Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JK628_RS01785 Genome accession   NZ_CP068437
Coordinates   395003..396712 (-) Length   569 a.a.
NCBI ID   WP_202287577.1    Uniprot ID   -
Organism   Shewanella sp. KX20019     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 390003..401712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JK628_RS01755 (JK628_01755) mutT 390036..390431 (-) 396 WP_202287571.1 8-oxo-dGTP diphosphatase MutT -
  JK628_RS01760 (JK628_01760) yacG 390428..390667 (-) 240 WP_202287572.1 DNA gyrase inhibitor YacG -
  JK628_RS01765 (JK628_01765) zapD 390716..391450 (-) 735 WP_202287573.1 cell division protein ZapD -
  JK628_RS01770 (JK628_01770) coaE 391459..392064 (-) 606 WP_202287574.1 dephospho-CoA kinase -
  JK628_RS01775 (JK628_01775) - 392662..393573 (-) 912 WP_202287575.1 A24 family peptidase -
  JK628_RS01780 (JK628_01780) pilC 393651..394922 (-) 1272 WP_202287576.1 type II secretion system F family protein Machinery gene
  JK628_RS01785 (JK628_01785) pilB 395003..396712 (-) 1710 WP_202287577.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JK628_RS01790 (JK628_01790) - 397107..398465 (+) 1359 WP_202287578.1 O-antigen ligase family protein -
  JK628_RS23410 (JK628_01795) - 398909..399271 (-) 363 WP_272931654.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63055.61 Da        Isoelectric Point: 5.4815

>NTDB_id=529057 JK628_RS01785 WP_202287577.1 395003..396712(-) (pilB) [Shewanella sp. KX20019]
MPSTGLHLGLSTLFIRKGLLSEEQLADAIAQSRKHKQSLVRTLITIKLLSAREVAELCYEEYGTPLLDLDEFDIAAIPDE
FLNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAVLVEDDKLIQALEKVLEEDITALDLSAIDEEA
LAGIEVTDTDKRDEEQTGNGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILQEVSEPPVNLSGRISA
RLKVMSKLDIAERRVPQDGRIKMKMSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRLMYEEMLKKP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTVEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVAGYNIASSVNLIIAQRLSRRLCQACKTPEVIPNDELLKLGF
SQLQLEQGITSYKPVGCEQCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKSQGMRDLRDSGLLKVIQGVTSI
AEVNRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=529057 JK628_RS01785 WP_202287577.1 395003..396712(-) (pilB) [Shewanella sp. KX20019]
ATGCCATCAACAGGCCTACATTTGGGATTATCGACACTTTTTATTCGCAAAGGACTGCTGAGCGAAGAACAGTTGGCGGA
CGCAATAGCGCAGTCCCGTAAACACAAGCAATCTTTAGTGCGCACCTTAATTACGATCAAACTATTATCAGCTCGTGAAG
TAGCCGAGCTTTGCTACGAAGAGTATGGCACCCCGCTACTGGATCTCGATGAGTTTGACATTGCCGCCATCCCCGATGAG
TTCCTCAATAAGAAGCTGATAGAGAAACATAAATGTTTGCCGCTGTTTAAGCGCGGTAACCGCCTCTATATTGCCACTTC
CGACCCTACTAACATTGCAGCGCTGGAGGATTTTCAGTTCAGTGCAGGCTTGCACGCCGAAGCGGTTTTAGTTGAAGATG
ACAAGCTGATTCAAGCCCTTGAGAAGGTGCTCGAAGAGGATATTACCGCGTTAGACCTGAGCGCTATCGATGAGGAAGCG
CTTGCGGGCATCGAGGTGACCGATACCGATAAACGCGATGAAGAGCAGACAGGAAATGGTAAAGATGACGCACCAATCGT
TATCTATATCAATAAAATTCTCACCGATGCTATTCGTAAAGGTGCATCGGATCTGCATTTTGAGCCCTATGAGAAGCGTT
ACCGCATCCGTTTTCGTATCGACGGCATATTACAGGAGGTCTCTGAGCCGCCGGTGAATCTGTCGGGGCGCATCTCCGCT
CGATTGAAGGTGATGTCAAAGCTTGACATAGCGGAGAGAAGAGTCCCGCAAGATGGTCGCATCAAGATGAAGATGTCCCG
CACTAAGTCGATCGATTTTCGTGTTAGTACCCTACCCACCATTTGGGGTGAGAAAATTGTAATGCGTATCTTGGACTCCT
CTTCGGCGCAACTCGGCATTGAAAAGCTCGGTTATGAAGATGATCAAAGACTGATGTACGAAGAGATGCTCAAAAAACCC
CAAGGGATGATCCTAGTTACCGGACCTACGGGTTCAGGTAAGACCGTCTCCCTGTATACCGGACTAAATATTCTTAATAC
CGCCGAGCGCAATATCTCCACCGTCGAGGATCCGGTTGAGATAAACCTTGAAGGCGTTAACCAGGTACACATTAATTTAA
AGGCCGGACTGACCTTTGCCTCGGCACTACGCTCATTTTTACGTCAAGATCCCGATGTGGTGATGGTCGGTGAGATTCGT
GACTTAGAGACCGCTGAAATTGCCATTAAGGCGGCGCAAACAGGTCACCTGGTATTATCGACCCTGCATACTAACTCCGC
CGCAGAGACCCTAACCCGCCTTATCAACATGGGCGTCGCCGGCTATAATATTGCCAGCTCGGTCAACTTAATTATTGCCC
AGCGTCTGTCGAGACGACTGTGCCAAGCCTGTAAAACTCCTGAAGTCATCCCCAATGATGAGTTGCTTAAACTAGGCTTT
AGCCAGCTGCAGCTCGAGCAAGGGATCACCTCCTATAAGCCTGTCGGCTGTGAGCAATGCTCTGGCGGCTATAAGGGCCG
TGTCGGTATTTACGAGGTGATGAAGATGTCTGACGAGATTGCCCGCACCATCATGGAGGGCGGTAATTCACTGCAAATTC
TCAAGCAAGCTAAATCACAGGGGATGCGTGACTTGCGAGATTCTGGGCTGTTAAAGGTGATCCAAGGGGTCACCAGTATT
GCAGAGGTTAACCGCGTCACTAGTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.111

98.594

0.573
  pilB Acinetobacter baumannii D1279779

57.624

99.121

0.571
  pilB Legionella pneumophila strain ERS1305867

55.872

98.77

0.552
  pilB Vibrio cholerae strain A1552

52.527

97.364

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

50.883

99.473

0.506
  pilB Vibrio campbellii strain DS40M4

50.089

98.946

0.496
  pilF Neisseria gonorrhoeae MS11

47.703

99.473

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

36.86

100

0.392
  pilF Thermus thermophilus HB27

38.83

99.121

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.843

96.134

0.364