regions

Location: 605426..623422

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JOS67_RS03440 (JOS67_03435)	rpsP	605426..605674 (+)	249	WP_005379962.1	30S ribosomal protein S16	-
JOS67_RS03445 (JOS67_03440)	rimM	605704..606252 (+)	549	WP_005379964.1	ribosome maturation factor RimM	-
JOS67_RS03450 (JOS67_03445)	trmD	606280..607023 (+)	744	WP_203346927.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
JOS67_RS03455 (JOS67_03450)	rplS	607065..607418 (+)	354	WP_005379971.1	50S ribosomal protein L19	-
JOS67_RS03460 (JOS67_03455)	yacG	608084..608278 (-)	195	WP_005396692.1	DNA gyrase inhibitor YacG	-
JOS67_RS03465 (JOS67_03460)	zapD	608438..609178 (-)	741	WP_005396691.1	cell division protein ZapD	-
JOS67_RS03470 (JOS67_03465)	coaE	609206..609820 (-)	615	WP_047008979.1	dephospho-CoA kinase	-
JOS67_RS03475 (JOS67_03470)	pilD	609821..610690 (-)	870	WP_012841418.1	prepilin peptidase	Machinery gene
JOS67_RS03480 (JOS67_03475)	pilC	610755..611978 (-)	1224	WP_074191781.1	type II secretion system F family protein	Machinery gene
JOS67_RS03485 (JOS67_03480)	pilB	612002..613687 (-)	1686	WP_203346928.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JOS67_RS03490 (JOS67_03485)	pilA	613699..614136 (-)	438	WP_203346929.1	pilin	Machinery gene
JOS67_RS03495 (JOS67_03490)	nadC	614399..615286 (-)	888	WP_047102656.1	carboxylating nicotinate-nucleotide diphosphorylase	-
JOS67_RS03500 (JOS67_03495)	ampD	615379..615930 (+)	552	WP_012841421.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
JOS67_RS03505 (JOS67_03500)	pdhR	616336..617103 (+)	768	WP_005396680.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
JOS67_RS03510 (JOS67_03505)	aceE	617152..619815 (+)	2664	WP_005396679.1	pyruvate dehydrogenase (acetyl-transferring), homodimeric type	-
JOS67_RS03515 (JOS67_03510)	aceF	619835..621727 (+)	1893	WP_203346930.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-
JOS67_RS03520 (JOS67_03515)	lpdA	621995..623422 (+)	1428	WP_005379993.1	dihydrolipoyl dehydrogenase	-

Sequence

Protein

• Length: 561 a.a.
• Molecular weight: 62181.22 Da
• Isoelectric Point: 6.0043

>NTDB_id=532823 JOS67_RS03485 WP_203346928.1 612002..613687(-) (pilB) [Vibrio diabolicus strain SLV18]
MLTNLSTVLRQKGLLTFSQEEALIEQVKASGISMPEALLSSGLFTSSELAEHLSSLFGLNQPKLSRYEYASLCQQLGLRE
LITRHNALPLHRTTSTLLLAVVDPTNQQAEDDFRFATGLQVELVLADFRQLSAAIRRLYGRSLSHEKSGLKEINQEELAS
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSVCKQPQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide

• Length: 1686 bp

>NTDB_id=532823 JOS67_RS03485 WP_203346928.1 612002..613687(-) (pilB) [Vibrio diabolicus strain SLV18]
GTGCTAACTAACCTCTCCACGGTTCTTCGCCAAAAAGGGTTACTGACTTTTTCCCAAGAAGAAGCGCTAATAGAGCAAGT
TAAAGCCTCTGGCATCTCGATGCCAGAGGCTTTACTCAGTTCTGGATTATTTACATCAAGCGAACTCGCTGAGCACTTAA
GTTCTCTCTTTGGTTTAAATCAGCCCAAGTTATCTCGGTACGAGTACGCTTCGCTATGTCAGCAACTTGGCCTTAGGGAA
TTAATCACACGTCATAACGCACTCCCACTCCACCGTACGACTTCAACGTTATTACTCGCCGTTGTCGACCCCACGAACCA
ACAAGCAGAAGATGATTTCCGCTTTGCCACTGGGTTACAAGTTGAACTGGTCTTGGCGGATTTTCGTCAACTCAGCGCGG
CAATTCGACGCTTATATGGTCGTTCATTGAGTCATGAGAAGTCCGGATTAAAAGAGATCAACCAAGAAGAGTTAGCCAGT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATAAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGCGTTCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGTCGCTTATCTGCTCGTATCAAAATC
CTCTCTAAACTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTACCTACTCTATTCGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCTTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACCGGGAGCGGAAAAACCGTTTCTCTGTACACTGGGCTGAGTATTCTCAATAAACCAGAAATCAA
CATTTCCACAGCAGAAGATCCGGTAGAAATTAACTTATCGGGCATTAACCAAGTACAAGTACAGCCCAAGATCGGCTTTG
GCTTCGCAGAGGCGTTACGCTCTTTTCTTCGTCAAGACCCCGATGTAGTAATGGTCGGTGAGATTCGTGACTTAGACACT
GCGGAAATCGCCATAAAAGCATCGCAAACTGGTCACTTAGTACTTTCAACCTTGCATACCAACTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCCTCTCTTAGTTTGATTATAGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGGTCTGCAAGCAACCACAAGAGCTTACCGTTCAGTTGCAACATCTCGGTATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATAAAAGGCGCATCAGTGCATGAGTTAGAAAAACTCGCCATCGCAAACGGCA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGAATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (188-455)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (38-144)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Vibrio parahaemolyticus RIMD 2210633	95.722	100	0.957
pilB	Vibrio campbellii strain DS40M4	90.018	100	0.9
pilB	Vibrio cholerae strain A1552	74.555	100	0.747
pilB	Acinetobacter baumannii D1279779	51.521	93.761	0.483
pilB	Legionella pneumophila strain ERS1305867	49.353	96.435	0.476
pilB	Acinetobacter baylyi ADP1	50.484	92.157	0.465
pilF	Neisseria gonorrhoeae MS11	44.858	100	0.451
pilB/pilB1	Synechocystis sp. PCC 6803	37.625	100	0.401
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	36.684	100	0.371
pilF	Thermus thermophilus HB27	36.842	98.217	0.362