Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I9192_RS20210 Genome accession   NZ_CP066119
Coordinates   4312371..4314086 (+) Length   571 a.a.
NCBI ID   WP_026040257.1    Uniprot ID   -
Organism   Acinetobacter bereziniae strain GD03185     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4307371..4319086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9192_RS20170 (I9192_20165) nusA 4308111..4309595 (-) 1485 WP_004826370.1 transcription termination factor NusA -
  I9192_RS20175 (I9192_20170) rimP 4309630..4310154 (-) 525 WP_004826368.1 ribosome maturation factor RimP -
  I9192_RS20200 (I9192_20195) secG 4310947..4311288 (-) 342 WP_005035388.1 preprotein translocase subunit SecG -
  I9192_RS20205 (I9192_20200) tpiA 4311298..4312092 (-) 795 WP_005035386.1 triose-phosphate isomerase -
  I9192_RS20210 (I9192_20205) pilB 4312371..4314086 (+) 1716 WP_026040257.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I9192_RS20215 (I9192_20210) pilC 4314114..4315343 (+) 1230 WP_005035382.1 type II secretion system F family protein Machinery gene
  I9192_RS20220 (I9192_20215) pilD 4315346..4316227 (+) 882 WP_005035380.1 A24 family peptidase Machinery gene
  I9192_RS20225 (I9192_20220) coaE 4316253..4316852 (+) 600 WP_005035377.1 dephospho-CoA kinase -
  I9192_RS20230 (I9192_20225) - 4316899..4317819 (-) 921 WP_005035375.1 DMT family transporter -
  I9192_RS20235 (I9192_20230) rlmB 4317860..4318612 (-) 753 WP_005035372.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I9192_RS20240 (I9192_20235) - 4318756..4319079 (-) 324 WP_004718357.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63400.85 Da        Isoelectric Point: 4.9070

>NTDB_id=517494 I9192_RS20210 WP_026040257.1 4312371..4314086(+) (pilB) [Acinetobacter bereziniae strain GD03185]
MSMQNGSPKFSGYIRRLVDEGFLSAQQMKDALTSAQKSKTHLVPYLIEKLAISPLLIAEMISSEFGEPIFDLDSFNPEML
PKDIVDAGLISKHSVIPLVQRGNILYVATSDPTNIEALDAIRFSCGLNIEPIIVEHHKLLKNLEKHFTVEESFNFGDEDF
DVDIIATDDAPPSDDDDEETSEEEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRTITNPPLQLANRLA
SRLKVMSQMDISEKRIPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALER
PQGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNLKAGLTFSVALKSFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNHCKAPIEIPKQSLLELG
FTEQDLNHPDLQIFQPMGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKLGFDNLRRSGLKKVMNGLT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=517494 I9192_RS20210 WP_026040257.1 4312371..4314086(+) (pilB) [Acinetobacter bereziniae strain GD03185]
ATGTCTATGCAAAATGGTTCACCTAAGTTTTCGGGTTATATCCGTCGCTTAGTCGATGAAGGCTTTTTATCTGCTCAGCA
AATGAAAGATGCCTTAACAAGCGCTCAAAAAAGTAAAACCCATTTGGTGCCTTACTTGATTGAGAAATTAGCGATCTCAC
CATTGCTGATCGCTGAAATGATCTCTTCCGAGTTCGGCGAGCCTATTTTTGATCTAGACAGTTTTAACCCTGAAATGTTG
CCCAAGGACATTGTTGACGCCGGTTTGATCAGCAAACATAGTGTGATTCCATTGGTGCAACGTGGTAATATCTTATATGT
TGCCACCAGCGACCCCACCAATATCGAGGCACTTGATGCGATTCGTTTTAGTTGTGGTCTAAACATTGAACCAATCATCG
TTGAACATCATAAATTATTAAAAAATTTAGAAAAGCATTTTACTGTAGAAGAAAGCTTTAATTTTGGCGATGAAGATTTT
GATGTCGATATTATTGCGACGGATGATGCTCCACCTTCAGATGACGATGATGAGGAAACTTCCGAAGAAGAAGCACCGAT
TGTTAAATATATCAATAAACTGTTGATTGATGCCATTCGAATGGGCGCCTCAGACTTACATTTTGAACCTTATGAAAAAA
GTTATCGTGTTCGCTATCGCGTAGATGGCGTACTGAGAACCATTACCAATCCCCCACTGCAATTGGCAAATCGTTTGGCA
TCCCGTTTAAAAGTCATGTCGCAAATGGATATTTCAGAGAAACGTATTCCTCAAGATGGGCGGATCAAACTTAAACTGTC
TAAAACCAAAGCTATTGATTTTCGTGTGAACTCACTTCCAACATTGTTTGGTGAAAAATTAGTTCTGCGTATTCTTGATC
CTTCCAGTGCCATGTTAGGGATTGATGCTTTGGGCTATGAAGAAGATCAAAAAGCTTTATTTATGGAAGCACTAGAAAGA
CCACAAGGCATGTTATTGATTACCGGTCCAACAGGTTCTGGTAAAACCGTATCTTTATATACTGGCTTAAATATCCTCAA
CACCGAAAGTTCCAATATTTCAACCGCAGAAGACCCTGTGGAGATTAATTTGGAGGGGATTAACCAAGTAAACGTCAATC
TTAAAGCAGGATTGACCTTTTCAGTCGCGCTAAAATCATTTCTTCGTCAAGATCCAGATATTATAATGGTCGGGGAGATT
CGAGACCTTGAAACGGCTGAAATTGCAATCAAAGCAGCCCAAACAGGTCATATGGTGATGTCCACACTGCATACCAATAG
TGCACCAGAAACCTTGACCCGATTGCGCAATATGGGTGTGCCATCCTTCAATATTGCAACATCGGTCAATCTTGTGATTG
CACAACGTCTTGCTCGACGTTTATGCAACCACTGTAAAGCACCTATTGAAATTCCGAAACAAAGTTTGTTAGAACTGGGC
TTTACTGAACAAGACTTAAACCATCCTGATTTACAGATTTTTCAGCCTATGGGCTGTTCTGAATGTAGAGAAGGTTATAA
AGGACGAGTCGGTATTTATGAGGTGATGAAAGTAACACCTGAAATTTCAAAAATTATCATGGAAGATGGCAACGCACTAC
AAATTGCAGCAGCATCTGAGAAATTGGGGTTTGATAATTTACGACGTTCAGGTTTAAAAAAGGTTATGAATGGTTTAACG
TCTTTACAAGAAATCAACCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.245

100

0.804
  pilB Acinetobacter baylyi ADP1

75.657

100

0.757
  pilB Legionella pneumophila strain ERS1305867

50.957

100

0.513
  pilF Neisseria gonorrhoeae MS11

49.296

99.475

0.49
  pilB Vibrio cholerae strain A1552

48.925

97.723

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

48.833

97.548

0.476
  pilB Vibrio campbellii strain DS40M4

47.756

97.548

0.466
  pilB/pilB1 Synechocystis sp. PCC 6803

34.641

100

0.371
  pilF Thermus thermophilus HB27

36.458

100

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.319

96.673

0.361