Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JJN09_RS21815 Genome accession   NZ_CP067412
Coordinates   4824492..4826192 (+) Length   566 a.a.
NCBI ID   WP_249483721.1    Uniprot ID   -
Organism   Pseudomonas sp. HS6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4819492..4831192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJN09_RS21795 (JJN09_21765) - 4819754..4822705 (+) 2952 WP_249483718.1 DUF748 domain-containing protein -
  JJN09_RS21800 (JJN09_21770) - 4822705..4823001 (+) 297 WP_249483719.1 DUF2845 domain-containing protein -
  JJN09_RS21805 (JJN09_21775) - 4823167..4823523 (-) 357 WP_096820895.1 BON domain-containing protein -
  JJN09_RS21810 (JJN09_21780) - 4823822..4824265 (-) 444 WP_249483720.1 pilin -
  JJN09_RS21815 (JJN09_21785) pilB 4824492..4826192 (+) 1701 WP_249483721.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JJN09_RS21820 (JJN09_21790) pilC 4826195..4827412 (+) 1218 WP_249483722.1 type II secretion system F family protein Machinery gene
  JJN09_RS21825 (JJN09_21795) pilD 4827414..4828286 (+) 873 WP_249483723.1 A24 family peptidase Machinery gene
  JJN09_RS21830 (JJN09_21800) coaE 4828283..4828906 (+) 624 WP_249483724.1 dephospho-CoA kinase -
  JJN09_RS21835 (JJN09_21805) yacG 4828903..4829103 (+) 201 WP_096820889.1 DNA gyrase inhibitor YacG -
  JJN09_RS21840 (JJN09_21810) - 4829112..4829327 (-) 216 WP_007959584.1 hypothetical protein -
  JJN09_RS21845 (JJN09_21815) - 4829393..4830082 (-) 690 WP_249483725.1 energy-coupling factor ABC transporter permease -
  JJN09_RS21850 (JJN09_21820) - 4830216..4830842 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62586.61 Da        Isoelectric Point: 5.6826

>NTDB_id=525942 JJN09_RS21815 WP_249483721.1 4824492..4826192(+) (pilB) [Pseudomonas sp. HS6]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLPLVSYLVQNKLVKSSQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLTTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDG
LDVESIDDSKQDAIGVLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLLKEGFPE
ERIGSFTIYEPAGCDHCNGGYKGRTGIYEVVKNTPELQRLIMAEGNSLEIDSQMRLDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=525942 JJN09_RS21815 WP_249483721.1 4824492..4826192(+) (pilB) [Pseudomonas sp. HS6]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTACAGGCCGAACTGCTCACGGAAAAAAGCGCCCAGCAAGC
CTGGCAGCAGGCCCAGCGCAATCGCCTGCCGCTGGTCAGCTATCTGGTACAGAACAAACTGGTGAAGAGTTCACAGGTCG
CCGAGATTGCCTCGGAGCATTTCGGCATGGCGTTCCTCGACCTCAATTGCCTCGACAAAGAGACCCAGCCCAAGGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCCCTGTGGCGGCGCGGCAACAAGCTGTTCGTGGGGATTTCCGA
CCCGAGCAATCATCAGGCAATCAACGACATTCAGTTCAGCACCGGGCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCACCGACGCCATCGAGAAGTTCTTCGACACCCACGCCTCCGGCCTGGAGGACATGGCCGACGTCGACCTCGACGGG
CTGGATGTCGAGTCCATCGATGACAGCAAGCAGGACGCCATCGGCGTGCTGGACGCCGACGATGCGCCGGTGGTGCGTTT
CGTCCACAAGATGCTGCTGGACGCGATCAAGAGCGGTTCCTCAGACCTGCACTTCGAACCCTACGAAAAAAACTACCGGG
TGCGGGTGCGTACCGACGGCATCCTGCGCGAAGTGGCCAAGCCGCCGATTCAACTGGCAGGACGCATCGCCGCACGCCTC
AAGGTCATGGCCAGCCTCGACATCTCGGAACGGCGCAAACCTCAGGACGGGCGACTGAAAATGCGCCTGTCGAAAACCAA
GTCGATCGATTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CGCAGATCGGCATCGACGCCCTCGGCTACGAGCCGGAGCAGAAAGAACTCTACCTGGCCGCCCTGAAACAGCCGCAAGGC
ATGATTCTGGTCACCGGCCCCACCGGTTCCGGCAAGACCGTGTCGCTGTACACCGGGCTCAACATCCTCAATACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCGGTAGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCTAAGCAGG
GGCTGGACTTCGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGTGAAATCCGTGACTTG
GAGACCGCCGAGATCGCCATCAAAGCAGCGCAGACCGGTCACCTGGTGCTCTCCACGCTGCACACCAACAGCGCCGCCGA
GACACTCACCCGCTTGCACAACATGGGCATTCCCGGCTTCAACATCGCCACCTCGGTCAGCCTGATCATCGCCCAGCGTC
TGGCGCGAAAACTCTGCAGCCACTGCAAGAAGCCCATCGAGATTCCTCGCGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATTGGCAGTTTCACGATCTATGAGCCCGCCGGATGCGATCACTGCAACGGCGGCTACAAGGGACGCACCGGGAT
TTATGAAGTGGTGAAAAACACCCCCGAGCTGCAACGGCTGATCATGGCCGAGGGCAACTCATTGGAAATCGACAGCCAGA
TGCGCCTGGACGGCTTCAACGACCTGCGCACCTCGGGACTGATCAAGGCGATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGAGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.053

99.823

0.58
  pilB Acinetobacter baumannii D1279779

57.624

99.647

0.574
  pilB Legionella pneumophila strain ERS1305867

55.81

100

0.56
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

49.735

100

0.498
  pilF Neisseria gonorrhoeae MS11

49.735

99.823

0.496
  pilB Vibrio campbellii strain DS40M4

49.298

100

0.496
  pilF Thermus thermophilus HB27

36.918

98.587

0.364