Detailed information NTG0529180

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: LLF72_RS06235
• Genome accession: NZ_CP068511
• Coordinates: 1230270..1231118 (+)
• Length: 282 a.a.
• NCBI ID: WP_015082464.1
• Uniprot ID: A0AAJ6N2R3
• Organism: Lactococcus cremoris strain F.2.7
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1225270..1236118

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLF72_RS06210 (LLF72_06060)	ilvN	1226003..1226479 (+)	477	WP_015082461.1	acetolactate synthase small subunit	-
LLF72_RS06215 (LLF72_06065)	ilvC	1226525..1227547 (+)	1023	WP_015082462.1	ketol-acid reductoisomerase	-
LLF72_RS06220 (LLF72_06070)	ilvA	1227606..1228856 (+)	1251	WP_014572605.1	threonine ammonia-lyase IlvA	-
LLF72_RS06225 (LLF72_06075)	budA	1228991..1229701 (+)	711	WP_011676302.1	acetolactate decarboxylase	-
LLF72_RS06230 (LLF72_06080)	-	1229741..1230121 (+)	381	WP_015082463.1	RidA family protein	-
LLF72_RS06235 (LLF72_06085)	dprA	1230270..1231118 (+)	849	WP_015082464.1	DNA-processing protein DprA	Machinery gene
LLF72_RS06240 (LLF72_06090)	topA	1231384..1233510 (+)	2127	WP_015082465.1	type I DNA topoisomerase	-
LLF72_RS06245 (LLF72_06095)	trmFO	1233582..1234928 (+)	1347	WP_014572600.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-
LLF72_RS06250 (LLF72_06100)	xerS	1234978..1236048 (-)	1071	WP_015082466.1	tyrosine recombinase XerS	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31854.03 Da
• Isoelectric Point: 9.8386

>NTDB_id=529180 LLF72_RS06235 WP_015082464.1 1230270..1231118(+) (dprA) [Lactococcus cremoris strain F.2.7]
MITNFDLFRWKKAGMTNLGVNKLLKFFLKYDIKISLRQMGQVAQVKSIPNFIEQYKNQDVKKLRIDYKKFSSFSILDDLY
PERLREIYNPPVLIFYQGNIDLLKNPKLAFVGSRLAGQSGIKAVQKIITELNQSFTIVSGLAKGIDTASHLSAIKTKTPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLVLSEYSLGEKPLKYHFPERNRIIAGLSRGVVVVEAKLRSGSLITCERALEEG
RDIFAIPGNIADGTSDGCNHLIQQGAKLVYQAQDILEEYLYN

Nucleotide

• Length: 849 bp

>NTDB_id=529180 LLF72_RS06235 WP_015082464.1 1230270..1231118(+) (dprA) [Lactococcus cremoris strain F.2.7]
ATGATTACAAATTTTGATTTATTTCGTTGGAAAAAAGCAGGAATGACCAATCTTGGTGTAAATAAGCTCTTAAAATTTTT
CCTCAAATATGATATAAAAATAAGCTTACGACAAATGGGACAAGTGGCTCAAGTCAAATCAATCCCTAACTTTATCGAAC
AATATAAAAATCAGGACGTAAAGAAACTCAGGATAGACTATAAAAAATTTTCCTCCTTTTCCATTCTTGATGACCTTTAT
CCCGAGCGTCTAAGAGAAATCTACAACCCACCCGTTTTAATTTTTTATCAGGGAAATATTGATTTATTAAAAAATCCTAA
ACTTGCCTTTGTTGGAAGCCGCCTAGCAGGTCAATCAGGGATAAAAGCTGTCCAAAAAATTATCACAGAATTGAATCAAT
CCTTTACAATAGTAAGCGGGCTTGCTAAAGGGATTGACACAGCCAGCCATCTGTCAGCAATTAAAACTAAAACACCAACA
ATTGCTGTTATTGGTACAGGATTGGATATTTTCTATCCACTAGAAAATCGTAAAATACAGGAATATCTAGCAAAAAATCA
ATTAGTTTTAAGTGAATATTCATTAGGCGAAAAACCACTTAAATACCACTTTCCAGAGCGCAATCGAATTATTGCTGGTC
TTTCACGGGGAGTTGTTGTCGTGGAAGCAAAATTAAGAAGTGGCAGTTTAATCACCTGTGAAAGGGCACTGGAAGAAGGA
AGAGATATCTTTGCCATCCCTGGAAATATTGCAGATGGAACATCTGATGGCTGTAATCATCTTATTCAACAAGGAGCAAA
ACTTGTCTATCAAGCTCAAGATATTTTAGAAGAATATTTGTACAATTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (69-270)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (2-63)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Lactococcus lactis subsp. cremoris KW2	97.872	100	0.979
dprA	Streptococcus mutans UA159	59.353	98.582	0.585
dprA/cilB/dalA	Streptococcus mitis SK321	56.071	99.291	0.557
dprA/cilB/dalA	Streptococcus pneumoniae R6	55.714	99.291	0.553
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	55.714	99.291	0.553
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	55.714	99.291	0.553
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	55.714	99.291	0.553
dprA/cilB/dalA	Streptococcus pneumoniae D39	55.714	99.291	0.553
dprA	Vibrio campbellii strain DS40M4	35.644	100	0.383
dprA	Bacillus subtilis subsp. subtilis str. 168	39.273	97.518	0.383
dprA	Legionella pneumophila strain ERS1305867	39.13	97.872	0.383
dprA	Haemophilus influenzae Rd KW20	39.63	95.745	0.379