Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K8B88_RS04960 Genome accession   NZ_CP082774
Coordinates   1034571..1035956 (-) Length   461 a.a.
NCBI ID   WP_001025180.1    Uniprot ID   A0A0H2V435
Organism   Escherichia coli O16:H6 strain CU37RT-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1029571..1040956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B88_RS04925 (K8B88_04900) mutT 1029904..1030302 (+) 399 WP_000736047.1 8-oxo-dGTP diphosphatase MutT -
  K8B88_RS04930 (K8B88_04905) yacG 1030506..1030703 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  K8B88_RS04935 (K8B88_04910) zapD 1030713..1031456 (-) 744 WP_001194731.1 cell division protein ZapD -
  K8B88_RS04940 (K8B88_04915) coaE 1031456..1032076 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  K8B88_RS04945 (K8B88_04920) - 1032101..1032145 (+) 45 WP_120795372.1 protein YacM -
  K8B88_RS04950 (K8B88_04925) guaC 1032301..1033344 (+) 1044 WP_001217338.1 GMP reductase -
  K8B88_RS04955 (K8B88_04930) hofC 1033379..1034581 (-) 1203 WP_021523587.1 protein transport protein HofC -
  K8B88_RS04960 (K8B88_04935) pilB 1034571..1035956 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  K8B88_RS04965 (K8B88_04940) pilA 1035966..1036406 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  K8B88_RS04970 (K8B88_04945) nadC 1036610..1037503 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K8B88_RS04975 (K8B88_04950) ampD 1037591..1038142 (+) 552 WP_000923733.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K8B88_RS04980 (K8B88_04955) ampE 1038139..1038993 (+) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  K8B88_RS04985 (K8B88_04960) aroP 1039036..1040406 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50602.20 Da        Isoelectric Point: 6.5396

>NTDB_id=528763 K8B88_RS04960 WP_001025180.1 1034571..1035956(-) (pilB) [Escherichia coli O16:H6 strain CU37RT-2]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=528763 K8B88_RS04960 WP_001025180.1 1034571..1035956(-) (pilB) [Escherichia coli O16:H6 strain CU37RT-2]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACACGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTAGCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAACCAGGCACTGGATGTTAACACGCTTGGAATGCAGCCGTCACAACTGGTGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGATTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATAAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCCCCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2V435

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Legionella pneumophila strain ERS1305867

49.479

83.297

0.412
  pilB Acinetobacter baylyi ADP1

39.53

100

0.401
  pilB Vibrio campbellii strain DS40M4

47.927

83.731

0.401
  pilB Vibrio cholerae strain A1552

46.154

84.599

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

45.641

84.599

0.386
  pilB Haemophilus influenzae 86-028NP

44.703

83.948

0.375
  pilB Haemophilus influenzae Rd KW20

43.928

83.948

0.369
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilB Acinetobacter baumannii D1279779

42.893

85.466

0.367