Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6I53_RS03395 Genome accession   NZ_CP068176
Coordinates   600706..602427 (+) Length   573 a.a.
NCBI ID   WP_004994254.1    Uniprot ID   A0A7T9Z7A9
Organism   Acinetobacter ursingii strain FDAARGOS_1096     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 595706..607427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I53_RS03360 (I6I53_03365) nusA 596486..597970 (-) 1485 WP_004994250.1 transcription termination factor NusA -
  I6I53_RS03365 (I6I53_03370) rimP 598006..598530 (-) 525 WP_004991666.1 ribosome maturation factor RimP -
  I6I53_RS03385 (I6I53_03390) secG 599216..599545 (-) 330 WP_004991669.1 preprotein translocase subunit SecG -
  I6I53_RS03390 (I6I53_03395) tpiA 599555..600352 (-) 798 WP_004994252.1 triose-phosphate isomerase -
  I6I53_RS03395 (I6I53_03400) pilB 600706..602427 (+) 1722 WP_004994254.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6I53_RS03400 (I6I53_03405) pilC 602458..603684 (+) 1227 WP_004994255.1 type II secretion system F family protein Machinery gene
  I6I53_RS16775 (I6I53_03410) pilD 603684..604544 (+) 861 WP_004994264.1 A24 family peptidase Machinery gene
  I6I53_RS03410 (I6I53_03415) coaE 604547..605140 (+) 594 WP_004994266.1 dephospho-CoA kinase -
  I6I53_RS03415 (I6I53_03420) - 605122..606045 (-) 924 WP_004994268.1 DMT family transporter -
  I6I53_RS03420 (I6I53_03425) rlmB 606086..606835 (-) 750 WP_004994270.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I6I53_RS03425 (I6I53_03430) - 606955..607281 (-) 327 WP_004991681.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 64602.42 Da        Isoelectric Point: 5.8424

>NTDB_id=527807 I6I53_RS03395 WP_004994254.1 600706..602427(+) (pilB) [Acinetobacter ursingii strain FDAARGOS_1096]
MADDELKSKYPTYLDRLVATGFLTKEQIHRMFYEINKNNKFIVQYLVEAFDIKPSIIAEAISIEYEEPLFDLNTFNLSLI
PHGIIDEKLITKHKILPLSVKDHTLYIAVVDPSLTQDLDTIRFNSKKKLELVIVEHDKLERALEEVYGTNAQLEFLNSLH
NEDNENTEDILSEFSFDINADHEKDDTIVVKYLNKMLYDAIKMRASDLHFEPYAKIYRVRYRIDGVLHAISFPPLVLAPR
LTVRLKIMAKMDISERRSPQDGRIKLPVSQFKSIDFRVSSLPTLYGEKLVLRILDASSATLGIDALGYEEDQKALFLEAL
NKPQGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGVNQVNVNPKVGLTFAAALKSFLRQDPDIIMVG
EIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNLATSVNLVIAQRLARRLCSKCKEPIQIPRNSLIE
MGFTEEDLSHPKLRIYQPVGCSECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIQIADASEKLGFNNLRRSGLKKVMQG
ITSLQEVNRVTSE

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=527807 I6I53_RS03395 WP_004994254.1 600706..602427(+) (pilB) [Acinetobacter ursingii strain FDAARGOS_1096]
ATGGCAGATGATGAGCTAAAGTCAAAATATCCAACTTACCTGGATCGCTTAGTTGCGACTGGATTTCTGACAAAAGAACA
GATCCATCGGATGTTTTATGAAATAAATAAAAATAACAAATTTATTGTTCAGTATCTTGTAGAGGCCTTTGATATAAAGC
CATCAATTATTGCTGAAGCTATTTCTATTGAATATGAAGAACCCCTATTTGATCTTAATACATTTAATCTCTCTTTGATT
CCTCATGGCATTATCGATGAAAAATTAATCACAAAGCATAAAATTTTACCTCTAAGCGTAAAAGACCACACCTTATATAT
TGCTGTTGTAGACCCCTCTCTTACTCAAGATTTAGATACAATTCGCTTTAATAGTAAGAAAAAACTAGAACTTGTGATCG
TTGAACATGACAAATTGGAACGCGCACTTGAAGAGGTTTATGGTACAAACGCCCAGCTTGAATTTCTGAATAGTTTACAT
AATGAAGATAATGAAAATACTGAAGACATCTTAAGTGAGTTTAGCTTTGATATAAATGCAGACCATGAGAAAGACGACAC
TATTGTTGTTAAATATTTAAATAAAATGCTTTATGATGCAATTAAAATGCGGGCATCCGACCTTCATTTTGAACCTTATG
CCAAAATCTATCGCGTTAGATATCGTATCGATGGTGTTTTACATGCAATTTCATTCCCACCATTAGTACTCGCACCGAGA
CTAACTGTTCGCCTAAAAATTATGGCGAAGATGGATATCTCAGAACGGCGCTCTCCACAAGATGGTCGTATAAAATTACC
AGTCTCACAATTTAAATCTATCGATTTTCGTGTCAGCTCACTGCCTACATTATATGGTGAAAAATTAGTGCTACGTATTC
TGGATGCTTCTAGCGCAACATTGGGTATTGATGCGCTTGGCTACGAGGAAGATCAAAAAGCATTATTTTTAGAAGCACTG
AACAAGCCACAAGGTATGTTGCTAATTACAGGACCAACGGGTTCTGGTAAAACAGTTTCGTTATACACAGGCCTCAATAT
TCTTAATACTGAGAGTTCAAATATTTCCACAGCAGAAGATCCTGTGGAAATTAACCTTGAAGGTGTTAATCAGGTGAATG
TTAACCCCAAGGTGGGCTTAACCTTTGCCGCTGCGCTAAAGTCTTTTTTACGTCAGGATCCTGACATTATCATGGTGGGT
GAGATTCGTGATCTGGAAACAGCAGAAATCGCCATCAAGGCTGCGCAAACAGGTCACATGGTCATGTCTACGCTGCATAC
TAACAGTGCGCCAGAAACATTAACACGTTTACGCAATATGGGCGTGCCTTCATTTAATCTTGCAACGTCCGTGAACTTAG
TAATTGCTCAACGATTGGCCCGACGTCTTTGTAGTAAATGTAAAGAGCCAATTCAGATCCCCAGAAATAGTTTGATCGAG
ATGGGGTTTACAGAGGAGGATCTATCTCACCCTAAACTCCGTATTTACCAACCTGTGGGATGTTCAGAATGCCGTGAAGG
CTACAAAGGTCGTGTCGGGATTTACGAAGTCATGAAAGTCACACCTGAAATTTCGAGAATTATTATGGAAGATGGAAATG
CAATACAAATTGCAGATGCATCGGAAAAACTGGGGTTTAATAATCTTCGTCGCTCAGGTCTTAAAAAGGTGATGCAAGGG
ATTACTTCTTTACAGGAAGTCAATCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.576

100

0.689
  pilB Acinetobacter baylyi ADP1

66.724

100

0.672
  pilB Legionella pneumophila strain ERS1305867

50.462

94.415

0.476
  pilF Neisseria gonorrhoeae MS11

48.036

97.731

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

48.556

96.684

0.469
  pilB Vibrio cholerae strain A1552

47.748

96.859

0.462
  pilB Vibrio campbellii strain DS40M4

47.491

97.382

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

33.71

100

0.365