Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   JFT56_RS17470 Genome accession   NZ_CP066369
Coordinates   4022762..4024027 (+) Length   421 a.a.
NCBI ID   WP_198781256.1    Uniprot ID   -
Organism   Shewanella putrefaciens strain CGMCC-1.6515     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4017762..4029027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFT56_RS17450 ampD 4017779..4018339 (-) 561 WP_198781252.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JFT56_RS17455 nadC 4018546..4019427 (+) 882 WP_198781253.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JFT56_RS17460 - 4020328..4020774 (+) 447 WP_198781254.1 pilin -
  JFT56_RS17465 pilB 4020867..4022576 (+) 1710 WP_198781255.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JFT56_RS17470 pilC 4022762..4024027 (+) 1266 WP_198781256.1 type II secretion system F family protein Machinery gene
  JFT56_RS17475 pilD 4024319..4025260 (+) 942 WP_198781257.1 prepilin peptidase Machinery gene
  JFT56_RS17480 coaE 4025542..4026159 (+) 618 WP_198781258.1 dephospho-CoA kinase -
  JFT56_RS17485 zapD 4026152..4026886 (+) 735 WP_198781259.1 cell division protein ZapD -
  JFT56_RS17490 yacG 4026966..4027175 (+) 210 WP_198781260.1 DNA gyrase inhibitor YacG -
  JFT56_RS17495 mutT 4027215..4027628 (+) 414 WP_198781261.1 8-oxo-dGTP diphosphatase MutT -
  JFT56_RS17500 - 4027879..4028415 (+) 537 WP_198781262.1 thioredoxin family protein -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46186.52 Da        Isoelectric Point: 10.1516

>NTDB_id=519493 JFT56_RS17470 WP_198781256.1 4022762..4024027(+) (pilC) [Shewanella putrefaciens strain CGMCC-1.6515]
MATATTLKKPRAKKTEQKGQPKIYTFEWKGVNRDGAKTGGELKGATVAEIKSQLKSQGVNPKVVRKKSAPFFARNPDIKP
MDIAMVTRQIATMLAAGVPLVTTIEMLGRGHEKLKMRELLGTVLSEIQSGIPLSDALRPHRRYFDDLYVDLVAAGEHSGS
LDAVFDRIATYREKAEQLKSKIKKAMFYPAAVVVVAVAVTVLLLLFVVPQFEEIFKGFGAELPAFTQLIIGISRFLQSSW
YFFFAAIVISIWLFVRAHRNSQALRDRVDELVLKIPIIGEILHKAAMARFSRTLATTFAAGVPLIDGMESAAGASGNAVY
RKALLKVRQEVMAGMQMNVAMRTTGLFPDMLIQMVMIGEESGSLDNMLNKVANIYEMQVDDAVDGLSSLIEPIMMVVIGT
LVGGLIVGMYLPIFQMGNVVG

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=519493 JFT56_RS17470 WP_198781256.1 4022762..4024027(+) (pilC) [Shewanella putrefaciens strain CGMCC-1.6515]
ATGGCTACGGCAACGACATTAAAAAAGCCCCGCGCCAAAAAGACGGAGCAAAAAGGCCAACCGAAAATTTATACCTTTGA
ATGGAAAGGGGTAAACCGCGATGGCGCCAAAACTGGCGGCGAACTCAAGGGCGCCACAGTTGCCGAAATAAAAAGCCAGC
TTAAATCCCAGGGTGTTAACCCTAAAGTAGTGCGTAAAAAATCCGCACCCTTCTTTGCCCGTAATCCCGATATTAAACCT
ATGGATATCGCTATGGTAACTCGCCAAATTGCCACTATGTTGGCGGCGGGCGTTCCCCTAGTGACCACCATAGAAATGTT
AGGTCGCGGTCATGAAAAACTAAAGATGCGTGAACTCCTAGGCACTGTGCTTTCGGAAATCCAATCGGGTATTCCATTAT
CGGACGCGCTGCGCCCCCATAGGCGTTATTTCGATGATTTATATGTCGACTTAGTCGCAGCGGGTGAGCACTCGGGCTCA
CTCGATGCGGTATTCGATCGTATCGCCACCTATAGGGAAAAAGCCGAGCAGTTAAAATCGAAAATTAAAAAAGCCATGTT
CTACCCCGCAGCCGTAGTTGTAGTCGCAGTTGCCGTTACTGTACTATTACTGCTCTTTGTGGTACCGCAATTTGAAGAAA
TTTTTAAGGGTTTTGGCGCCGAACTCCCCGCCTTCACTCAACTAATTATTGGCATCTCGCGTTTTTTACAGAGCTCTTGG
TATTTTTTCTTCGCAGCCATTGTCATCAGTATCTGGTTATTTGTGCGCGCCCACCGTAACTCACAGGCCCTTCGCGACCG
CGTCGATGAGCTAGTGCTTAAAATCCCCATTATCGGCGAGATATTGCATAAAGCGGCTATGGCACGATTTTCCCGCACCT
TAGCCACTACCTTTGCCGCTGGGGTGCCGCTTATCGATGGCATGGAATCCGCCGCGGGTGCATCCGGCAATGCGGTCTAC
CGCAAAGCCCTGCTTAAAGTGCGCCAAGAAGTGATGGCGGGGATGCAGATGAACGTCGCCATGCGCACCACTGGGCTTTT
CCCCGACATGCTGATCCAAATGGTGATGATAGGCGAAGAGTCAGGCTCACTAGATAATATGCTCAATAAGGTCGCTAATA
TTTATGAGATGCAGGTGGATGATGCAGTCGATGGCCTATCGAGCCTAATTGAGCCCATTATGATGGTGGTGATTGGCACA
CTCGTAGGTGGCCTAATTGTGGGTATGTACCTGCCTATCTTCCAAATGGGAAATGTCGTAGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-209)

Type II secretion system (T2SS), protein F (T2SSF)

(290-412)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.937

93.824

0.515
  pilC Acinetobacter baylyi ADP1

50.63

94.299

0.477
  pilC Legionella pneumophila strain ERS1305867

48.762

95.962

0.468
  pilC Acinetobacter baumannii D1279779

49.497

94.537

0.468
  pilC Vibrio cholerae strain A1552

46.985

94.537

0.444
  pilC Vibrio campbellii strain DS40M4

44.169

95.724

0.423
  pilG Neisseria meningitidis 44/76-A

40.547

95.487

0.387
  pilG Neisseria gonorrhoeae MS11

40.547

95.487

0.387