Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K5O18_RS08475 Genome accession   NZ_CP081939
Coordinates   1675135..1676559 (+) Length   474 a.a.
NCBI ID   WP_046097579.1    Uniprot ID   A0A0F5F317
Organism   Avibacterium paragallinarum strain p4chr1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1670135..1681559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5O18_RS08445 (K5O18_08445) lgt 1670710..1671510 (-) 801 WP_017807046.1 prolipoprotein diacylglyceryl transferase -
  K5O18_RS08450 (K5O18_08450) - 1671520..1672311 (-) 792 WP_110479819.1 sulfite exporter TauE/SafE family protein -
  K5O18_RS08455 (K5O18_08455) rppH 1672314..1672907 (-) 594 WP_035687029.1 RNA pyrophosphohydrolase -
  K5O18_RS08460 (K5O18_08460) - 1673479..1673898 (-) 420 WP_133247373.1 hypothetical protein -
  K5O18_RS08465 (K5O18_08465) ampD 1673997..1674551 (-) 555 WP_110479836.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5O18_RS08470 (K5O18_08470) pilA 1674691..1675122 (+) 432 WP_035687024.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K5O18_RS08475 (K5O18_08475) pilB 1675135..1676559 (+) 1425 WP_046097579.1 GspE/PulE family protein Machinery gene
  K5O18_RS08480 (K5O18_08480) pyrH 1676809..1677525 (+) 717 WP_035687020.1 UMP kinase -
  K5O18_RS08485 (K5O18_08485) frr 1677550..1678107 (+) 558 WP_017807054.1 ribosome recycling factor -
  K5O18_RS08490 (K5O18_08490) ispC 1678289..1679485 (+) 1197 WP_110479821.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
  K5O18_RS08495 (K5O18_08495) uppS 1679639..1680367 (+) 729 WP_110479822.1 polyprenyl diphosphate synthase -
  K5O18_RS08500 (K5O18_08500) - 1680371..1681234 (+) 864 WP_110479823.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 53761.53 Da        Isoelectric Point: 5.3292

>NTDB_id=524714 K5O18_RS08475 WP_046097579.1 1675135..1676559(+) (pilB) [Avibacterium paragallinarum strain p4chr1]
MAEYSLDEDDIVYAVDGKCFEITPHLWQQNKQQKDILIRYLAVPLQEDEHTLWLGIDDLTNLSACESFSFLTGKIVEPIL
LSEQDLKSLLQKLSPTLVEEPALLDFYQSSISPYSENQPEKLDEPIIQILNKVFESAMQLNASDIHLEPQNEQLQIRFRI
DGVLQHQPPISKQMSQRLISRLKLLARLDLSETRLPQDGRFHFKTTFSDILDFRISTLPTYYGEKAVLRLQKNKPVTLDF
SELGMTAIQRKTLQNALSQPQGLILVTGPTGSGKSITLYTALEWLNQSEKHILTAEDPIEIELPGIIQTQINPQIGLDFN
RLLRTFLRQDPDIIMLGEIRDAESAAIALRAAQTGHLVLSTLHTNNAHSAIARLLQLGIQQYEIESCLLLVIAQRLVRKK
CLHKTKNCECYQGYSGRTGIYQFLQAIFDSNTGQISYQTDYPSLWDSASHKIAQGITDIAELERVLGRHTKAEL

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=524714 K5O18_RS08475 WP_046097579.1 1675135..1676559(+) (pilB) [Avibacterium paragallinarum strain p4chr1]
ATGGCTGAGTATTCTTTAGACGAGGACGACATTGTATATGCTGTTGATGGAAAATGCTTTGAAATAACACCGCACTTATG
GCAACAAAATAAGCAGCAAAAAGATATTTTAATACGTTATTTAGCAGTACCTTTACAGGAAGATGAACATACACTTTGGC
TTGGTATTGATGACTTAACAAATCTATCTGCTTGTGAAAGTTTTTCATTTCTAACAGGAAAAATAGTTGAGCCGATTTTG
TTATCAGAACAAGATTTAAAATCGCTATTACAGAAGTTATCTCCCACATTAGTAGAAGAGCCGGCATTACTTGATTTTTA
TCAATCGTCTATTTCACCTTATAGCGAAAATCAACCAGAAAAACTTGATGAACCAATTATTCAAATACTAAATAAAGTTT
TTGAATCGGCAATGCAATTAAATGCCTCAGATATTCATTTAGAGCCACAGAATGAGCAATTGCAGATCCGTTTTCGTATT
GACGGGGTATTGCAACATCAGCCTCCTATTTCTAAACAAATGAGCCAACGTTTGATTTCTCGTTTAAAACTTCTAGCTCG
TCTTGATCTTAGCGAAACTCGCTTACCACAAGATGGAAGATTTCATTTCAAAACGACATTTTCCGATATATTAGACTTCC
GTATCTCGACATTACCTACATATTATGGAGAAAAAGCGGTACTACGTTTACAAAAAAATAAACCTGTTACCTTGGATTTT
TCTGAGCTGGGGATGACAGCGATACAACGAAAAACATTACAAAATGCTTTATCTCAACCTCAAGGACTCATTTTAGTAAC
GGGGCCAACTGGGAGTGGGAAAAGTATTACGCTCTATACAGCATTAGAATGGCTTAATCAATCGGAAAAACATATTCTAA
CAGCAGAAGATCCGATTGAAATAGAACTTCCAGGGATTATCCAAACGCAAATTAACCCACAAATTGGTTTAGATTTTAAC
CGTTTGTTGCGTACATTTTTACGTCAGGATCCAGACATTATTATGCTTGGTGAGATCCGTGATGCAGAAAGTGCAGCGAT
TGCGTTACGTGCAGCACAAACTGGACATTTAGTCCTTTCAACATTACATACAAATAATGCACATTCAGCTATTGCTCGCT
TGTTACAGCTTGGTATTCAACAATATGAAATCGAAAGTTGTTTATTATTAGTGATTGCCCAGCGTTTAGTCCGTAAAAAA
TGTCTACACAAAACGAAAAATTGCGAATGTTATCAAGGTTATAGTGGGCGTACTGGTATTTATCAATTTTTACAAGCTAT
TTTTGATTCAAATACAGGGCAAATTTCTTATCAAACCGATTATCCCTCATTATGGGATAGTGCTTCACATAAAATAGCAC
AAGGTATCACAGATATTGCTGAATTAGAACGAGTTTTAGGTAGGCATACAAAAGCTGAACTATAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(132-399)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F5F317

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

64.693

99.789

0.646
  pilB Haemophilus influenzae 86-028NP

63.924

100

0.639
  pilB Glaesserella parasuis strain SC1401

57.474

100

0.576
  pilB Legionella pneumophila strain ERS1305867

39.634

100

0.411
  pilB Vibrio campbellii strain DS40M4

40

100

0.409
  pilB Vibrio parahaemolyticus RIMD 2210633

39.794

100

0.407
  pilB Vibrio cholerae strain A1552

39.627

100

0.403
  pilB Acinetobacter baylyi ADP1

37.626

100

0.395
  pilF Neisseria gonorrhoeae MS11

37.317

100

0.376
  pilB Acinetobacter baumannii D1279779

36.495

100

0.373