Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   JHW28_RS02245 Genome accession   NZ_CP068390
Coordinates   502770..503822 (+) Length   350 a.a.
NCBI ID   WP_202339259.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain YS21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 497770..508822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW28_RS02235 (JHW28_02235) - 498722..499990 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  JHW28_RS02240 (JHW28_02240) - 500118..502553 (-) 2436 WP_164485365.1 penicillin-binding protein 1A -
  JHW28_RS02245 (JHW28_02245) pilM 502770..503822 (+) 1053 WP_202339259.1 pilus assembly protein PilM Machinery gene
  JHW28_RS02250 (JHW28_02250) - 503822..504388 (+) 567 WP_038582728.1 PilN domain-containing protein -
  JHW28_RS02255 (JHW28_02255) - 504385..505002 (+) 618 WP_202339260.1 type 4a pilus biogenesis protein PilO -
  JHW28_RS02260 (JHW28_02260) - 504999..505526 (+) 528 WP_162094296.1 pilus assembly protein PilP -
  JHW28_RS02265 (JHW28_02265) pilQ 505540..507612 (+) 2073 WP_162094297.1 type IV pilus secretin PilQ Machinery gene
  JHW28_RS02270 (JHW28_02270) aroK 507617..508135 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37037.47 Da        Isoelectric Point: 5.0008

>NTDB_id=528866 JHW28_RS02245 WP_202339259.1 502770..503822(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain YS21]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVSGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=528866 JHW28_RS02245 WP_202339259.1 502770..503822(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain YS21]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCAAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAAAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTTGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAAATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCGGC
CTGGCAAAAAAACAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTTCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.114

100

0.554
  comM Acinetobacter nosocomialis M2

54.83

100

0.551
  comM Acinetobacter baylyi ADP1

52.557

100

0.529
  pilM Legionella pneumophila strain ERS1305867

45.198

100

0.457