Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   I9H08_RS01660 Genome accession   NZ_CP066263
Coordinates   362966..365071 (+) Length   701 a.a.
NCBI ID   WP_024639377.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae strain Pss9644     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 357966..370071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H08_RS01640 (I9H08_01635) comM 360135..361199 (+) 1065 WP_003403040.1 pilus assembly protein PilM Machinery gene
  I9H08_RS01645 (I9H08_01640) - 361199..361774 (+) 576 WP_003403038.1 PilN domain-containing protein -
  I9H08_RS01650 (I9H08_01645) pilO 361771..362394 (+) 624 WP_003403037.1 type 4a pilus biogenesis protein PilO -
  I9H08_RS01655 (I9H08_01650) pilP 362391..362918 (+) 528 WP_103360066.1 type 4a pilus biogenesis lipoprotein PilP -
  I9H08_RS01660 (I9H08_01655) pilQ 362966..365071 (+) 2106 WP_024639377.1 type IV pilus secretin PilQ Machinery gene
  I9H08_RS01665 (I9H08_01660) aroK 365076..365594 (+) 519 WP_003403034.1 shikimate kinase AroK -
  I9H08_RS01670 (I9H08_01665) aroB 365709..366812 (+) 1104 WP_003403032.1 3-dehydroquinate synthase -
  I9H08_RS01675 (I9H08_01670) - 366823..368445 (+) 1623 WP_011266379.1 AAA family ATPase -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75361.75 Da        Isoelectric Point: 5.0294

>NTDB_id=518962 I9H08_RS01660 WP_024639377.1 362966..365071(+) (pilQ) [Pseudomonas syringae pv. syringae strain Pss9644]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRD
LGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAVPPPTPASAKPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPGIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGSG
KWTTYGLDNNGDEAGNTGSSVTSNVPFVDLGAADATTGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=518962 I9H08_RS01660 WP_024639377.1 362966..365071(+) (pilQ) [Pseudomonas syringae pv. syringae strain Pss9644]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGTGACCGCATCGAGCTGAAGCTGGCATTCGACGCCCCGGTGCCTGCACCGCGCG
GTTACACCACCTCGCAACCGGCGCGCATTGCGCTGGACCTGCCGGGTGTTACCAGCCAGCTGGCGACCAAAAGTCGTGAC
CTGGGCACCGGCAATGCGCGCAGTGTGGTCGTGGTACAGGCTCAGGACCGTACCCGGGTGATCATCAACCTGACCACGCT
TTCGCCGTACAGCTCCCGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGCCAGGGTGCCGGCGCCGCGCAAGCCTCCC
AGCCGACTACCGCGATGGGCGCGCAACGGGTCGCGGTGCCACCGCCCACACCGGCATCCGCCAAGCCGTTCATCCCCGCT
GGCGTCAAGGCCATCAGGAATGTCGACTTCCAGCGTGGCGAGCTGGGTGAGGGCAATGTGGTCATCGACCTGAGCAACCC
CGGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTGGACTTCGCCAAGACCCAGCTGCCCGAACCGCTGC
GCGTCCGCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAGTGCTTCGGCGACAGGTGACAAGGCCAGCATT
CTGATCGAGCCGTCGGGCGCCTTCGACTACTCGGCGTTCCAGACCGAAAACAAGCTGACGATCAGCGTGCGCCCGTTGAC
CAACGAGGATCTGGACCGCCGCGCTGCCGACAAGCCGGTGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ATGTGCGTTCGGTGCTGCAGCTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGTGATACGGTCCAGGGCGGTATC
ACCCTGCGTCTGCAGAATGTGCCATGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGT
CGGCAACGTTCTACTGGTCGCACCGGCTGATGAAATTGCCGCCCGCGAGCGTCAGGAGCTGGAGTCACTCAAACAGATCG
CCGAGCTGGCACCCCTGCGCCGTGAGCTGCTGCAGGTCAATTATGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAGTCC
GTGACCAGTGCCGAGTCCAAGGCCGACGAGCGTGGCTCGATCACGGTGGACGACCGGACCAACAACATCATTGCCTACCA
GACCCAGGAACGACTCGACGAACTGCGTCGTATCGTGTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGCTCGGGGTACGCTGGGGCGGGTCTACCAACACCAGCGGCAGCGGC
AAATGGACAACCTACGGGCTGGATAATAACGGCGACGAGGCCGGTAACACTGGCAGCAGTGTGACCTCCAACGTGCCGTT
CGTGGACCTGGGCGCTGCTGATGCGACGACCGGCATAGGCCTGGGCTTCGTGACCAACAACACGCTGCTGGACCTTGAGC
TGAGTGCCATGGAGAAAACCGGTAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACGGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCGACCACCGTGAGCTTCAAGGAAGCTTCCCT
GTCGCTTGAAGTGACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAAGTGAAGGTCACCAAGGACGAGCCGGACT
ACCTGAATGCGGTACTGGGCGTACCGCCCATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAAACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.232

100

0.753
  comQ Acinetobacter baylyi ADP1

42.241

99.287

0.419
  pilQ Acinetobacter baumannii strain A118

38.912

100

0.408
  pilQ Acinetobacter baumannii D1279779

38.912

100

0.408
  pilQ Legionella pneumophila strain ERS1305867

38.696

98.431

0.381
  pilQ Legionella pneumophila strain Lp02

38.696

98.431

0.381