Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ITG10_RS04950 Genome accession   NZ_CP066149
Coordinates   1159600..1161285 (+) Length   561 a.a.
NCBI ID   WP_248386737.1    Uniprot ID   -
Organism   Vibrio sp. ED004     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1154600..1166285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG10_RS04930 (ITG10_04865) pdhR 1156071..1156832 (-) 762 WP_017629813.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ITG10_RS04935 (ITG10_04870) ampD 1157316..1157915 (-) 600 WP_017629812.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ITG10_RS04940 (ITG10_04875) nadC 1157999..1158889 (+) 891 WP_017629811.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ITG10_RS04945 (ITG10_04880) - 1159145..1159600 (+) 456 WP_017629810.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ITG10_RS04950 (ITG10_04885) pilB 1159600..1161285 (+) 1686 WP_248386737.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ITG10_RS04955 (ITG10_04890) pilC 1161301..1162530 (+) 1230 WP_017629808.1 type II secretion system F family protein Machinery gene
  ITG10_RS04960 (ITG10_04895) pilD 1162598..1163467 (+) 870 WP_017629807.1 A24 family peptidase Machinery gene
  ITG10_RS04965 (ITG10_04900) coaE 1163470..1164084 (+) 615 WP_017629806.1 dephospho-CoA kinase -
  ITG10_RS04970 (ITG10_04905) zapD 1164111..1164851 (+) 741 WP_017629805.1 cell division protein ZapD -
  ITG10_RS04975 (ITG10_04910) yacG 1164932..1165129 (+) 198 WP_017106879.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62275.89 Da        Isoelectric Point: 5.0476

>NTDB_id=517862 ITG10_RS04950 WP_248386737.1 1159600..1161285(+) (pilB) [Vibrio sp. ED004]
MLTNLPTVLRQADLLSLTQEQTVAEHIHTSGVSTPEALLVLDFFTSESLAHNIQAIFGLPSVQLANTDYEVLCDQLGLRE
LITKYHAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQGPKRKEITQDELAN
LVEVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKAEDDSQDIYLRHSIPNSQI
IYKANTQGCNECNQGYSGRVGIYEVMPFTDKLKNSLINKPNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
I

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=517862 ITG10_RS04950 WP_248386737.1 1159600..1161285(+) (pilB) [Vibrio sp. ED004]
ATGCTCACCAACCTCCCGACCGTTCTACGTCAGGCTGACTTACTTAGCCTGACTCAAGAACAAACCGTTGCGGAACACAT
ACATACTTCAGGTGTATCAACACCTGAAGCCTTACTCGTTCTTGATTTCTTCACTAGCGAGAGTCTCGCACATAACATTC
AGGCCATCTTCGGTCTGCCATCGGTGCAACTCGCCAATACTGACTACGAAGTCTTGTGCGACCAATTAGGGCTTCGCGAG
CTGATCACCAAGTATCACGCTATCCCGATTTCAGTGTCTAGTTCGACTCTCACATTAGCTTCAGCCGACCCGACCGATTT
ACAAGCGGAAGATGATTTCCGATTCGCGACTGGATTGCAGATAGAACTTGTTGTTGCTAACTACTCTGAATTAGAAGGCG
CAATACGAAAGTTGTATGGCCGCTCTATTTCAGGGCAAGGCCCTAAGCGTAAAGAGATCACCCAAGATGAACTCGCCAAC
CTGGTCGAAGTCTCCGACGATGAGATAACTTCAATTGAAGACCTCAGCCAAGACGACTCCCCCGTTAGCCGATTTATCAA
TCAGATACTGGTTGATGCGGTGCGCAAAGGTGCATCGGATATCCACTTTGAACCTTACGAAGAGCACTATCGTGTACGAC
TGCGTTGTGATGGTATTCTGGTTGAAATACAGCAACCCGCTTCCCATTTAAGTCGCCGTTTATCTGCTCGTCTGAAAATA
CTCGCCAAGCTTGATATCGCAGAACGTCGCTTGCCACAAGATGGACGCATCAAGCTGCGCCTGAATGATGAGCTCGCGAT
CGATATGCGTGTATCTACACTTCCGACCTTATGGGGAGAGAAGATAGTACTGCGACTGCTTGATAGCAGTGCGGCCAATC
TCGATATCGACAAGCTTGGCTACAGTGAAGATCAGAAAGCCCTATACCTTAATGCCTTGAAACGCCCGCAAGGAATGATC
CTAATGACAGGGCCAACCGGCAGTGGCAAAACCGTGTCTCTTTATACTGGGCTTCGAGTACTAAATACCACTGAACGCAA
TATTTCCACCGCTGAAGATCCGGTAGAGATTAACCTGTGTGGCATCAATCAGGTACAAGTGACACCAAAGATCGGCTTTG
GATTCGCGGAAGCACTTCGCTCGTTTTTACGACAAGACCCAGATGTAGTGATGGTCGGTGAGATCCGGGATTTAGAAACG
GCAGAGATCGCGATTAAAGCATCACAAACAGGGCACTTGGTGCTATCTACGTTGCACACCAACTCCGCAGCAGAAACGGT
GACTCGACTCGCTCACATGGGGATTGAACCCTTTAATCTTGCTTCGTCATTGAGCTTGATTATTGCTCAACGTCTTGCGA
GACGCTTATGTAACCATTGTAAAGCTGAAGACGATTCACAAGATATTTACCTGCGTCACTCTATCCCAAATAGCCAAATC
ATCTACAAAGCCAACACTCAAGGCTGTAATGAGTGCAATCAAGGATACTCCGGTCGCGTTGGTATCTACGAAGTAATGCC
ATTCACCGACAAACTTAAAAACAGCCTAATTAACAAACCTAATGCGTTAGCGATTGAAGATCTCGCCCGCCGAGAAGGCA
TGAGAACACTGCAAGAGTCTGGTTTAGACAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTGTAC
ATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.132

100

0.733
  pilB Vibrio campbellii strain DS40M4

70.213

100

0.706
  pilB Vibrio parahaemolyticus RIMD 2210633

70.107

100

0.702
  pilB Acinetobacter baumannii D1279779

49.811

94.118

0.469
  pilB Legionella pneumophila strain ERS1305867

49.237

93.405

0.46
  pilB Acinetobacter baylyi ADP1

48.679

94.474

0.46
  pilF Neisseria gonorrhoeae MS11

43.784

98.93

0.433
  pilF Thermus thermophilus HB27

39.155

92.87

0.364