Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   KZ795_RS02950 Genome accession   NZ_CP080286
Coordinates   653494..654117 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain UNC_PaerCF12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 648494..659117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZ795_RS02930 (KZ795_02930) aroB 649089..650195 (-) 1107 WP_033977028.1 3-dehydroquinate synthase -
  KZ795_RS02935 (KZ795_02935) aroK 650245..650763 (-) 519 WP_003095833.1 shikimate kinase AroK -
  KZ795_RS02940 (KZ795_02940) pilQ 650775..652919 (-) 2145 WP_033977030.1 type 4a pilus secretin PilQ Machinery gene
  KZ795_RS02945 (KZ795_02945) comL/pilP 652973..653497 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  KZ795_RS02950 (KZ795_02950) pilO 653494..654117 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  KZ795_RS02955 (KZ795_02955) pilN 654114..654710 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  KZ795_RS02960 (KZ795_02960) pilM 654710..655774 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  KZ795_RS02965 (KZ795_02965) - 655959..658430 (+) 2472 WP_039026606.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=519140 KZ795_RS02950 WP_003103268.1 653494..654117(-) (pilO) [Pseudomonas aeruginosa strain UNC_PaerCF12]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=519140 KZ795_RS02950 WP_003103268.1 653494..654117(-) (pilO) [Pseudomonas aeruginosa strain UNC_PaerCF12]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGAGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401