Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JKK44_RS02210 Genome accession   NZ_CP068228
Coordinates   462878..464584 (-) Length   568 a.a.
NCBI ID   WP_208166119.1    Uniprot ID   -
Organism   Shewanella algae strain G1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 457878..469584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JKK44_RS02180 (JKK44_02180) mutT 458464..458862 (-) 399 WP_115389375.1 8-oxo-dGTP diphosphatase MutT -
  JKK44_RS02185 (JKK44_02185) yacG 458862..459074 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  JKK44_RS02190 (JKK44_02190) zapD 459119..459859 (-) 741 WP_208166116.1 cell division protein ZapD -
  JKK44_RS02195 (JKK44_02195) coaE 459856..460467 (-) 612 WP_208166117.1 dephospho-CoA kinase -
  JKK44_RS02200 (JKK44_02200) pilD 460468..461382 (-) 915 WP_144164451.1 prepilin peptidase Machinery gene
  JKK44_RS02205 (JKK44_02205) pilC 461452..462717 (-) 1266 WP_208166118.1 type II secretion system F family protein Machinery gene
  JKK44_RS02210 (JKK44_02210) pilB 462878..464584 (-) 1707 WP_208166119.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JKK44_RS02215 (JKK44_02215) - 464605..465015 (-) 411 WP_208166120.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JKK44_RS02220 (JKK44_02220) nadC 465872..466732 (-) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JKK44_RS02225 (JKK44_02225) ampD 466924..467469 (+) 546 WP_025012051.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JKK44_RS02230 (JKK44_02230) ampE 467523..468374 (+) 852 WP_208166121.1 beta-lactamase regulator AmpE -
  JKK44_RS02235 (JKK44_02235) pdhR 468650..469402 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63075.24 Da        Isoelectric Point: 5.3534

>NTDB_id=528262 JKK44_RS02210 WP_208166119.1 462878..464584(-) (pilB) [Shewanella algae strain G1]
MPTTGLHLGLSTLFVRKSLLTEKQLAEAVRQSQNSKQTLVTTLVTSQLLSARTIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLDLNGLDEES
LAGIEVTDTDKRQQEEGESKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPEHELLQLGFT
QQQIDAGFTPYKPCGCEFCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAATAKQQGMRDLRLSGLLKVIQGVTSIA
EVNRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=528262 JKK44_RS02210 WP_208166119.1 462878..464584(-) (pilB) [Shewanella algae strain G1]
GTGCCAACTACTGGGCTTCATTTGGGATTATCGACACTGTTTGTTCGTAAAAGTTTACTTACGGAAAAACAGTTGGCAGA
GGCAGTTAGACAATCCCAAAACAGTAAACAAACACTGGTCACCACTCTGGTGACCAGTCAACTTTTATCTGCCCGTACTA
TCGCCGAACTCTGCTACGAAGAGTATGGTACCCCGCTGCTGGATCTCAATGAGTTTGACTTAAGCAGTATTCCGGAAGAG
TTTCTCAACAAAAAACTGATCGAAAAACATAGGTGCCTGCCGTTATTTAAGCGTGGCAACCGCCTCTATATTGCCACTTC
AGACCCCACCAATATTGCCGCTCTGGAAGATTTTCAGTTCAGCGCAGGCTTGCATGCCGAAGCGATACTGGTAGAAGAGG
ATAAACTCAGCAAGGCGCTGGAGAAGGTGCTTGAAGAAGACTTGAGCTCACTGGATCTCAATGGCCTCGATGAGGAATCC
CTCGCAGGTATCGAGGTCACCGACACCGACAAGCGTCAACAGGAAGAGGGTGAAAGTAAAGACGATGCGCCTATCGTCAT
CTATATCAATAAGATTTTGACCGATGCTATCCGCAAGGGCGCATCGGATCTGCATTTCGAGCCTTACGAGAAACGCTACC
GCATCCGCTTTCGTATTGATGGCATCCTCCATGAGGTGTCCGAGCCACCAATTTCTCTCTCGGGCCGCATATCAGCCCGC
TTGAAGGTAATGTCCAAATTGGATATCGCCGAGCGCCGGGTACCTCAGGATGGCCGCATCAAGATGAAGCTTTCCCGTAA
CAAGAGTATCGATTTCCGGGTCAGTACCCTGCCCACCCTCTGGGGCGAAAAGATAGTGATGCGGATCCTCGACTCCTCTT
CCGCGCAATTAGGCATCGAAAAACTCGGCTATGAGGAAGACCAAAAGCAAGCCTACCTGGAGATGCTGGCAAGGCCCCAG
GGGATGATTCTGGTAACAGGCCCTACCGGCTCTGGTAAAACCGTATCCCTTTATACTGGCCTCAATATCCTCAATACCGA
AGAGCGTAATATCAGCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGGGTCAACCAGGTACATATCAACCCCAAGG
CCGGGCTAACCTTCGCTTCGGCGCTGCGCTCTTTTTTACGTCAGGATCCGGATGTGGTGATGGTGGGCGAAATTCGCGAC
CTGGAAACCGCTGAAATCGCCATCAAGGCGGCACAAACCGGCCACTTGGTACTCTCAACCCTGCATACCAACTCATCGGC
CGAAACCCTTACCCGCCTGCTTAATATGGGGGTACCAGGTTATAACATTGCCAGTTCGGTCAACCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTCTGCCCCGAATGCCGTCAACCGGAAGATATCCCCGAGCATGAACTGCTGCAACTGGGCTTCACT
CAGCAGCAGATAGACGCAGGCTTTACCCCATACAAGCCCTGCGGCTGCGAATTCTGCTCAGGTGGCTACAAGGGCCGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGATGAAATCGCCAGAACCATTATGGAGGGCGGTAACTCACTGCAGATTGCCG
CTACCGCCAAGCAGCAAGGGATGCGCGACCTGCGCCTTTCAGGCCTTTTAAAAGTGATTCAAGGCGTCACCAGTATCGCC
GAAGTGAACCGGGTCACCAGCTATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.858

99.12

0.593
  pilB Acinetobacter baylyi ADP1

58.897

98.944

0.583
  pilB Legionella pneumophila strain ERS1305867

56.36

99.648

0.562
  pilB Vibrio parahaemolyticus RIMD 2210633

53.263

99.824

0.532
  pilB Vibrio cholerae strain A1552

52.585

98.768

0.519
  pilB Vibrio campbellii strain DS40M4

51.865

99.12

0.514
  pilF Neisseria gonorrhoeae MS11

48.673

99.472

0.484
  pilF Thermus thermophilus HB27

37.943

99.296

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.259

95.07

0.373