Detailed information NTG0518961

Bioinformatically predicted

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: I9H08_RS01640
• Genome accession: NZ_CP066263
• Coordinates: 360135..361199 (+)
• Length: 354 a.a.
• NCBI ID: WP_003403040.1
• Uniprot ID: A0AAJ4E2D4
• Organism: Pseudomonas syringae pv. syringae strain Pss9644
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 355135..366199

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I9H08_RS01630 (I9H08_01625)	-	355965..357233 (+)	1269	WP_011266374.1	malic enzyme-like NAD(P)-binding protein	-
I9H08_RS01635 (I9H08_01630)	-	357484..359928 (-)	2445	WP_032655050.1	penicillin-binding protein 1A	-
I9H08_RS01640 (I9H08_01635)	comM	360135..361199 (+)	1065	WP_003403040.1	pilus assembly protein PilM	Machinery gene
I9H08_RS01645 (I9H08_01640)	-	361199..361774 (+)	576	WP_003403038.1	PilN domain-containing protein	-
I9H08_RS01650 (I9H08_01645)	pilO	361771..362394 (+)	624	WP_003403037.1	type 4a pilus biogenesis protein PilO	-
I9H08_RS01655 (I9H08_01650)	pilP	362391..362918 (+)	528	WP_103360066.1	type 4a pilus biogenesis lipoprotein PilP	-
I9H08_RS01660 (I9H08_01655)	pilQ	362966..365071 (+)	2106	WP_024639377.1	type IV pilus secretin PilQ	Machinery gene
I9H08_RS01665 (I9H08_01660)	aroK	365076..365594 (+)	519	WP_003403034.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38093.54 Da
• Isoelectric Point: 4.7049

>NTDB_id=518961 I9H08_RS01640 WP_003403040.1 360135..361199(+) (comM) [Pseudomonas syringae pv. syringae strain Pss9644]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKNVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=518961 I9H08_RS01640 WP_003403040.1 360135..361199(+) (comM) [Pseudomonas syringae pv. syringae strain Pss9644]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCTCGCGTGCTCGCCAAGGCCAAGACCAGCGTCAAGAACGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTCGCTATCGATTTCGAAGTGCAGGGCTATTCGGTGC
GCAATCCCGAGCGCGTCGAGGTGTTGCTGGCTGCCTGCCGCAAGGAGAACGTCGAAGTGCGCGAGGCCGCACTGGCGCTG
GCCGGGCTGACCGCGCGCGTGGTCGATGTCGAGGCCTACGCGCTTGAGCGCTCGTTCGGACTGCTGGCCGCGCAACTGGG
CAACGGTCATGACGAGCTGACGGTGGCCGTGGTCGATATAGGCGCCACCATGACCACCCTGAGCGTGCTGCATCATGGCC
GGATCATTTATACCCGCGAGCAGCTGTTTGGCGGGCGCCAGCTGACTGACGAAATTCAGCGCCGCTACGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGACTACGTCAGCGAGGTGCTCGACCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGCTCCCTGCAGTTCTTCTTTGCGGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATCTGATCCAGCGGCGCATCGGCACTCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGTTCCAAGGTCAATGCCGGCGCCCTGGCCAGTGATGCGCCGGCGCTGATGATCGCGTGTGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
pilM	Acinetobacter baumannii D1279779	55.932	100	0.559
comM	Acinetobacter baylyi ADP1	54.237	100	0.542
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469