Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JKJ11_RS14830 Genome accession   NZ_CP068024
Coordinates   3178723..3180414 (+) Length   563 a.a.
NCBI ID   WP_038222999.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43133     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3173723..3185414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JKJ11_RS14810 (JKJ11_14800) pdhR 3175240..3176007 (-) 768 WP_006074432.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  JKJ11_RS14815 (JKJ11_14805) ampD 3176392..3176976 (-) 585 WP_006074431.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JKJ11_RS14820 (JKJ11_14810) nadC 3177112..3178002 (+) 891 WP_006074430.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JKJ11_RS14825 (JKJ11_14815) - 3178244..3178723 (+) 480 WP_006074427.1 pilin -
  JKJ11_RS14830 (JKJ11_14820) pilB 3178723..3180414 (+) 1692 WP_038222999.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JKJ11_RS14835 (JKJ11_14825) pilC 3180425..3181651 (+) 1227 WP_006074424.1 type II secretion system F family protein Machinery gene
  JKJ11_RS14840 (JKJ11_14830) pilD 3181694..3182563 (+) 870 WP_124940291.1 A24 family peptidase Machinery gene
  JKJ11_RS14845 (JKJ11_14835) coaE 3182566..3183174 (+) 609 WP_252038393.1 dephospho-CoA kinase -
  JKJ11_RS14850 (JKJ11_14840) zapD 3183206..3183946 (+) 741 WP_006074420.1 cell division protein ZapD -
  JKJ11_RS14855 (JKJ11_14845) yacG 3183968..3184162 (+) 195 WP_006074419.1 DNA gyrase inhibitor YacG -
  JKJ11_RS14860 (JKJ11_14850) rplS 3184362..3184715 (-) 354 WP_006074418.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62486.69 Da        Isoelectric Point: 5.7830

>NTDB_id=526341 JKJ11_RS14830 WP_038222999.1 3178723..3180414(+) (pilB) [Vibrio sp. SCSIO 43133]
MPSTLPSILRQANAIDETQETALYDLSMLSGCSVPEGATELNLCQAQELVAVLADVFGLPIVSIKDNDYASLCQQLGLRD
VITQYQALPISLRDNVLTVAVSDPTPSQLESDFKFASGLQIELGLANHQDINAAIRKIYGEGQEQWSQQTKELTSHELAN
LVQVNESELEHCDELMLDDSPVSRFIHQILLEAVSKKASDIHFEPYEHQYRIRLRCDGILIEAHQPSIQLSRRLSARLKI
LAKLDIAERRLPQDGRIKLKLSPHSATDIRVSTLPTMWGEKVVLRILDLNSARLDIEQLGYNDTQQALYLQALHKPQGMV
LMTGPTGSGKTVSLYAGLKLLNTPERNISTAEDPIEIHLDGINQVQVNPKIGFGFSAALRTFLRQDPDVVMVGEIRDLDT
AEIAVKAAQTGHLVLSTLHTNSALETITRLQNMGIESFNIVSSLSLIIAQRLARRLCSLCKRPDHLRKDLLRSLDISSCQ
RIYKANDVGCSKCNRGYSGRIGIYEVLVFSNELSAAVLQKASIQDLEVIAVSQGMMTLKQAGLEKLTLGLTSYQELQRVL
YFE

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=526341 JKJ11_RS14830 WP_038222999.1 3178723..3180414(+) (pilB) [Vibrio sp. SCSIO 43133]
ATCCCGTCCACTCTCCCATCTATTTTAAGACAGGCTAACGCCATCGATGAAACGCAAGAAACTGCGTTATATGACCTGTC
CATGTTATCTGGTTGCTCCGTTCCAGAAGGAGCAACTGAGCTCAACCTTTGTCAGGCTCAAGAGCTTGTTGCTGTTCTAG
CCGACGTTTTTGGACTCCCAATAGTATCGATAAAGGACAACGACTATGCGAGTTTGTGCCAACAACTCGGTTTACGGGAC
GTCATAACTCAATACCAAGCCTTACCTATTTCACTGCGCGACAACGTTCTAACCGTTGCGGTCAGCGATCCCACCCCCTC
GCAGTTAGAAAGTGATTTTAAGTTTGCCTCAGGATTACAAATCGAACTGGGTTTGGCTAACCATCAAGACATAAACGCCG
CAATACGCAAAATTTATGGAGAGGGACAAGAGCAATGGAGCCAACAGACTAAGGAACTCACCTCCCACGAACTGGCAAAC
TTAGTACAAGTGAATGAGAGTGAACTTGAGCATTGCGATGAGCTAATGCTGGATGACTCGCCTGTCAGCCGTTTCATTCA
CCAAATCCTATTAGAAGCCGTCAGCAAGAAAGCGTCCGATATTCATTTTGAGCCATACGAACACCAGTACCGAATTCGAC
TTCGCTGTGATGGTATTCTTATTGAGGCACATCAGCCATCGATCCAATTAAGCCGCAGACTATCTGCTCGCCTGAAGATT
CTCGCTAAGCTAGATATTGCTGAGCGCCGTCTTCCTCAAGATGGTCGAATCAAACTCAAACTATCACCCCACTCCGCCAC
CGATATCCGTGTATCAACCCTGCCAACCATGTGGGGAGAAAAGGTAGTACTGAGAATTCTCGACCTAAATTCTGCCCGTT
TGGATATCGAGCAATTAGGGTATAACGACACTCAACAAGCTCTTTACTTGCAAGCGTTGCACAAGCCTCAAGGAATGGTG
TTAATGACAGGGCCTACTGGAAGTGGCAAAACCGTCTCTCTCTATGCGGGACTCAAACTTCTTAATACCCCAGAGCGAAA
TATCTCAACTGCCGAAGACCCGATTGAAATACATCTAGATGGGATAAACCAAGTTCAAGTTAACCCTAAGATAGGCTTTG
GTTTTTCTGCAGCCTTGCGCACTTTTTTACGCCAAGATCCGGATGTGGTTATGGTGGGAGAAATACGAGATCTCGACACA
GCAGAGATTGCGGTTAAAGCGGCTCAAACAGGTCATCTAGTTCTGTCAACTTTACACACTAATTCCGCGCTTGAGACCAT
TACACGGTTACAAAATATGGGAATAGAGAGCTTCAACATCGTGTCTTCACTTTCGCTAATTATCGCGCAGCGGCTCGCTC
GCCGTTTGTGCTCTTTATGTAAGCGCCCAGATCACCTCCGTAAAGATCTGCTCCGCAGTCTCGATATATCCTCCTGCCAG
AGGATTTACAAAGCCAATGATGTTGGTTGTAGCAAATGTAATAGAGGCTATTCGGGTCGTATCGGTATTTACGAAGTGCT
GGTTTTTTCCAACGAGTTAAGCGCCGCCGTTCTTCAGAAAGCCAGCATTCAAGATCTTGAAGTCATTGCTGTGTCACAAG
GAATGATGACACTTAAACAAGCGGGGCTTGAAAAACTCACTCTTGGACTGACAAGCTACCAAGAACTGCAGCGTGTCCTT
TATTTCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-143)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

63.54

100

0.638
  pilB Vibrio campbellii strain DS40M4

61.032

99.822

0.609
  pilB Vibrio parahaemolyticus RIMD 2210633

60.568

100

0.606
  pilB Legionella pneumophila strain ERS1305867

45.57

98.224

0.448
  pilB Acinetobacter baumannii D1279779

46.923

92.362

0.433
  pilB Acinetobacter baylyi ADP1

47.732

90.053

0.43
  pilF Neisseria gonorrhoeae MS11

44.402

92.007

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.31

91.652

0.369
  pilF Thermus thermophilus HB27

38.783

93.428

0.362