Detailed information NTG0524547

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: JHZ65_RS25285
• Genome accession: NZ_CP067024
• Coordinates: 5655833..5656897 (-)
• Length: 354 a.a.
• NCBI ID: WP_003378852.1
• Uniprot ID: A0A2G9KZJ9
• Organism: Pseudomonas syringae pv. maculicola strain MAFF 302723
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5650833..5661897

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JHZ65_RS25260 (JHZ65_25265)	aroK	5651399..5651917 (-)	519	WP_007245596.1	shikimate kinase AroK	-
JHZ65_RS25265 (JHZ65_25270)	pilQ	5651922..5654027 (-)	2106	WP_200380594.1	type IV pilus secretin PilQ	Machinery gene
JHZ65_RS25270 (JHZ65_25275)	pilP	5654075..5654602 (-)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
JHZ65_RS25275 (JHZ65_25280)	pilO	5654599..5655222 (-)	624	WP_005765501.1	type 4a pilus biogenesis protein PilO	-
JHZ65_RS25280 (JHZ65_25285)	-	5655219..5655833 (-)	615	WP_122389549.1	PilN domain-containing protein	-
JHZ65_RS25285 (JHZ65_25290)	pilM	5655833..5656897 (-)	1065	WP_003378852.1	pilus assembly protein PilM	Machinery gene
JHZ65_RS25290 (JHZ65_25295)	-	5657108..5659549 (+)	2442	WP_375232361.1	penicillin-binding protein 1A	-
JHZ65_RS25295 (JHZ65_25300)	-	5659751..5661019 (-)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38103.62 Da
• Isoelectric Point: 4.7008

>NTDB_id=524547 JHZ65_RS25285 WP_003378852.1 5655833..5656897(-) (pilM) [Pseudomonas syringae pv. maculicola strain MAFF 302723]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=524547 JHZ65_RS25285 WP_003378852.1 5655833..5656897(-) (pilM) [Pseudomonas syringae pv. maculicola strain MAFF 302723]
GTGTTCGAACTCTTTGGTAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGCTGTCTCGCGTACTGGTCAAGGCCAAGACCAATGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCTGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
CAAGCTTGAAGCTGATCAGTACATCCCTTACCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAAGTGCGCGAGGCCGCGCTGGCCCTG
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGTCTGCTGGCTGCGCAACTGGG
CAACGGCCATGACGAACTGACCGTTGCCGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCATGGTC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGTCGCCAGCTGACTGACGAAATTCAGCGCCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAGGTGCTGGACCCCTTCAAGGACGC
GCTGGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCGTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACACCGACCATGGTTGCCAACCCG
TTCGCCGACATGGCCTTGAGTTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCACCTGCCCTGATGATCGCGTGCGG
TCTGGCATTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2G9KZJ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469