Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GKQ51_RS03475 Genome accession   NZ_CP066310
Coordinates   720983..723100 (+) Length   705 a.a.
NCBI ID   WP_198867245.1    Uniprot ID   -
Organism   Azotobacter chroococcum strain HR1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 715983..728100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKQ51_RS03455 (GKQ51_03455) comM 718067..719131 (+) 1065 WP_198867243.1 pilus assembly protein PilM Machinery gene
  GKQ51_RS03460 (GKQ51_03460) - 719132..719788 (+) 657 WP_198867244.1 PilN domain-containing protein -
  GKQ51_RS03465 (GKQ51_03465) pilO 719785..720408 (+) 624 WP_089167131.1 type 4a pilus biogenesis protein PilO -
  GKQ51_RS03470 (GKQ51_03470) pilP 720405..720929 (+) 525 WP_089167132.1 type 4a pilus biogenesis lipoprotein PilP -
  GKQ51_RS03475 (GKQ51_03475) pilQ 720983..723100 (+) 2118 WP_198867245.1 type IV pilus secretin PilQ Machinery gene
  GKQ51_RS03480 (GKQ51_03480) aroK 723105..723623 (+) 519 WP_198867246.1 shikimate kinase AroK -
  GKQ51_RS03485 (GKQ51_03485) aroB 723675..724778 (+) 1104 WP_198867247.1 3-dehydroquinate synthase -
  GKQ51_RS03490 (GKQ51_03490) - 724786..726450 (+) 1665 WP_198867248.1 AAA family ATPase -

Sequence

Protein


Download         Length: 705 a.a.        Molecular weight: 75952.17 Da        Isoelectric Point: 4.6350

>NTDB_id=519240 GKQ51_RS03475 WP_198867245.1 720983..723100(+) (pilQ) [Azotobacter chroococcum strain HR1]
MNSTLSRLGISVLAALLSPELLAADLKALDVAALPGDRVELKLSFDEPVPAPRGYTIEQPARIALDLPGVANKLGTRYRE
LGVGNARSVTLVEAGGRTRMIVNLASLSPYSTRAEGNNLYVVIGESAAAPRQAGTAPSPVVTAAPVPSVKPYAPAGKTVQ
GIDFQRGEQGEGNVIINLSSPGMNPDVQEQGGKIRLDFPGAQLPESLRARLDVKDFATPVQFVSASSEGGKASILIEPTG
MYDYLVYQTDNRLTVSVKPLTQENVAKRNADRFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGNITLRLQN
VPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQQQLAELAPLRRELVQVNYAKAADIAKLFQSVTSGEE
GEEQRGSITVDERTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSFVNDQWSLYGKDG
AQSIDDGQVFLPGTDQVGNFTLEDGVAPVPFVDMGVANSTSGLGIGFVSDHLILDLQLSAMEKTGNGEVISQPKVLTSDK
ETAKILTGEEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDQADFSKAIGGVPPINKNEVNAKVLIG
DGETIVIGGVFSNTQAKSVDKVPFLGDLPFIGRLFRRDVVADSKSELLVFLTPRILNNQAIAVSR

Nucleotide


Download         Length: 2118 bp        

>NTDB_id=519240 GKQ51_RS03475 WP_198867245.1 720983..723100(+) (pilQ) [Azotobacter chroococcum strain HR1]
ATGAACAGCACCCTTTCGCGTCTTGGTATTTCAGTCCTGGCTGCCCTGCTGTCGCCGGAATTGTTGGCGGCCGACCTCAA
GGCTCTGGATGTGGCCGCCCTGCCTGGCGATCGGGTCGAGCTCAAGTTGTCCTTCGACGAGCCCGTGCCGGCGCCGCGCG
GCTATACCATCGAGCAGCCGGCCCGGATTGCCCTGGATCTGCCCGGTGTAGCCAACAAGCTGGGGACCCGGTATCGCGAG
CTGGGCGTCGGCAATGCCCGCAGCGTCACCCTGGTCGAGGCGGGCGGCCGCACCCGGATGATCGTCAACCTGGCGAGCCT
GTCGCCCTATAGCACGCGAGCCGAAGGCAACAACCTCTATGTGGTGATCGGCGAGTCGGCCGCCGCGCCCCGGCAGGCCG
GCACTGCGCCGTCACCGGTCGTCACGGCGGCTCCGGTTCCGTCGGTCAAGCCCTATGCGCCGGCCGGCAAGACCGTCCAG
GGCATCGACTTCCAGCGCGGCGAGCAGGGCGAAGGCAACGTTATCATCAATCTGTCGAGTCCGGGCATGAATCCCGATGT
CCAGGAGCAGGGTGGCAAGATCCGCCTGGACTTCCCGGGTGCGCAGTTGCCGGAATCCCTGCGGGCACGTCTGGATGTCA
AGGATTTCGCTACACCCGTGCAGTTCGTGAGTGCTTCGAGTGAGGGTGGCAAGGCCAGCATTCTGATTGAACCGACCGGC
ATGTACGACTATCTGGTCTACCAGACCGACAACCGCCTGACCGTCAGCGTCAAGCCGCTGACCCAGGAAAATGTTGCCAA
GCGCAATGCCGACCGCTTCGCCTATACCGGCGAGAAGCTGTCGCTGAACTTCCAGGATATCGATGTCCGCTCGGTCCTGC
AGCTGATCGCCGACTTCACCAACCTCAACCTGGTGGCCAGCGACACCGTGCAGGGCAATATCACCCTGCGCCTGCAGAAT
GTGCCCTGGGACCAGGCGCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTCGGCAATGTCCTGCTGGT
GGCGCCGGCCGACGAAATCGCGGCGCGCGAGCGTCAGGAGCTGGAGGCGCAGCAGCAGCTCGCCGAGCTGGCCCCCCTGC
GCCGCGAGCTGGTCCAGGTGAACTACGCCAAGGCAGCGGATATCGCCAAGCTGTTCCAATCGGTCACCAGCGGCGAAGAG
GGTGAAGAGCAGCGCGGTTCCATCACCGTCGACGAGCGGACCAACAACATCATCGCCTACCAGACCCAGGAGCGTCTGGA
CGAGCTGCGACGGATCGTCGCCCAGCTGGACATTCCGGTGCGCCAGGTGATGATCGAGGCACGCATCGTCGAGGCCAATG
TCGATTACGACAAGAGCCTGGGGGTGCGCTGGGGCGGCAGCTTCGTGAACGACCAGTGGAGCCTGTATGGCAAGGATGGT
GCGCAATCCATCGATGACGGGCAGGTGTTTCTGCCCGGGACCGATCAGGTCGGCAACTTCACCCTGGAGGATGGCGTGGC
GCCGGTCCCCTTCGTCGACATGGGCGTGGCGAACAGTACATCGGGCCTTGGCATCGGCTTCGTTTCCGACCACCTGATTC
TCGACCTTCAACTGTCGGCCATGGAAAAGACCGGCAATGGAGAGGTCATTTCCCAGCCCAAGGTCCTCACTTCGGACAAG
GAGACGGCGAAGATCCTGACCGGCGAGGAGATCCCCTACCAGGAGGCCAGCTCCAGCGGTGCGACCAGCACCTCCTTCAA
GGAGGCGGCGCTGTCGCTGGAGGTGACGCCGCAGATCACCCCCGACAACCGCATCATCATGGAGGTGAAGGTGACCAAGG
ATCAGGCGGACTTCTCCAAGGCGATAGGCGGAGTGCCGCCGATCAACAAGAACGAGGTCAACGCCAAGGTGCTGATCGGC
GACGGCGAAACCATCGTGATCGGCGGAGTCTTCTCCAATACCCAGGCCAAGTCCGTGGACAAGGTGCCCTTCCTCGGCGA
CCTGCCGTTCATCGGACGTCTGTTCCGCCGCGACGTGGTCGCCGACAGCAAATCCGAGCTGCTTGTTTTCCTGACTCCGC
GTATTCTTAACAACCAGGCTATTGCGGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(540-696)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.035

100

0.763
  comQ Acinetobacter baylyi ADP1

42.468

98.865

0.42
  pilQ Acinetobacter baumannii strain A118

40.506

100

0.409
  pilQ Acinetobacter baumannii D1279779

40.506

100

0.409
  pilQ Legionella pneumophila strain ERS1305867

37.814

96.028

0.363
  pilQ Legionella pneumophila strain Lp02

37.518

96.028

0.36