Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JHZ65_RS25265 Genome accession   NZ_CP067024
Coordinates   5651922..5654027 (-) Length   701 a.a.
NCBI ID   WP_200380594.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. maculicola strain MAFF 302723     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5646922..5659027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHZ65_RS25250 (JHZ65_25255) - 5648541..5650166 (-) 1626 WP_057418327.1 SPOR domain-containing protein -
  JHZ65_RS25255 (JHZ65_25260) aroB 5650177..5651280 (-) 1104 WP_005765495.1 3-dehydroquinate synthase -
  JHZ65_RS25260 (JHZ65_25265) aroK 5651399..5651917 (-) 519 WP_007245596.1 shikimate kinase AroK -
  JHZ65_RS25265 (JHZ65_25270) pilQ 5651922..5654027 (-) 2106 WP_200380594.1 type IV pilus secretin PilQ Machinery gene
  JHZ65_RS25270 (JHZ65_25275) pilP 5654075..5654602 (-) 528 WP_003378849.1 type 4a pilus biogenesis lipoprotein PilP -
  JHZ65_RS25275 (JHZ65_25280) pilO 5654599..5655222 (-) 624 WP_005765501.1 type 4a pilus biogenesis protein PilO -
  JHZ65_RS25280 (JHZ65_25285) - 5655219..5655833 (-) 615 WP_122389549.1 PilN domain-containing protein -
  JHZ65_RS25285 (JHZ65_25290) pilM 5655833..5656897 (-) 1065 WP_003378852.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75681.16 Da        Isoelectric Point: 5.0435

>NTDB_id=524546 JHZ65_RS25265 WP_200380594.1 5651922..5654027(-) (pilQ) [Pseudomonas syringae pv. maculicola strain MAFF 302723]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTAQPARIALDLPGVTSQLETKSRE
LGTGNARSVVVVQAQDRTRVIINLRTLAPYSSRVDGNNLFVVIGQGAAAAQASQPTTAMGAQRVAVPPPAPASARPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKAEERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSRNG
KWTTYGLDDNGDEGGNTNANLTSNIPFVDLGAADATTGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=524546 JHZ65_RS25265 WP_200380594.1 5651922..5654027(-) (pilQ) [Pseudomonas syringae pv. maculicola strain MAFF 302723]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATAGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCAGGCGACCGCATCGAGCTCAAGCTTGCCTTTGACGCACCGGTGCCCGCGCCTCGCG
GCTACACCACCGCGCAACCGGCGCGCATCGCTCTGGACCTGCCGGGCGTGACCAGCCAGCTGGAGACCAAAAGCCGTGAG
CTGGGCACCGGCAATGCACGCAGCGTAGTGGTGGTGCAGGCTCAGGATCGCACGCGAGTGATCATCAACCTGAGGACGCT
TGCGCCTTATAGCTCTCGCGTTGACGGCAACAATCTGTTTGTGGTCATCGGCCAGGGTGCCGCCGCAGCGCAGGCCTCCC
AGCCGACCACTGCGATGGGCGCGCAACGGGTAGCGGTGCCGCCGCCAGCCCCGGCGTCTGCCAGACCCTTTATACCGGCG
GGCGTCAAAGCCATCCGCAATGTCGATTTTCAGCGTGGTGAGCTGGGCGAAGGCAATGTGGTCATAGACCTGAGTAACCC
GAGCATCGCGCCTGATATTCAGGAGCAGGGCGGCAAGATCCGTGTCGACTTCGCCAAGACCCAGTTGCCCGAGCCTCTGC
GTGTGCGGCTGGACGTCAAGGATTTCGCGACCCCTGTGCAGTTCGTCAGTGCGTCGGCCACGGGCGACAAGGCCAGTATT
CTGATCGAGCCGTCGGGTGCCTTCGACTACTCGGCGTTCCAGACCGAAAACAAGCTGACCATCAGTGTGCGTCCGTTGAC
CAACGAAGATCTGGACCGCCGCGCCGCCGACAAGCCGGTGTACACCGGTGAAAAGCTCTCGCTTAACTTCCAGGACATCG
ATGTGCGTTCGGTGCTGCAACTGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGTGACACGGTTCAGGGCGGCATC
ACTCTGCGCCTGCAGAACGTGCCCTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGGT
CGGCAGTGTTCTGCTGGTTGCGCCAGCGGATGAAATTGCCGCCCGCGAACGCCAGGAGCTGGAATCGCTCAAGCAGATTG
CCGAACTGGCACCGCTGCGCCGCGAGCTGTTGCAGGTCAATTACGCCAAGGCCGCCGACATCGCCAAGCTGTTTCAGTCG
GTGACCAGTGCCGAGTCCAAGGCTGAAGAGCGTGGCTCGATCACCGTGGACGATCGCACCAACAACATCATTGCCTACCA
GACTCAGGACCGTCTCGACGAACTGCGTCGCATTGTTTCGCAACTGGATATTCCAGTGCGCCAGGTGATGATCGAGGCGC
GTATCGTCGAGGCCAACGTCGATTACAACAAGCAGTTGGGTGTGCGCTGGGGTGGCTCGACCAATACCAGTCGCAACGGT
AAATGGACCACCTACGGTCTGGACGACAATGGTGATGAGGGCGGTAACACGAATGCAAACCTGACCTCCAACATCCCTTT
TGTTGACCTGGGTGCAGCCGATGCGACCACAGGCATTGGCTTGGGCTTCGTAACCAACAACACGCTGCTGGACCTTGAGC
TGAGCGCGATGGAGAAGACCGGCAACGGTGAAATCGTCTCCCAGCCCAAAGTGGTCACATCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACAGAGATTCCGTATCAGGAATCCAGCTCAAGCGGCGCCACCACCGTGAGCTTCAAGGAGGCTTCGCT
GTCGCTTGAAGTCACGCCGCAGATCACGCCGGACAACCGCATCATTATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGCGTACCGCCCATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTGATCGGTGGTGTGTTCTCCAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.337

100

0.746
  comQ Acinetobacter baylyi ADP1

41.81

99.287

0.415
  pilQ Acinetobacter baumannii D1279779

38.639

100

0.405
  pilQ Acinetobacter baumannii strain A118

38.639

100

0.405
  pilQ Legionella pneumophila strain Lp02

37.951

98.859

0.375
  pilQ Legionella pneumophila strain ERS1305867

37.951

98.859

0.375