Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABCA12_RS14185 Genome accession   NZ_CP068253
Coordinates   2968005..2969729 (+) Length   574 a.a.
NCBI ID   WP_005403575.1    Uniprot ID   -
Organism   Acinetobacter junii strain CAM121     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2963005..2974729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABCA12_RS14150 (ABCA12_2900) nusA 2963857..2965341 (-) 1485 WP_004916840.1 transcription termination factor NusA -
  ABCA12_RS14155 (ABCA12_2901) rimP 2965379..2965903 (-) 525 WP_004916844.1 ribosome maturation factor RimP -
  ABCA12_RS14175 (ABCA12_2902) secG 2966526..2966855 (-) 330 WP_004916847.1 preprotein translocase subunit SecG -
  ABCA12_RS14180 (ABCA12_2903) tpiA 2966868..2967659 (-) 792 WP_004958617.1 triose-phosphate isomerase -
  ABCA12_RS14185 (ABCA12_2904) pilB 2968005..2969729 (+) 1725 WP_005403575.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABCA12_RS14190 (ABCA12_2905) pilC 2969754..2970980 (+) 1227 WP_201897849.1 type II secretion system F family protein Machinery gene
  ABCA12_RS14195 (ABCA12_2906) pilD 2970980..2971840 (+) 861 WP_201897850.1 prepilin peptidase Machinery gene
  ABCA12_RS14200 (ABCA12_2907) coaE 2971842..2972441 (+) 600 WP_201897851.1 dephospho-CoA kinase -
  ABCA12_RS14205 (ABCA12_2908) - 2972438..2973349 (-) 912 WP_201897852.1 DMT family transporter -
  ABCA12_RS14210 (ABCA12_2909) rlmB 2973380..2974129 (-) 750 WP_004916859.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABCA12_RS14215 (ABCA12_2910) - 2974318..2974644 (-) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63692.86 Da        Isoelectric Point: 5.0709

>NTDB_id=528641 ABCA12_RS14185 WP_005403575.1 2968005..2969729(+) (pilB) [Acinetobacter junii strain CAM121]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
PREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIEQQFGDSGSFDFGENDD
FDLDNVDVATTDTNNTTEDEDTNNQEESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LEKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSECKQPIEIPKQSLL
EMGFTEQDLANPDFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQ
GITSLQEVNRVTSE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=528641 ABCA12_RS14185 WP_005403575.1 2968005..2969729(+) (pilB) [Acinetobacter junii strain CAM121]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATCTCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CCTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTCATACAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAATTGAATATCGAACCTGTGATTG
TTGAACACAATAAACTCGAAAAACTAATTGAACAGCAGTTTGGAGATAGTGGCAGTTTCGATTTTGGGGAAAATGACGAC
TTTGATTTAGATAATGTTGATGTTGCAACGACTGATACAAACAATACGACTGAGGATGAGGATACAAATAATCAAGAAGA
ATCTCCTATCGTAAAATATATCAACAAGTTATTGATTGATGCGATTCGCATGGGGGCCTCTGACTTACATTTTGAGCCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGTGTACTCAGACAAATTGCTAACCCACCATTACAACTCGCAACT
CGTTTAGCCTCGCGCTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAGCGTATGCCTCAAGATGGTCGTATCAAACT
AAAACTCTCTAAAACTAAAGCGATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAGAAACTGGTTCTTCGTA
TCTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCATTAGGCTATGAGGATGAACAAAAAGAACTCTTTATGAGTGCA
TTAGAAAAGCCTCAGGGTATGCTTCTGATTACAGGTCCAACAGGCTCAGGGAAAACTGTATCGTTGTATACAGGTTTAAA
TATTTTAAATACAGAAGATACCAATATTTCGACCGCAGAAGATCCCGTCGAAATTAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACAGGTTTAACTTTCTCAGCAGCATTAAAAGCTTTTTTACGTCAAGATCCTGATGTCATCATGGTC
GGTGAGATTCGTGATTTGGAAACAGCTGAAATTGCAATTAAAGCAGCTCAAACAGGTCACATGGTGATGTCGACCCTGCA
TACCAATAGCGCTCCTGAAACACTCACACGATTACGCAATATGGGGGTTCCCTCTTTCAATATTGCAACCTCGGTTAATC
TCGTGATTGCTCAACGTTTAGCCCGCCGCCTATGCTCGGAATGTAAACAACCTATCGAGATTCCTAAGCAGAGCCTTTTA
GAAATGGGCTTCACTGAACAAGATTTAGCTAACCCAGACTTTCAAATTTTTCAACCTGTCGGCTGCGATGCTTGTCGTGA
AGGATATAAAGGGCGTGTCGGTATTTATGAAGTAATGAAAGTGACACCCGAGATTTCCAAGATTATTATGGAAGATGGAA
ATGCACTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTCAATAATCTACGCCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.424

100

0.817
  pilB Acinetobacter baylyi ADP1

76.87

100

0.77
  pilB Legionella pneumophila strain ERS1305867

52.788

93.728

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

50

97.213

0.486
  pilF Neisseria gonorrhoeae MS11

50.274

95.296

0.479
  pilB Vibrio cholerae strain A1552

48.917

96.516

0.472
  pilB Vibrio campbellii strain DS40M4

51.451

90.07

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

34.641

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.764

93.031

0.361