Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JJN09_RS16460 Genome accession   NZ_CP067412
Coordinates   3655452..3657530 (-) Length   692 a.a.
NCBI ID   WP_249482666.1    Uniprot ID   -
Organism   Pseudomonas sp. HS6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3650452..3662530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJN09_RS16445 (JJN09_16420) - 3652077..3653675 (-) 1599 WP_249482664.1 AAA family ATPase -
  JJN09_RS16450 (JJN09_16425) aroB 3653686..3654786 (-) 1101 WP_249482665.1 3-dehydroquinate synthase -
  JJN09_RS16455 (JJN09_16430) aroK 3654929..3655447 (-) 519 WP_025110261.1 shikimate kinase AroK -
  JJN09_RS16460 (JJN09_16435) pilQ 3655452..3657530 (-) 2079 WP_249482666.1 type IV pilus secretin PilQ Machinery gene
  JJN09_RS16465 (JJN09_16440) - 3657544..3658068 (-) 525 WP_249482667.1 pilus assembly protein PilP -
  JJN09_RS16470 (JJN09_16445) pilO 3658065..3658688 (-) 624 WP_249482668.1 type 4a pilus biogenesis protein PilO -
  JJN09_RS16475 (JJN09_16450) - 3658685..3659251 (-) 567 WP_249482669.1 PilN domain-containing protein -
  JJN09_RS16480 (JJN09_16455) pilM 3659251..3660315 (-) 1065 WP_249482670.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75157.34 Da        Isoelectric Point: 5.4082

>NTDB_id=525933 JJN09_RS16460 WP_249482666.1 3655452..3657530(-) (pilQ) [Pseudomonas sp. HS6]
MNRIFSTLGFSLWIALLSPMVQAASLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVTSQLTSKTRD
LGSGNARSATVVEANDRTRLIINLSQLAPYDARVEGNTLFVVVGQGSKVTAPRPVTKAPRATPVAAPARTFTPTAKAIRG
VDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPSGT
FDYSTYQTDNKLTVSIRPLTVDDLQKRNADRNTYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=525933 JJN09_RS16460 WP_249482666.1 3655452..3657530(-) (pilQ) [Pseudomonas sp. HS6]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTGTCGCCGATGGTTCAAGCGGCCAGTCTGAA
GACGCTGGATGTTGCGGCGCTGCCGGGCGATCGCGTCGAGTTGAAGCTGTCGTTCGATGGGCCGCCGCCACAGCCCAAGG
GCTACACCACCGATTCGCCGGCGCGAATTGCCCTTGATCTGCCGGGGGTGACCAGCCAGCTCACGAGCAAGACCCGTGAT
CTCGGTAGTGGTAATGCGCGCTCCGCCACAGTGGTCGAGGCCAACGACCGTACCCGGTTGATTATCAACCTGTCGCAACT
GGCGCCGTATGACGCCCGGGTCGAGGGGAATACGCTGTTCGTGGTGGTCGGGCAAGGTTCCAAAGTCACCGCGCCGCGAC
CTGTCACCAAGGCTCCGCGCGCAACGCCGGTGGCCGCCCCGGCCCGGACGTTTACGCCGACGGCCAAGGCCATTCGTGGC
GTGGACTTCCAGCGTGGCACGGCGGGCGAGGGCAATGTCGTCATCGACCTGTCTGACCCGACCATCGCGCCGGACATTCA
GGAGCATGACGGCAAGATCATCCTCAATTTTGCTCGTACCCAACTTCCAGAACCATTGCGGGTGCGCCTCGACGTCAAGG
ACTTCGCTACCCCGGTGCAGTTCGTCAATGCCGGCGTCAGCGGGGATCGCGCGACCATCACCGTCGAGCCCAGCGGCACC
TTCGACTACTCCACTTACCAGACCGACAACAAGCTGACCGTGAGCATCCGGCCGTTGACTGTCGATGACCTGCAAAAGCG
CAACGCCGACCGCAACACCTATAGCGGCGAAAAGCTTTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTGCTGCAAC
TGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTTCAGGGCGGAATCACCTTGCGCCTGCAAAACGTG
CCGTGGGATCAGGCACTGGATCTGGTGCTCAAGACCAAAGGGCTCGACAAACGCAAGGTCGGCAATGTGTTGCTGGTGGC
GCCGGCAGATGAAATCGCCGCCCGCGAGCGGCAGGAGCTGGAGTCGCAGAAACAGATCGCCGAGCTGGCGCCGTTACGTC
GCGAGCTGCTGCAAGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGCGCCGAGGCCAAA
GTCGACGAAAGGGGTTCGATCACCGTTGATGAACGCACCAATAACATCATTGCCTACCAGACTCAGGATCGCCTCGACGA
GCTGCGAAGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTAATGATCGAGGCGCGGATCGTCGAGGCCAACGTCG
ATTACGACAAAAGCCTGGGCGTGCGCTGGGGCGGATCGATCCAGAACAAGGGCAACTGGAATACCTCCGGGGTCAGCAAT
GGCTCGTCGACCACCATCGGTACGCCGGGCAGCACCAGCACCAACTCACCGTTCGTCGACATGGGCACGGTCAACAACAC
GTCCGGAATCGGTATCGCGTTCATCACCGACAACGTGTTGCTGGACCTTGAACTGACGGCGATGGAGAAGACAGGCAACG
GTGAAATCGTCTCGCAGCCCAAGGTCGTCACCTCCGACAAGGAGACCGCGAAGATCCTCAAGGGCACCGAGATTCCGTAT
CAGGAAGCCAGTTCCAGCGGCGCTACGTCAGTGTCGTTCAAGGAGGCCTCGCTTTCGCTGGAGGTCACGCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTGAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAATGCCAAGGTGCTGGTGAACGACGGCGAAACCATCGTGATTGGTGGGGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Secretin and TonB N terminus short domain (STN)

(302-348)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.888

100

0.743
  comQ Acinetobacter baylyi ADP1

40.837

100

0.409
  pilQ Acinetobacter baumannii D1279779

37.802

100

0.408
  pilQ Acinetobacter baumannii strain A118

37.802

100

0.408
  pilQ Legionella pneumophila strain Lp02

38.519

97.543

0.376
  pilQ Legionella pneumophila strain ERS1305867

38.484

97.254

0.374