Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   I9H07_RS01435 Genome accession   NZ_CP066265
Coordinates   310161..311225 (+) Length   354 a.a.
NCBI ID   WP_024674928.1    Uniprot ID   -
Organism   Pseudomonas syringae strain PA-2-9E     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 305161..316225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H07_RS01425 (I9H07_01410) - 305586..306854 (+) 1269 WP_024672916.1 malic enzyme-like NAD(P)-binding protein -
  I9H07_RS01430 (I9H07_01415) - 307510..309954 (-) 2445 WP_024674929.1 penicillin-binding protein 1A -
  I9H07_RS01435 (I9H07_01420) pilM 310161..311225 (+) 1065 WP_024674928.1 pilus assembly protein PilM Machinery gene
  I9H07_RS01440 (I9H07_01425) - 311225..311800 (+) 576 WP_024646247.1 PilN domain-containing protein -
  I9H07_RS01445 (I9H07_01430) pilO 311797..312420 (+) 624 WP_024674927.1 type 4a pilus biogenesis protein PilO -
  I9H07_RS01450 (I9H07_01435) pilP 312417..312944 (+) 528 WP_236425137.1 type 4a pilus biogenesis lipoprotein PilP -
  I9H07_RS01455 (I9H07_01440) pilQ 312992..315070 (+) 2079 WP_236425136.1 type IV pilus secretin PilQ Machinery gene
  I9H07_RS01460 (I9H07_01445) aroK 315075..315593 (+) 519 WP_024674924.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38045.59 Da        Isoelectric Point: 4.7559

>NTDB_id=518990 I9H07_RS01435 WP_024674928.1 310161..311225(+) (pilM) [Pseudomonas syringae strain PA-2-9E]
MFGLFNKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=518990 I9H07_RS01435 WP_024674928.1 310161..311225(+) (pilM) [Pseudomonas syringae strain PA-2-9E]
GTGTTCGGACTCTTCAACAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGCTCCGGCACCCGTTACAAGGTCGAGTCCTACGCAGTCGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGCCAGGCGCTGTCTCGCGTACTGGTAAAGGCCAAGACCAGTGTCAAGATTGTGGCGGTG
GCAGTGGCCGGTTCTGCGGTGATCACCAAAACCATCGAGATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTCGAAGCCGACCAGTACATTCCTTATCCGCTTGAAGAAGTCGCCATCGATTTTGAAGTGCAGGGCTACTCGGTTC
GCAACCCCGAGCGTGTCGAGGTCCTGCTGGCGGCTTGCCGCAAGGAAAACGTCGAAGTACGCGAAGCTGCACTGGCGCTG
GCCGGGTTGACGGCGCGCGTCGTGGACGTTGAGGCCTATGCGCTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAACTGGG
CAACGGTCACGACGAACTGACTGTGGCCGTGGTGGACATTGGCGCGACCATGACCACCCTGAGCGTGCTGCACCATGGCC
GGATCATCTATACCCGCGAGCAACTGTTCGGCGGCCGCCAATTGACTGACGAGATTCAGCGCCGCTATGGCCTGTCCATG
GAAGAGGCCGGGCTCGCCAAGAAACAGGGCGGGCTGCCCGACGATTATGTCAGCGAAGTGCTCGACCCGTTCAAGGATGC
ACTGGTGCAACAGGTCTCTCGCTCGTTGCAGTTCTTTTTTGCAGCCGGGCAATACAACTCGGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCCATTTCCGGGCTGGAGCATCTGATCCAGCGACGCATCGGCACGCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCTTGAGCGCCAAGGTCAACGCCGGAGCACTGGCCAGTGACGCCCCGGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.761

100

0.469