Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   I8T81_RS01665 Genome accession   NZ_CP065820
Coordinates   349367..350011 (+) Length   214 a.a.
NCBI ID   WP_004705190.1    Uniprot ID   N8R2F5
Organism   Acinetobacter seifertii strain S21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 344367..355011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8T81_RS01645 (I8T81_01645) - 344416..344748 (+) 333 WP_077163639.1 hypothetical protein -
  I8T81_RS01650 (I8T81_01650) - 344751..345575 (+) 825 WP_198071983.1 putative RNA methyltransferase -
  I8T81_RS01655 (I8T81_01655) ponA 345592..348147 (-) 2556 WP_198071984.1 penicillin-binding protein PBP1a -
  I8T81_RS01660 (I8T81_01660) pilM 348309..349367 (+) 1059 WP_004705187.1 pilus assembly protein PilM Machinery gene
  I8T81_RS01665 (I8T81_01665) pilN 349367..350011 (+) 645 WP_004705190.1 PilN domain-containing protein Machinery gene
  I8T81_RS01670 (I8T81_01670) pilO 350008..350748 (+) 741 WP_198071985.1 type 4a pilus biogenesis protein PilO Machinery gene
  I8T81_RS01675 (I8T81_01675) pilP 350759..351286 (+) 528 WP_198071986.1 pilus assembly protein PilP Machinery gene
  I8T81_RS01680 (I8T81_01680) pilQ 351348..353474 (+) 2127 WP_151760756.1 type IV pilus secretin PilQ family protein Machinery gene
  I8T81_RS01685 (I8T81_01685) aroK 353486..354055 (+) 570 WP_004705195.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 214 a.a.        Molecular weight: 24168.55 Da        Isoelectric Point: 5.3957

>NTDB_id=514733 I8T81_RS01665 WP_004705190.1 349367..350011(+) (pilN) [Acinetobacter seifertii strain S21]
MATINLLPWREELREQRKKQFITLCFGVVVLGITTVFGGWFYFDHKLDDQEQANQLITSTNQNLDQQLKALNGLQEQRDA
IIERMKLIQDLQSQRPVVVRLVDELVRVTPPAMYLTKFSRTGDKFTIEGKAESPNTVAELLRNLEASPWYRNAFMNSFLA
TEDKKDKAASSLLPRVEDHYGSFVVTVDLGEMGMTTADNNADKPAVAKDTGAAK

Nucleotide


Download         Length: 645 bp        

>NTDB_id=514733 I8T81_RS01665 WP_004705190.1 349367..350011(+) (pilN) [Acinetobacter seifertii strain S21]
ATGGCAACAATTAATTTACTACCTTGGCGTGAAGAGCTGAGGGAGCAACGAAAAAAACAATTTATAACGTTATGCTTTGG
GGTGGTTGTGTTAGGAATTACAACCGTATTTGGGGGGTGGTTCTATTTTGATCATAAATTAGATGATCAAGAACAAGCAA
ATCAACTTATTACAAGTACTAATCAAAACCTTGATCAGCAATTAAAAGCCTTAAATGGTTTACAAGAACAACGTGATGCC
ATCATTGAGCGTATGAAATTGATTCAAGATTTACAGAGTCAGCGTCCAGTAGTGGTAAGACTGGTTGATGAGTTAGTCAG
AGTCACGCCGCCTGCAATGTATTTAACGAAATTTAGCCGAACTGGAGATAAATTTACGATTGAAGGTAAAGCTGAAAGTC
CAAATACTGTAGCTGAACTACTGCGTAATCTTGAAGCATCTCCTTGGTATCGAAATGCTTTTATGAACTCTTTCTTGGCT
ACTGAAGATAAAAAAGATAAGGCAGCAAGTTCTCTTCTTCCTAGGGTAGAAGATCATTACGGTAGTTTTGTTGTGACTGT
CGATTTAGGGGAAATGGGCATGACCACAGCTGATAATAATGCTGATAAACCAGCCGTAGCTAAAGATACGGGGGCAGCCA
AATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N8R2F5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

92.056

100

0.921
  comN Acinetobacter baylyi ADP1

70.616

98.598

0.696