Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JHW28_RS02265 Genome accession   NZ_CP068390
Coordinates   505540..507612 (+) Length   690 a.a.
NCBI ID   WP_162094297.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain YS21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 500540..512612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW28_RS02245 (JHW28_02245) pilM 502770..503822 (+) 1053 WP_202339259.1 pilus assembly protein PilM Machinery gene
  JHW28_RS02250 (JHW28_02250) - 503822..504388 (+) 567 WP_038582728.1 PilN domain-containing protein -
  JHW28_RS02255 (JHW28_02255) - 504385..505002 (+) 618 WP_202339260.1 type 4a pilus biogenesis protein PilO -
  JHW28_RS02260 (JHW28_02260) - 504999..505526 (+) 528 WP_162094296.1 pilus assembly protein PilP -
  JHW28_RS02265 (JHW28_02265) pilQ 505540..507612 (+) 2073 WP_162094297.1 type IV pilus secretin PilQ Machinery gene
  JHW28_RS02270 (JHW28_02270) aroK 507617..508135 (+) 519 WP_009041824.1 shikimate kinase AroK -
  JHW28_RS02275 (JHW28_02275) aroB 508284..509384 (+) 1101 WP_016704447.1 3-dehydroquinate synthase -
  JHW28_RS02280 (JHW28_02280) - 509395..510984 (+) 1590 WP_202339261.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74658.74 Da        Isoelectric Point: 5.4337

>NTDB_id=528867 JHW28_RS02265 WP_162094297.1 505540..507612(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain YS21]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLTNKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAAQSAPGQPAVASGRAKAAASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=528867 JHW28_RS02265 WP_162094297.1 505540..507612(+) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain YS21]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGGTGCCCGAGCCCCGGG
GTTACACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGACGAACAAGAGCCGCGAT
CTGGGCAATGGCAATGCACGCAGCGTGACAGTAGTCGAATCGGGGCAGCGTACCCGGGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGTACTCGAGTTGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCTC
CGGGGCAGCCGGCTGTCGCATCCGGGCGGGCCAAGGCTGCGGCTTCGGCGGGGCGGTCGATTCGCAGTGTGGATTTTCAG
CGCGGCGAGCAGGGTGAGGGCAATGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAACGCGACGG
CAAGCTCGTCCTCACCTTTGCCAAGGCCCGCCTGCCAGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACTC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGTGGTACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTCACGGTAAGCGTCAGGCCCATGACTTCGGGCGATCTGCAGAAACGCAACGCCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGTAAGGTGGGTAATGTGTTGCTGGTGGCGCCAGCCGATGA
AATCGCCGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGACCTGGAACCGCTGCGGCGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAAATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGACGAGCGT
GGGTCGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGTGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGC
TCTTCCACCATAGGCACGCCAGGCAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACTGACAACGTGTTGCTGGACCTTGAACTGACAGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTCGTTACCTCCGACAAAGAGACGGCGAAGATTCTCAAAGGTACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCAACTTCGGTGTCGTTCAAGGAGGCGTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTAGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

AMIN domain (AMIN)

(40-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.909

100

0.735
  comQ Acinetobacter baylyi ADP1

39.918

100

0.422
  pilQ Acinetobacter baumannii D1279779

37.987

100

0.41
  pilQ Acinetobacter baumannii strain A118

37.987

100

0.41
  pilQ Legionella pneumophila strain Lp02

38.039

97.536

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.117

96.957

0.37