Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JJJ22_RS06925 Genome accession   NZ_CP068232
Coordinates   1404117..1405823 (-) Length   568 a.a.
NCBI ID   WP_200164279.1    Uniprot ID   -
Organism   Aeromonas caviae isolate Aero19     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1399117..1410823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJJ22_RS06895 (JJJ22_06895) mutT 1399746..1400150 (+) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -
  JJJ22_RS06900 (JJJ22_06900) yacG 1400206..1400400 (-) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  JJJ22_RS06905 (JJJ22_06905) zapD 1400411..1401133 (-) 723 WP_010675825.1 cell division protein ZapD -
  JJJ22_RS06910 (JJJ22_06910) coaE 1401171..1401785 (-) 615 WP_109111851.1 dephospho-CoA kinase -
  JJJ22_RS06915 (JJJ22_06915) pilD 1401804..1402679 (-) 876 WP_200164281.1 prepilin peptidase Machinery gene
  JJJ22_RS06920 (JJJ22_06920) pilC 1402747..1403988 (-) 1242 WP_200164280.1 type II secretion system F family protein Machinery gene
  JJJ22_RS06925 (JJJ22_06925) pilB 1404117..1405823 (-) 1707 WP_200164279.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  JJJ22_RS06930 (JJJ22_06930) - 1405827..1406267 (-) 441 WP_200164278.1 pilin -
  JJJ22_RS06935 (JJJ22_06935) nadC 1406577..1407464 (-) 888 WP_200164277.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JJJ22_RS06940 (JJJ22_06940) - 1407468..1407938 (-) 471 WP_042077632.1 retropepsin-like aspartic protease family protein -
  JJJ22_RS06945 (JJJ22_06945) ampD 1408051..1408620 (+) 570 WP_180907147.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JJJ22_RS06950 (JJJ22_06950) pdhR 1408899..1409663 (+) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62621.98 Da        Isoelectric Point: 5.8204

>NTDB_id=528341 JJJ22_RS06925 WP_200164279.1 1404117..1405823(-) (pilB) [Aeromonas caviae isolate Aero19]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDENSRLDDSVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNLDQLGFDDRQKGQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKTPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=528341 JJJ22_RS06925 WP_200164279.1 1404117..1405823(-) (pilB) [Aeromonas caviae isolate Aero19]
ATGACCTCTAGCCTTAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCCTCACTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGTAAGCCCTTTGTCACCTTCCTGATTGAGAACGACATCCTCGACAGCAAGGCCCTCG
CCGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGGCACACACTCTATATCGCCATGTCGGA
TCCAACCAATGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGTCTGCACACCGAGGCCCTGCTGGTCGAGGAGAGCA
AGCTGACCGCCGCCATCGGCAAATTGATGGAGAGCGAGCAGGATACCCTCGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAGCTCGAGGTGTCCGACGAGAACTCCCGCCTCGACGACAGCGTCAATACGGCCGACGATGACGCCCCCATCGTCAA
GTACATCCACAAGATCATGATGGACGCCATCAAGCGCGGCGCATCGGATCTGCATTTCGAACCTTACGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAGATCGCCACCCCGCCGGTGAACCTGGCGAACCGTTTCGCGGCTCGC
TTAAAAGTCATGGCGCGGCTCGACATCGCCGAACGGCGTCTGCCCCAGGATGGCCGGATCAAGCTCAAGATTTCGCGCAA
CCGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAGAAGATCGTCATCCGGCTGCTGGACTCCTCTG
CCGCCCGTCTCAACCTCGATCAGCTCGGCTTCGACGACCGCCAGAAGGGGCAATACCTGCACGCCCTCTCCAAGCCCCAG
GGGATGATCCTGGTCACCGGCCCCACGGGGTCGGGCAAGACGGTATCGCTCTATACCGGTCTCAACATCCTCAACACCAG
CGAGGTCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTGAACCAGGTGCAGATCAACCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGTCAGGATCCCGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTGTCCACCCTGCACACCAATTCGGCGGC
CGAGACGCTCACCCGGATGATGAACATGGGCGTCCCCGCCTTCAACATCGCCTCCTCGGTCACCCTCATCATGGCCCAGC
GCCTCGCCCGCAAGCTGTGCGATCATTGCAAGACGCCCGAAGTGGTGCCGGAAGCCGAGCTGCTGGAGCTGGGCTTTACC
CAGCAGCAGCTGGCCGCCGGCTTGCGGCTGTTCAAGCCCGTGGGGTGCAAGGAGTGCTCGGGAGGCTACCGGGGGCGGGT
CGGGATCTATGAGATCATGCTGATGTCAGACAACATCGCCAAGCTCATCATGCAAGGAGCCAACTCGCTGCAAATAGCGG
CCATTGCCCAGAAGGAAGGGATGCGCACCCTGCGTACCTCGGGCCTTGAAAAAGCCCGCCTGGGGGTCACCAGCCTGGCC
GAAATAAACAGGATCACTACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.885

99.12

0.544
  pilB Acinetobacter baumannii D1279779

54.497

99.824

0.544
  pilB Legionella pneumophila strain ERS1305867

54.078

99.296

0.537
  pilB Vibrio cholerae strain A1552

53.24

100

0.535
  pilB Vibrio campbellii strain DS40M4

49.911

99.12

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.734

99.12

0.493
  pilF Neisseria gonorrhoeae MS11

46.032

99.824

0.46
  pilF Thermus thermophilus HB27

39.962

92.958

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

37.862

97.183

0.368