Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6H54_RS22975 Genome accession   NZ_CP066006
Coordinates   5019029..5020771 (+) Length   580 a.a.
NCBI ID   WP_034396919.1    Uniprot ID   -
Organism   Delftia acidovorans strain FDAARGOS_997     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5014029..5025771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H54_RS22945 (I6H54_22945) proB 5014547..5015695 (-) 1149 WP_012207281.1 glutamate 5-kinase -
  I6H54_RS22950 (I6H54_22950) cgtA 5015779..5016894 (-) 1116 WP_034396915.1 Obg family GTPase CgtA -
  I6H54_RS22955 (I6H54_22955) rpmA 5017044..5017301 (-) 258 WP_012207283.1 50S ribosomal protein L27 -
  I6H54_RS22960 (I6H54_22960) rplU 5017317..5017628 (-) 312 WP_012207284.1 50S ribosomal protein L21 -
  I6H54_RS22965 (I6H54_22965) - 5017859..5018788 (+) 930 WP_034396917.1 polyprenyl synthetase family protein -
  I6H54_RS22975 (I6H54_22975) pilB 5019029..5020771 (+) 1743 WP_034396919.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6H54_RS22980 (I6H54_22980) pilC 5020810..5022027 (+) 1218 WP_034396921.1 type II secretion system F family protein Machinery gene
  I6H54_RS22985 (I6H54_22985) pilD 5022027..5022914 (+) 888 WP_034396923.1 prepilin peptidase Machinery gene
  I6H54_RS22990 (I6H54_22990) coaE 5022929..5023573 (+) 645 WP_034396924.1 dephospho-CoA kinase -
  I6H54_RS22995 (I6H54_22995) zapD 5023663..5024418 (+) 756 WP_034396925.1 cell division protein ZapD -
  I6H54_RS23000 (I6H54_23000) - 5024418..5024684 (+) 267 WP_034398936.1 DNA gyrase inhibitor YacG -
  I6H54_RS23005 (I6H54_23005) - 5024695..5025213 (-) 519 WP_034396927.1 (deoxy)nucleoside triphosphate pyrophosphohydrolase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62773.90 Da        Isoelectric Point: 5.3458

>NTDB_id=516275 I6H54_RS22975 WP_034396919.1 5019029..5020771(+) (pilB) [Delftia acidovorans strain FDAARGOS_997]
MAVADSSLKNASAVASLPGIGRALISAGKVSQQAAEAAASKAGANRTSFIAELSNAGILSALEVAETVSTMFSAPLLDLD
AINAARLPHELLDAKIISSYRVLPLSKRGNRITVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLERLIAQQSKSASET
MESYSGGGDFEFGDLKPDEVAEEKDNTAATDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KARLIEAIGRPYGMILVTGPTGSGKTVSLYTCLNMLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALLEAGYDEADIDGSWVTYKPVGCQACNNGYKGRVGIYQVMPISEEIQRIILQDGSALEIAAQAKREGVRSLRGSG
LHKARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=516275 I6H54_RS22975 WP_034396919.1 5019029..5020771(+) (pilB) [Delftia acidovorans strain FDAARGOS_997]
ATGGCTGTTGCTGACTCCTCGCTCAAGAATGCTTCCGCTGTTGCATCGCTTCCCGGCATCGGGCGAGCCCTGATATCAGC
AGGCAAGGTATCGCAGCAGGCAGCGGAAGCCGCCGCCAGCAAGGCCGGCGCCAACCGCACCTCGTTCATTGCCGAGTTGA
GCAACGCGGGCATCCTCTCAGCCCTGGAAGTGGCCGAGACGGTCTCCACCATGTTCAGCGCGCCGCTGCTCGATCTTGAT
GCCATCAACGCCGCGCGCCTGCCGCATGAGTTGCTGGACGCCAAGATCATCAGCTCCTATCGCGTCCTCCCCTTGAGCAA
GCGAGGCAACCGCATCACGGTGGCCACGGCCGACCCGACACAGCAGGAAGCTGCAGAGCAGATCAAGTTCACCACCCAGC
TAGGCGTGGATTGGATCATCGTCGAGGCCGACAAGCTGGAACGCCTGATAGCTCAGCAGAGCAAAAGCGCCAGCGAAACC
ATGGAGAGCTACAGCGGGGGCGGCGATTTCGAGTTCGGCGACCTCAAGCCCGACGAGGTTGCCGAGGAGAAGGACAACAC
GGCGGCAACGGACGTCGAGGACGCGCCCATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAACATGCGCGCGT
CGGACCTGCATTTCGAGCCCTACGAACATCAGTACCGCGTGCGCTTCCGGATTGACGGCGAGTTGCGCGAGATTGCATCG
CCCCCCATTGCGATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCATGGACATCTCGGAAAAGCGGGTTCC
GCAAGACGGGCGCATGAAGCTCAAGGTGGGTCCGGACCGCGTCATCGATTTTCGCGTGAGCACCCTGCCCACGCTCTTCG
GCGAGAAGATCGTGATCCGTATCCTTGACCCGAGCTCGGCCAAGATGGGCATTGATGCCCTGGGCTACGAACCCGAGGAA
AAGGCACGTCTGATCGAAGCAATCGGGCGCCCCTACGGAATGATTCTGGTGACAGGCCCCACGGGCTCGGGCAAGACGGT
GTCGCTGTACACCTGCCTGAACATGCTGAACAAGGCCGGGGTGAACATCGCGACGGCAGAAGACCCCTCGGAAATCAACC
TGCCCGGTGTCAACCAGGTCAATGTGAACGAAAAGGCGGGCCTTACCTTTGCGACGGCGCTCAAGGCCTTTCTGCGCCAG
GATCCCGACATCATCATGGTCGGTGAAATCCGTGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACCGGTCA
CTTGGTGCTGTCCACGCTGCACACCAACGACGCACCGACCACGTTGACGCGCATGCGCAACATGGGCATTGCCCCCTTCA
ACATCGCATCGAGCGTGATCCTGATCACCGCCCAGCGGCTGGCCAGGCGCCTGTGCCCCCAGTGCAAGGAGCCTGCGGAT
ATCCCGCGGGAGGCGCTGCTGGAGGCGGGCTATGACGAGGCGGACATCGATGGCAGCTGGGTCACCTACAAGCCCGTGGG
CTGCCAGGCATGCAACAACGGCTACAAGGGACGTGTCGGCATCTACCAGGTGATGCCGATCAGCGAAGAAATCCAGCGCA
TCATCCTGCAGGACGGCAGCGCGCTGGAGATCGCGGCACAGGCCAAGCGGGAAGGCGTTCGGTCTCTGCGCGGATCCGGA
CTGCACAAGGCCAGACTAGGACTGACCTCGCTCGAAGAAGTGCTGGCAGTTACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.712

98.103

0.547
  pilB Acinetobacter baumannii D1279779

56.028

97.241

0.545
  pilF Neisseria gonorrhoeae MS11

52.12

97.586

0.509
  pilB Legionella pneumophila strain ERS1305867

50.439

98.103

0.495
  pilB Vibrio cholerae strain A1552

46.279

95

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

45.372

95

0.431
  pilB Vibrio campbellii strain DS40M4

43.54

97.414

0.424
  pilF Thermus thermophilus HB27

38.028

97.931

0.372