Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   I9H07_RS01455 Genome accession   NZ_CP066265
Coordinates   312992..315070 (+) Length   692 a.a.
NCBI ID   WP_236425136.1    Uniprot ID   -
Organism   Pseudomonas syringae strain PA-2-9E     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 307992..320070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H07_RS01435 (I9H07_01420) pilM 310161..311225 (+) 1065 WP_024674928.1 pilus assembly protein PilM Machinery gene
  I9H07_RS01440 (I9H07_01425) - 311225..311800 (+) 576 WP_024646247.1 PilN domain-containing protein -
  I9H07_RS01445 (I9H07_01430) pilO 311797..312420 (+) 624 WP_024674927.1 type 4a pilus biogenesis protein PilO -
  I9H07_RS01450 (I9H07_01435) pilP 312417..312944 (+) 528 WP_236425137.1 type 4a pilus biogenesis lipoprotein PilP -
  I9H07_RS01455 (I9H07_01440) pilQ 312992..315070 (+) 2079 WP_236425136.1 type IV pilus secretin PilQ Machinery gene
  I9H07_RS01460 (I9H07_01445) aroK 315075..315593 (+) 519 WP_024674924.1 shikimate kinase AroK -
  I9H07_RS01465 (I9H07_01450) aroB 315710..316813 (+) 1104 WP_024674923.1 3-dehydroquinate synthase -
  I9H07_RS01470 (I9H07_01455) - 316824..318443 (+) 1620 WP_024674922.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74353.57 Da        Isoelectric Point: 5.0971

>NTDB_id=518991 I9H07_RS01455 WP_236425136.1 312992..315070(+) (pilQ) [Pseudomonas syringae strain PA-2-9E]
MTRILSIIGASLCIAMLSPVVQAATLKTLDVAALPGDRIELKLSFDAPVPAPRGYTTAQPARIALDLPGVTSQLETKSRE
LGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGATAAQASQPSTAMGASRVAVPPPTPASARPFVPA
GAKAIRNIDFQRGELGEGNVVIDLSNANIAPDIQEQGGKIRVDFAKTLLPEPLRVRLDVKDFATPVQFVSASAAGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTHEDMDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKIGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKTDERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKELGVRWGGATNTSGSS
NWSTGTSTGGSTANSSTFVDMGAANATSGIGLGFLTNNTMLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEVPY
QESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYVNAVLGVPPIKKNEVNAKVLISDGETIVIGGVFSN
TQSKSVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=518991 I9H07_RS01455 WP_236425136.1 312992..315070(+) (pilQ) [Pseudomonas syringae strain PA-2-9E]
ATGACCCGGATTCTCTCGATTATCGGCGCGTCGTTATGCATCGCGATGCTGTCTCCGGTTGTCCAGGCGGCCACCCTCAA
GACCCTGGATGTCGCCGCGTTGCCGGGTGACCGTATCGAACTCAAGCTGTCGTTCGACGCACCGGTGCCAGCCCCGCGTG
GCTACACCACTGCGCAACCGGCGCGTATCGCGCTGGACCTGCCCGGGGTGACCAGCCAGCTGGAGACCAAGAGTCGTGAG
CTGGGCACCGGCAACGCGCGCAGCGTTGTGGTGGTGCAGGCTCAGGATCGCACGCGGGTGATCATCAACCTGACGACGCT
GTCGCCTTACAGCTCTCGGGTCGACGGTAACAATCTGTTCGTGGTCATCGGTCAGGGCGCAACCGCGGCCCAGGCTTCCC
AGCCAAGCACCGCGATGGGCGCATCCCGAGTGGCAGTGCCACCGCCCACGCCTGCGTCCGCCAGACCTTTTGTCCCTGCC
GGTGCCAAGGCCATCAGGAATATCGACTTTCAGCGCGGCGAGCTGGGCGAAGGCAACGTGGTCATCGACCTGAGCAACGC
CAATATTGCGCCTGATATTCAGGAGCAGGGCGGCAAGATTCGCGTGGACTTCGCCAAGACATTGCTGCCCGAGCCGTTGC
GCGTCCGGCTCGACGTCAAGGACTTCGCAACGCCGGTGCAGTTCGTCAGTGCTTCGGCCGCAGGCGACAAAGCGAGCATT
CTGATCGAGCCGTCGGGCGCATTCGACTACTCGGCGTTTCAGACCGAAAACAAGCTGACCATCAGTGTGCGTCCGCTGAC
CCACGAAGACATGGACCGGCGTGCCGCCGACAAGCCGGTGTATACCGGCGAAAAGCTCTCGCTGAATTTCCAGGACATTG
ACGTGCGCTCGGTGCTGCAACTGATCGCCGATTTCACCAACCTCAATCTGGTCGCCAGTGACACGGTTCAGGGCGGCATC
ACCCTGCGCCTGCAGAACGTGCCCTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGAT
CGGCAGCGTCCTGCTGGTCGCCCCGGCCGATGAAATTGCCGCCCGCGAACGTCAGGAACTGGAATCGCTCAAGCAGATCG
CCGAGCTGGCGCCGTTGCGTCGCGAGCTGTTGCAGGTCAACTACGCCAAGGCTGCTGACATCGCCAAGCTGTTCCAGTCG
GTGACCAGTGCCGAGTCCAAGACGGACGAGCGTGGCTCGATCACGGTCGATGACCGGACCAACAACATCATTGCCTACCA
GACCCAGGACCGTCTCGACGAGCTGCGGCGCATCGTTTCGCAGCTGGATATTCCGGTGCGTCAGGTGATGATCGAAGCGC
GCATCGTTGAGGCCAACGTCGATTACGACAAAGAGCTGGGGGTGCGCTGGGGTGGCGCGACGAACACCAGCGGCAGCAGC
AACTGGTCGACGGGCACCTCAACGGGCGGCTCCACGGCCAACAGCTCAACGTTTGTGGATATGGGCGCTGCCAACGCCAC
CTCCGGAATCGGCCTTGGCTTCCTGACCAACAACACGATGCTGGACCTTGAGTTGACTGCGATGGAAAAGACCGGCAACG
GTGAAATCGTCTCCCAGCCCAAGGTGGTCACGTCCGACAAGGAAACCGCCAAGATCCTCAAGGGCACCGAGGTTCCGTAT
CAGGAATCCAGCTCCAGCGGCGCCACCACCGTCAGCTTCAAGGAAGCGTCTCTGTCGCTTGAAGTCACCCCGCAGATTAC
CCCCGACAACCGCATCATCATGGAGGTCAAAGTCACCAAGGATGAGCCCGATTACGTGAATGCGGTGCTGGGCGTCCCGC
CGATCAAGAAAAACGAAGTCAACGCCAAAGTGCTGATTTCCGACGGTGAAACCATCGTGATTGGTGGTGTGTTCTCGAAT
ACGCAAAGTAAAAGTGTGGATAAAGTGCCATTTCTTGGCGATGTACCGTATCTTGGCCGGCTTTTCCGGCGCGATGTGGT
ATCCGAGTCCAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACAACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Secretin and TonB N terminus short domain (STN)

(309-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.361

100

0.753
  comQ Acinetobacter baylyi ADP1

42.014

100

0.422
  pilQ Acinetobacter baumannii strain A118

38.859

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.859

100

0.413
  pilQ Legionella pneumophila strain ERS1305867

37.628

98.699

0.371
  pilQ Legionella pneumophila strain Lp02

37.628

98.699

0.371