Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K5O18_RS08470 Genome accession   NZ_CP081939
Coordinates   1674691..1675122 (+) Length   143 a.a.
NCBI ID   WP_035687024.1    Uniprot ID   A0A0F5ESJ8
Organism   Avibacterium paragallinarum strain p4chr1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1669691..1680122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5O18_RS08440 (K5O18_08440) - 1669847..1670698 (-) 852 WP_110479818.1 thymidylate synthase -
  K5O18_RS08445 (K5O18_08445) lgt 1670710..1671510 (-) 801 WP_017807046.1 prolipoprotein diacylglyceryl transferase -
  K5O18_RS08450 (K5O18_08450) - 1671520..1672311 (-) 792 WP_110479819.1 sulfite exporter TauE/SafE family protein -
  K5O18_RS08455 (K5O18_08455) rppH 1672314..1672907 (-) 594 WP_035687029.1 RNA pyrophosphohydrolase -
  K5O18_RS08460 (K5O18_08460) - 1673479..1673898 (-) 420 WP_133247373.1 hypothetical protein -
  K5O18_RS08465 (K5O18_08465) ampD 1673997..1674551 (-) 555 WP_110479836.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5O18_RS08470 (K5O18_08470) pilA 1674691..1675122 (+) 432 WP_035687024.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  K5O18_RS08475 (K5O18_08475) pilB 1675135..1676559 (+) 1425 WP_046097579.1 GspE/PulE family protein Machinery gene
  K5O18_RS08480 (K5O18_08480) pyrH 1676809..1677525 (+) 717 WP_035687020.1 UMP kinase -
  K5O18_RS08485 (K5O18_08485) frr 1677550..1678107 (+) 558 WP_017807054.1 ribosome recycling factor -
  K5O18_RS08490 (K5O18_08490) ispC 1678289..1679485 (+) 1197 WP_110479821.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15205.70 Da        Isoelectric Point: 9.8115

>NTDB_id=524713 K5O18_RS08470 WP_035687024.1 1674691..1675122(+) (pilA) [Avibacterium paragallinarum strain p4chr1]
MQNPTHLKIKKGFTLIELMIVIAIVAILATIAIPAYQNYTKKAAISELIQAASPYRSEVELCIYNTGATTKCNAGSNGIQ
KKLTNKGKVASIDVQKGTITVKGKDNLSDITYSLKASGDASKGVSWTVNCGKSVEFFPAGFCG

Nucleotide


Download         Length: 432 bp        

>NTDB_id=524713 K5O18_RS08470 WP_035687024.1 1674691..1675122(+) (pilA) [Avibacterium paragallinarum strain p4chr1]
ATGCAAAACCCAACCCATTTAAAAATCAAAAAAGGCTTTACTTTGATTGAACTGATGATCGTGATTGCGATTGTGGCGAT
TTTAGCGACTATTGCGATTCCTGCCTATCAAAATTACACCAAGAAAGCGGCAATATCCGAGCTAATTCAAGCCGCAAGCC
CCTATCGTTCAGAAGTGGAATTATGTATTTATAATACGGGGGCTACCACGAAATGTAATGCGGGTTCAAATGGAATTCAG
AAAAAATTAACGAATAAAGGGAAAGTGGCTTCTATTGATGTACAAAAAGGCACGATTACGGTTAAAGGAAAAGATAATCT
TTCTGATATTACTTATTCATTAAAAGCCAGTGGAGATGCCTCAAAAGGTGTATCTTGGACGGTAAATTGTGGAAAATCTG
TAGAGTTTTTCCCAGCGGGATTTTGCGGATAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(42-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F5ESJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

59.31

100

0.601
  pilA Haemophilus influenzae 86-028NP

57.931

100

0.587
  pilA Glaesserella parasuis strain SC1401

46.809

98.601

0.462
  pilA Vibrio campbellii strain DS40M4

34.615

100

0.378
  pilA Ralstonia pseudosolanacearum GMI1000

37.324

99.301

0.371