Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JKK46_RS20245 Genome accession   NZ_CP068229
Coordinates   4565371..4567077 (+) Length   568 a.a.
NCBI ID   WP_107352321.1    Uniprot ID   -
Organism   Shewanella algae strain 150735     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4560371..4572077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JKK46_RS20220 (JKK46_20225) pdhR 4560511..4561263 (-) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  JKK46_RS20225 (JKK46_20230) ampE 4561538..4562389 (-) 852 WP_208128661.1 beta-lactamase regulator AmpE -
  JKK46_RS20230 (JKK46_20235) ampD 4562443..4563021 (-) 579 WP_208128662.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JKK46_RS20235 (JKK46_20240) nadC 4563180..4564040 (+) 861 WP_096142088.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JKK46_RS20240 (JKK46_20245) - 4564909..4565349 (+) 441 WP_045283208.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JKK46_RS20245 (JKK46_20250) pilB 4565371..4567077 (+) 1707 WP_107352321.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JKK46_RS20250 (JKK46_20255) pilC 4567238..4568503 (+) 1266 WP_045283209.1 type II secretion system F family protein Machinery gene
  JKK46_RS20255 (JKK46_20260) pilD 4568572..4569486 (+) 915 WP_208128663.1 A24 family peptidase Machinery gene
  JKK46_RS20260 (JKK46_20265) coaE 4569487..4570098 (+) 612 WP_028779563.1 dephospho-CoA kinase -
  JKK46_RS20265 (JKK46_20270) zapD 4570095..4570835 (+) 741 WP_028779562.1 cell division protein ZapD -
  JKK46_RS20270 (JKK46_20275) yacG 4570880..4571092 (+) 213 WP_025012057.1 DNA gyrase inhibitor YacG -
  JKK46_RS20275 (JKK46_20280) mutT 4571092..4571490 (+) 399 WP_145839803.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62975.12 Da        Isoelectric Point: 5.2568

>NTDB_id=528302 JKK46_RS20245 WP_107352321.1 4565371..4567077(+) (pilB) [Shewanella algae strain 150735]
MPTSGLHLGLSTLFIRKGLLDESSLAEAIKQSQKSKQALVTTLVTSKLVSARTIAELCYEEYGTPLLDLNEFDLSSIPDE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLDLDGLDEES
LAGIEVTDADKRQQEENEGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMRLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPEHELLQLGFT
QQQIDAGFTPYKPCGCEFCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIATTAKQQGMRDLRLSGLLKVIQGVTSIA
EINRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=528302 JKK46_RS20245 WP_107352321.1 4565371..4567077(+) (pilB) [Shewanella algae strain 150735]
ATGCCTACCTCCGGCCTCCATTTAGGATTATCTACACTCTTTATCCGTAAGGGGCTACTCGATGAGAGTAGCCTGGCGGA
GGCCATAAAGCAGTCACAAAAGAGTAAACAAGCACTGGTCACCACTCTGGTGACCAGTAAGCTTGTATCCGCCCGTACCA
TCGCCGAACTCTGTTATGAAGAGTATGGCACCCCGCTGCTGGATCTCAATGAGTTTGATTTAAGCAGTATCCCGGATGAG
TTTCTCAATAAAAAGCTAATCGACAAGCACCGCTGCCTGCCGTTATTCAAGCGTGGCAACCGTCTCTATATCGCCACCTC
GGATCCTACCAATATCGCCGCCCTGGAAGATTTTCAGTTCAGCGCCGGCCTGCACGCCGAAGCGATACTGGTAGAGGAAG
ATAAGCTCAGTAAAGCGCTGGAAAAAGTACTCGAAGAAGATTTGAGTTCACTGGACCTGGATGGTCTCGACGAGGAATCC
CTCGCCGGTATCGAGGTCACAGATGCTGACAAGCGTCAGCAGGAAGAGAATGAAGGCAAGGATGACGCCCCAATAGTCAT
CTATATCAATAAGATTTTGACCGATGCCATCCGCAAGGGCGCATCGGATCTGCATTTCGAGCCCTACGAGAAGCGCTACC
GCATTCGCTTTCGTATCGATGGTATTCTCCACGAGGTATCTGAGCCCCCCATTTCTCTCTCAGGCCGTATATCGGCCCGC
TTGAAGGTAATGTCCAAACTGGATATCGCCGAGCGCCGAGTACCCCAGGATGGCCGCATCAAGATGAGGCTGTCGCGCAA
CAAGAGTATCGATTTCCGGGTCAGCACCCTGCCCACCCTCTGGGGTGAAAAAATCGTGATGCGGATCCTCGATTCCTCTT
CCGCGCAATTAGGAATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTATCTGGAAATGTTGGCAAGGCCCCAG
GGAATGATTCTGGTAACCGGCCCTACCGGCTCTGGTAAAACCGTGTCCCTCTATACTGGCCTCAATATCCTCAATACCGA
AGAACGCAATATCAGCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGGGTCAACCAGGTGCATATCAACCCCAAGG
CAGGGCTAACCTTCGCCTCGGCGCTGCGCTCCTTTTTGCGTCAGGATCCGGATGTGGTGATGGTGGGGGAAATTCGCGAC
CTAGAAACCGCCGAAATCGCCATCAAGGCGGCGCAAACCGGCCACTTGGTACTCTCAACCCTACATACCAACTCATCGGC
CGAAACTCTCACCCGCCTGCTCAACATGGGAGTCCCAGGTTATAACATTGCCAGTTCGGTCAACCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTCTGCCCCGAATGCCGCCAACCGGAAGATATCCCCGAGCATGAACTGCTGCAACTGGGCTTCACT
CAGCAGCAGATAGACGCAGGCTTTACCCCATATAAGCCCTGCGGCTGCGAATTCTGCTCCGGTGGCTACAAGGGCCGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGATGAAATCGCCAGAACCATTATGGAGGGCGGTAATTCGCTGCAGATTGCCA
CCACCGCCAAACAGCAAGGGATGCGCGATCTGCGCCTTTCCGGCCTTTTAAAGGTGATTCAAGGCGTCACCAGTATTGCC
GAGATAAATCGGGTCACCAGCTATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.18

98.768

0.585
  pilB Acinetobacter baumannii D1279779

57.923

100

0.579
  pilB Legionella pneumophila strain ERS1305867

56.661

99.12

0.562
  pilB Vibrio parahaemolyticus RIMD 2210633

53.263

99.824

0.532
  pilB Vibrio cholerae strain A1552

53.69

95.423

0.512
  pilB Vibrio campbellii strain DS40M4

51.51

99.12

0.511
  pilF Neisseria gonorrhoeae MS11

47.434

99.472

0.472
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.241

98.063

0.375
  pilF Thermus thermophilus HB27

37.766

99.296

0.375