Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K8B88_RS04965 Genome accession   NZ_CP082774
Coordinates   1035966..1036406 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli O16:H6 strain CU37RT-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1030966..1041406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B88_RS04940 (K8B88_04915) coaE 1031456..1032076 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  K8B88_RS04945 (K8B88_04920) - 1032101..1032145 (+) 45 WP_120795372.1 protein YacM -
  K8B88_RS04950 (K8B88_04925) guaC 1032301..1033344 (+) 1044 WP_001217338.1 GMP reductase -
  K8B88_RS04955 (K8B88_04930) hofC 1033379..1034581 (-) 1203 WP_021523587.1 protein transport protein HofC -
  K8B88_RS04960 (K8B88_04935) pilB 1034571..1035956 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  K8B88_RS04965 (K8B88_04940) pilA 1035966..1036406 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  K8B88_RS04970 (K8B88_04945) nadC 1036610..1037503 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K8B88_RS04975 (K8B88_04950) ampD 1037591..1038142 (+) 552 WP_000923733.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K8B88_RS04980 (K8B88_04955) ampE 1038139..1038993 (+) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  K8B88_RS04985 (K8B88_04960) aroP 1039036..1040406 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=528764 K8B88_RS04965 WP_000360914.1 1035966..1036406(-) (pilA) [Escherichia coli O16:H6 strain CU37RT-2]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=528764 K8B88_RS04965 WP_000360914.1 1035966..1036406(-) (pilA) [Escherichia coli O16:H6 strain CU37RT-2]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363