Detailed information NTG0523581

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: JH279_RS16610
• Genome accession: NZ_CP066956
• Coordinates: 3785803..3787530 (-)
• Length: 575 a.a.
• NCBI ID: WP_274390759.1
• Uniprot ID: -
• Organism: Xanthomonas campestris pv. incanae strain 8-14
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3778299..3792963	3785803..3787530	within	0

Gene organization within MGE regions

Location: 3778299..3792963

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JH279_RS16575 (JH279_16520)	-	3778299..3778709 (-)	411	WP_323549302.1	DNA-binding protein	-
JH279_RS16580 (JH279_16525)	sucD	3778813..3779688 (-)	876	WP_011038208.1	succinate--CoA ligase subunit alpha	-
JH279_RS16585 (JH279_16530)	sucC	3779713..3780882 (-)	1170	WP_011038209.1	ADP-forming succinate--CoA ligase subunit beta	-
JH279_RS16590 (JH279_16535)	-	3781116..3782726 (+)	1611	WP_116901781.1	HAMP domain-containing sensor histidine kinase	-
JH279_RS16595 (JH279_16540)	pilR	3782935..3784329 (+)	1395	WP_274390757.1	sigma-54 dependent transcriptional regulator	Regulator
JH279_RS16600 (JH279_16545)	-	3784527..3784883 (+)	357	WP_139328384.1	hypothetical protein	-
JH279_RS16605 (JH279_16550)	-	3784883..3785620 (+)	738	WP_274390758.1	zeta toxin family protein	-
JH279_RS16610 (JH279_16555)	pilB	3785803..3787530 (-)	1728	WP_274390759.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JH279_RS16615 (JH279_16560)	-	3787608..3788420 (-)	813	WP_274390760.1	sterol desaturase family protein	-
JH279_RS16620 (JH279_16565)	comP	3788466..3788876 (-)	411	WP_274390761.1	pilin	Machinery gene
JH279_RS16625 (JH279_16570)	comP	3788960..3789400 (-)	441	WP_274390762.1	pilin	Machinery gene
JH279_RS16630 (JH279_16575)	pilC	3789701..3790957 (+)	1257	WP_274391840.1	type II secretion system F family protein	Machinery gene
JH279_RS16635 (JH279_16580)	-	3790964..3791827 (+)	864	WP_057671659.1	A24 family peptidase	-
JH279_RS16640 (JH279_16585)	coaE	3791841..3792464 (+)	624	WP_274390763.1	dephospho-CoA kinase	-

Sequence

Protein

• Length: 575 a.a.
• Molecular weight: 62535.81 Da
• Isoelectric Point: 5.7626

>NTDB_id=523581 JH279_RS16610 WP_274390759.1 3785803..3787530(-) (pilB) [Xanthomonas campestris pv. incanae strain 8-14]
MSMAANLVGITGIARRLVQDGALDEVVARAAMDQAAFAKVPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVFDASQN
AVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLGDD
DDEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVK
LSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNGETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPEH
ALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMKAS
HGVTSLAEINRVTKD

Nucleotide

• Length: 1728 bp

>NTDB_id=523581 JH279_RS16610 WP_274390759.1 3785803..3787530(-) (pilB) [Xanthomonas campestris pv. incanae strain 8-14]
ATGAGCATGGCAGCCAACCTAGTTGGTATTACTGGGATTGCTCGGCGCCTTGTCCAAGATGGCGCGTTGGACGAAGTCGT
TGCGCGCGCAGCGATGGATCAGGCAGCTTTCGCGAAAGTCCCTTTGCCTCAATGGTTTGCAGAGAAGAAGCTGGTCTCTG
CTGCTCAGCTTGCAGCCGCCAATGCAGTCGAGTTCGGTATGCCGCTGATGGATGTGTCGGTGTTTGACGCCAGCCAGAAT
GCGGTCAAGCTGGTCAGTGAGGAGTTGCTTCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGT
GGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTTCACACCAATTTGGTGGTCGAGCCGATTTTGG
TCGACGAGGACCAGATCCGTCGCACCTTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGCTCCTCGCTCGGCGACGAC
GATGATGAAATGGGGGATCTGGACGTCTCCACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGAGTCGATGCCAA
GGGCGATGACACGCCGGTGGTGAAATTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACATCCATT
TCGAGCCTTATGAAGATGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCGGTGAAG
CTCAGCCAGCGCATTGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGACGGGCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAAAAGGTGG
TACTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCGGACCAGCAGAAGCTGTTC
CTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATAC
CGCGCTCGGGATTCTCAACGGCGAGACGCGCAACATTTCCACGGCCGAGGATCCGGTCGAAATCCGGTTGCCCGGCGTCA
ATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACCTTTGCCGCTGCGCTGCGGTCGTTCCTGCGTCAGGACCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
GACACTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATCACCTCCT
CGGTGACCCTGGTCATCGCGCAGCGTTTGGCACGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCGGAACAT
GCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGAATGCAC
CGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGACCGATGAAATCGGTGCGATCGTGCTGGAAGGCG
GCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACTTGCGCCAGTCGGCGTTGATGAAGGCTTCG
CACGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (47-151)

• Type II/IV secretion system protein (T2SSE) (199-466)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	55.986	98.783	0.553
pilB	Acinetobacter baylyi ADP1	55.458	98.783	0.548
pilB	Legionella pneumophila strain ERS1305867	51.82	100	0.52
pilB	Vibrio cholerae strain A1552	48.354	100	0.485
pilF	Neisseria gonorrhoeae MS11	48.592	98.783	0.48
pilB	Vibrio campbellii strain DS40M4	45.87	98.957	0.454
pilB	Vibrio parahaemolyticus RIMD 2210633	45.234	100	0.454