Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6I49_RS09530 Genome accession   NZ_CP068206
Coordinates   1540581..1542293 (-) Length   570 a.a.
NCBI ID   WP_114837769.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain FDAARGOS_1092     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1535581..1547293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I49_RS09500 (I6I49_09490) - 1535659..1535982 (+) 324 WP_078388524.1 pyrimidine/purine nucleoside phosphorylase -
  I6I49_RS09505 (I6I49_09495) rlmB 1536125..1536874 (+) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I6I49_RS09510 (I6I49_09500) - 1536906..1537823 (+) 918 WP_201953373.1 DMT family transporter -
  I6I49_RS09515 (I6I49_09505) coaE 1537837..1538436 (-) 600 WP_201953376.1 dephospho-CoA kinase -
  I6I49_RS09520 (I6I49_09510) pilD 1538445..1539305 (-) 861 WP_201953379.1 A24 family peptidase Machinery gene
  I6I49_RS09525 (I6I49_09515) - 1539305..1540550 (-) 1246 Protein_1496 type II secretion system F family protein -
  I6I49_RS09530 (I6I49_09520) pilB 1540581..1542293 (-) 1713 WP_114837769.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6I49_RS09535 (I6I49_09525) tpiA 1542573..1543364 (+) 792 WP_114837770.1 triose-phosphate isomerase -
  I6I49_RS09540 (I6I49_09530) secG 1543378..1543707 (+) 330 WP_058868796.1 preprotein translocase subunit SecG -
  I6I49_RS09570 (I6I49_09560) rimP 1544661..1545185 (+) 525 WP_004692708.1 ribosome maturation factor RimP -
  I6I49_RS09575 (I6I49_09565) nusA 1545225..1546709 (+) 1485 WP_004692706.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63616.97 Da        Isoelectric Point: 4.8447

>NTDB_id=527980 I6I49_RS09530 WP_114837769.1 1540581..1542293(-) (pilB) [Acinetobacter johnsonii strain FDAARGOS_1092]
MSVSNGLPKFTGFIRRLVEDGRMPAENMQQAMLSAKKAQQDIVPYLIENYKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDRLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEYPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRCSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=527980 I6I49_RS09530 WP_114837769.1 1540581..1542293(-) (pilB) [Acinetobacter johnsonii strain FDAARGOS_1092]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGCCTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAAGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAATTATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGAGAGCCTGTTTTTGACTTAAGTGTCTATGATTCGGTACAAATT
CTACGAGATCTGGTCGAAGATAGACTTATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCCACCAGCAATCCAACCAACATGGATGCAATGGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAATATCCTCAGCTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCCAATGACGAAGAAGATGACTCGCCTCAAGGGGATGAAGCACCGATCGT
CAAATACATCAATAAATTACTGATTGATGCGATTCGTATGGGCGCATCAGACTTGCACTTTGAACCTTATGAAAAATCTT
ACCGTGTCCGTTATCGAGTAGATGGGGTTCTGCGTCATATTGCCAGTCCACCACTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGAATTAAATTAAAGCTGTCGAA
AAGCAAAGCCATCGACTTCCGTGTCAACTCACTTCCAACCTTATTTGGCGAAAAGCTGGTTCTACGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTATCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATTTCAACTGCGGAAGACCCTGTTGAGATTAACTTAGAAGGCATTAATCAGGTCAATGTGAACAATA
AAGTCGGCCTCACCTTCTCTGCTGCGCTGAAGTCCTTCTTACGTCAAGATCCTGATATTGTGATGGTTGGTGAGATTCGC
GACCTTGAAACAGCTGAAATCGCAATTAAAGCTGCACAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGTAATATGGGGGTTCCATCTTTTAACATTGCGACATCGGTCAATTTGGTGATTGCAC
AGCGTTTGGCACGCCGCTTATGCAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGTTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGATTTCAAAGTTTATCAACCGGTGGGGTGTGCTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTTGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.702

100

0.807
  pilB Acinetobacter baylyi ADP1

76.14

100

0.761
  pilB Legionella pneumophila strain ERS1305867

53.745

93.684

0.504
  pilF Neisseria gonorrhoeae MS11

49.466

98.596

0.488
  pilB Vibrio cholerae strain A1552

48.501

99.474

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB Vibrio campbellii strain DS40M4

50

90.877

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

34.839

100

0.379
  pilF Thermus thermophilus HB27

36.888

100

0.37