Detailed information NTG0514732

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: I8T81_RS01660
• Genome accession: NZ_CP065820
• Coordinates: 348309..349367 (+)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain S21
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 343309..354367

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I8T81_RS01645 (I8T81_01645)	-	344416..344748 (+)	333	WP_077163639.1	hypothetical protein	-
I8T81_RS01650 (I8T81_01650)	-	344751..345575 (+)	825	WP_198071983.1	putative RNA methyltransferase	-
I8T81_RS01655 (I8T81_01655)	ponA	345592..348147 (-)	2556	WP_198071984.1	penicillin-binding protein PBP1a	-
I8T81_RS01660 (I8T81_01660)	pilM	348309..349367 (+)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
I8T81_RS01665 (I8T81_01665)	pilN	349367..350011 (+)	645	WP_004705190.1	PilN domain-containing protein	Machinery gene
I8T81_RS01670 (I8T81_01670)	pilO	350008..350748 (+)	741	WP_198071985.1	type 4a pilus biogenesis protein PilO	Machinery gene
I8T81_RS01675 (I8T81_01675)	pilP	350759..351286 (+)	528	WP_198071986.1	pilus assembly protein PilP	Machinery gene
I8T81_RS01680 (I8T81_01680)	pilQ	351348..353474 (+)	2127	WP_151760756.1	type IV pilus secretin PilQ family protein	Machinery gene
I8T81_RS01685 (I8T81_01685)	aroK	353486..354055 (+)	570	WP_004705195.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=514732 I8T81_RS01660 WP_004705187.1 348309..349367(+) (pilM) [Acinetobacter seifertii strain S21]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=514732 I8T81_RS01660 WP_004705187.1 348309..349367(+) (pilM) [Acinetobacter seifertii strain S21]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTGAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACCAAAAATGCTGCAATT
GCTGTTCCTACATCGACAGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGTCTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGAGTTTTAGATATTGGTCATACCATGACAACCTTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGTGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGCTCTTTACCAGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTG
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426